Abstract

Recently, NiII and ZnII metal complexes of the ligand Salpyrim have been synthesized and characterized. Theiraffinity for wild-type h-Telo G-quadruplex DNA and for calf thymus DNA was investigated by UV absorptionspectroscopy, circular dichroism and viscometry. The data collectively suggest that both complexes bindeffectively to G-quadruplexes by direct end-stacking, stabilizing the oligonucleotide secondary structure. Thetwo complexes are also typical B-DNA intercalators. Remarkably, their binding constants, Kb, with the G4sstructures are about 10 fold higher than those with B-DNA, highlighting the selectivity. Experiments toevaluate the biological activity of the two complexes against MCF7 and HeLa cancer cell lines are currentlyongoing.
Lingua originaleEnglish
Numero di pagine1
Stato di pubblicazionePublished - 2014

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B-Form DNA
Intercalating Agents
Schiff Bases
Coordination Complexes
Viscosity measurement
Bioactivity
Oligonucleotides
DNA
Cells
Ligands
Experiments
calf thymus DNA

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@conference{e3b460e838a04a96a7514daa21386eb7,
title = "NiII, and ZnII Schiff Base Complexes: Telomeric G-quadruplex Stabilizers",
abstract = "Recently, NiII and ZnII metal complexes of the ligand Salpyrim have been synthesized and characterized. Theiraffinity for wild-type h-Telo G-quadruplex DNA and for calf thymus DNA was investigated by UV absorptionspectroscopy, circular dichroism and viscometry. The data collectively suggest that both complexes bindeffectively to G-quadruplexes by direct end-stacking, stabilizing the oligonucleotide secondary structure. Thetwo complexes are also typical B-DNA intercalators. Remarkably, their binding constants, Kb, with the G4sstructures are about 10 fold higher than those with B-DNA, highlighting the selectivity. Experiments toevaluate the biological activity of the two complexes against MCF7 and HeLa cancer cell lines are currentlyongoing.",
author = "Carla Gentile and Almerico, {Anna Maria} and Angelo Spinello and Antonino Lauria and Alessio Terenzi and Annamaria Martorana and Giampaolo Barone and Riccardo Bonsignore",
year = "2014",
language = "English",

}

TY - CONF

T1 - NiII, and ZnII Schiff Base Complexes: Telomeric G-quadruplex Stabilizers

AU - Gentile, Carla

AU - Almerico, Anna Maria

AU - Spinello, Angelo

AU - Lauria, Antonino

AU - Terenzi, Alessio

AU - Martorana, Annamaria

AU - Barone, Giampaolo

AU - Bonsignore, Riccardo

PY - 2014

Y1 - 2014

N2 - Recently, NiII and ZnII metal complexes of the ligand Salpyrim have been synthesized and characterized. Theiraffinity for wild-type h-Telo G-quadruplex DNA and for calf thymus DNA was investigated by UV absorptionspectroscopy, circular dichroism and viscometry. The data collectively suggest that both complexes bindeffectively to G-quadruplexes by direct end-stacking, stabilizing the oligonucleotide secondary structure. Thetwo complexes are also typical B-DNA intercalators. Remarkably, their binding constants, Kb, with the G4sstructures are about 10 fold higher than those with B-DNA, highlighting the selectivity. Experiments toevaluate the biological activity of the two complexes against MCF7 and HeLa cancer cell lines are currentlyongoing.

AB - Recently, NiII and ZnII metal complexes of the ligand Salpyrim have been synthesized and characterized. Theiraffinity for wild-type h-Telo G-quadruplex DNA and for calf thymus DNA was investigated by UV absorptionspectroscopy, circular dichroism and viscometry. The data collectively suggest that both complexes bindeffectively to G-quadruplexes by direct end-stacking, stabilizing the oligonucleotide secondary structure. Thetwo complexes are also typical B-DNA intercalators. Remarkably, their binding constants, Kb, with the G4sstructures are about 10 fold higher than those with B-DNA, highlighting the selectivity. Experiments toevaluate the biological activity of the two complexes against MCF7 and HeLa cancer cell lines are currentlyongoing.

UR - http://hdl.handle.net/10447/98673

M3 - Other

ER -