NUTRACEUTICALS IMPROVE EXPERIMENTAL COLITIS: PLACE OF CATECHINS IN THE 2,4,6-TRINITROBENZENE SULFONIC ACID MODEL RATS

Risultato della ricerca: Other

Abstract

Background: Nutraceuticals provide added health benefits for inflammatory bowel disese (IBD) and Epigallocatechin-3-galate (EGCG), a green tea catechin, has been shown to possess such anti-inflammatory and anti-oxidant effects. Aim: To evaluate the molecular modulation of Reactive Oxygen Species (ROS), Tumor Necrosis Factor-alpha (TNF_alpha), nuclear factor Kappa Beta (NF-Kbeta), Interleukin 6 (IL-6) by EGCG on experimental colitis. Matherial and Methods: Thirthy five male sprague-dawley rats were randomly divided into 4 groups: Normal control group (n=5), EGCG group (n=9), TNBS group (n=9), and TNBS + EGCG group (n=12). For both TNBS and EGCG treated groups, 1 mg/Kg EGCG was administered daily by intraperitoneal injection, starting one week before the induction. at days 3, 10, and 17, rats were sacrificed and the descending colon was collected. The mRNA expressions of IL-6, TNF-alpha and NF-KB were measured by the polymerase chain reaction. In addition, the oxidative stress wasstudied by determination of ROS expression using dihydroethinidine (DHE) staining. results: The results showed that EGCG caused a significant decrease in the mRNA expression of pro-inflammatory cytokines IL-6, TNF-alpha and NF-KB compared to respective non-treated groups. In addition, ROS expression was reduced. Conclusions: These data provide evidence that EGCG is beneficial in TNBS-induced colitis; it exerts an antioxidant activity via decreasing ROS, and an anti-inflammatory effect via ameliorating histological findings and reducing IL-6, TNF-alpha and NF-KB.
Lingua originaleEnglish
Pagine95-95
Numero di pagine1
Stato di pubblicazionePublished - 2017

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Sulfonic Acids
Catechin
Colitis
Dietary Supplements
Interleukin-6
Reactive Oxygen Species
Tumor Necrosis Factor-alpha
Anti-Inflammatory Agents
Antioxidants
Descending Colon
Messenger RNA
gallocatechol
sym-trinitrobenzene
Insurance Benefits
Tea
Intraperitoneal Injections
Sprague Dawley Rats
Oxidative Stress
Staining and Labeling
Cytokines

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@conference{12f23a93804548a9863611850c9315d6,
title = "NUTRACEUTICALS IMPROVE EXPERIMENTAL COLITIS: PLACE OF CATECHINS IN THE 2,4,6-TRINITROBENZENE SULFONIC ACID MODEL RATS",
abstract = "Background: Nutraceuticals provide added health benefits for inflammatory bowel disese (IBD) and Epigallocatechin-3-galate (EGCG), a green tea catechin, has been shown to possess such anti-inflammatory and anti-oxidant effects. Aim: To evaluate the molecular modulation of Reactive Oxygen Species (ROS), Tumor Necrosis Factor-alpha (TNF_alpha), nuclear factor Kappa Beta (NF-Kbeta), Interleukin 6 (IL-6) by EGCG on experimental colitis. Matherial and Methods: Thirthy five male sprague-dawley rats were randomly divided into 4 groups: Normal control group (n=5), EGCG group (n=9), TNBS group (n=9), and TNBS + EGCG group (n=12). For both TNBS and EGCG treated groups, 1 mg/Kg EGCG was administered daily by intraperitoneal injection, starting one week before the induction. at days 3, 10, and 17, rats were sacrificed and the descending colon was collected. The mRNA expressions of IL-6, TNF-alpha and NF-KB were measured by the polymerase chain reaction. In addition, the oxidative stress wasstudied by determination of ROS expression using dihydroethinidine (DHE) staining. results: The results showed that EGCG caused a significant decrease in the mRNA expression of pro-inflammatory cytokines IL-6, TNF-alpha and NF-KB compared to respective non-treated groups. In addition, ROS expression was reduced. Conclusions: These data provide evidence that EGCG is beneficial in TNBS-induced colitis; it exerts an antioxidant activity via decreasing ROS, and an anti-inflammatory effect via ameliorating histological findings and reducing IL-6, TNF-alpha and NF-KB.",
keywords = "2, 4, 6-trinitrobenzene sulfonic acid, experimental colitis, inflammatory bowel diseases, model rats",
author = "Giovanni Tomasello and Angelo Leone and Francesco Cappello",
year = "2017",
language = "English",
pages = "95--95",

}

TY - CONF

T1 - NUTRACEUTICALS IMPROVE EXPERIMENTAL COLITIS: PLACE OF CATECHINS IN THE 2,4,6-TRINITROBENZENE SULFONIC ACID MODEL RATS

AU - Tomasello, Giovanni

AU - Leone, Angelo

AU - Cappello, Francesco

PY - 2017

Y1 - 2017

N2 - Background: Nutraceuticals provide added health benefits for inflammatory bowel disese (IBD) and Epigallocatechin-3-galate (EGCG), a green tea catechin, has been shown to possess such anti-inflammatory and anti-oxidant effects. Aim: To evaluate the molecular modulation of Reactive Oxygen Species (ROS), Tumor Necrosis Factor-alpha (TNF_alpha), nuclear factor Kappa Beta (NF-Kbeta), Interleukin 6 (IL-6) by EGCG on experimental colitis. Matherial and Methods: Thirthy five male sprague-dawley rats were randomly divided into 4 groups: Normal control group (n=5), EGCG group (n=9), TNBS group (n=9), and TNBS + EGCG group (n=12). For both TNBS and EGCG treated groups, 1 mg/Kg EGCG was administered daily by intraperitoneal injection, starting one week before the induction. at days 3, 10, and 17, rats were sacrificed and the descending colon was collected. The mRNA expressions of IL-6, TNF-alpha and NF-KB were measured by the polymerase chain reaction. In addition, the oxidative stress wasstudied by determination of ROS expression using dihydroethinidine (DHE) staining. results: The results showed that EGCG caused a significant decrease in the mRNA expression of pro-inflammatory cytokines IL-6, TNF-alpha and NF-KB compared to respective non-treated groups. In addition, ROS expression was reduced. Conclusions: These data provide evidence that EGCG is beneficial in TNBS-induced colitis; it exerts an antioxidant activity via decreasing ROS, and an anti-inflammatory effect via ameliorating histological findings and reducing IL-6, TNF-alpha and NF-KB.

AB - Background: Nutraceuticals provide added health benefits for inflammatory bowel disese (IBD) and Epigallocatechin-3-galate (EGCG), a green tea catechin, has been shown to possess such anti-inflammatory and anti-oxidant effects. Aim: To evaluate the molecular modulation of Reactive Oxygen Species (ROS), Tumor Necrosis Factor-alpha (TNF_alpha), nuclear factor Kappa Beta (NF-Kbeta), Interleukin 6 (IL-6) by EGCG on experimental colitis. Matherial and Methods: Thirthy five male sprague-dawley rats were randomly divided into 4 groups: Normal control group (n=5), EGCG group (n=9), TNBS group (n=9), and TNBS + EGCG group (n=12). For both TNBS and EGCG treated groups, 1 mg/Kg EGCG was administered daily by intraperitoneal injection, starting one week before the induction. at days 3, 10, and 17, rats were sacrificed and the descending colon was collected. The mRNA expressions of IL-6, TNF-alpha and NF-KB were measured by the polymerase chain reaction. In addition, the oxidative stress wasstudied by determination of ROS expression using dihydroethinidine (DHE) staining. results: The results showed that EGCG caused a significant decrease in the mRNA expression of pro-inflammatory cytokines IL-6, TNF-alpha and NF-KB compared to respective non-treated groups. In addition, ROS expression was reduced. Conclusions: These data provide evidence that EGCG is beneficial in TNBS-induced colitis; it exerts an antioxidant activity via decreasing ROS, and an anti-inflammatory effect via ameliorating histological findings and reducing IL-6, TNF-alpha and NF-KB.

KW - 2

KW - 4

KW - 6-trinitrobenzene sulfonic acid

KW - experimental colitis

KW - inflammatory bowel diseases

KW - model rats

UR - http://hdl.handle.net/10447/225057

M3 - Other

SP - 95

EP - 95

ER -