NEW PEGYLATED POLYASPARTAMIDE-BASED POLYPLEXES AS GENE DELIVERY VECTORS

Risultato della ricerca: Article

14 Citazioni (Scopus)

Abstract

Aims: To synthesize novel polyhydroxyethylaspartamide (PHEA) copolymers containing spermine (Spm) and polyethylene glycol (PEG) moieties in high yields, with the expectation that this material would show stealth properties and the ability to complex DNA by electrostatic interactions. Material & methods: PHEA-PEG-Spm copolymer was prepared with a two-step reaction. Chemical, physicochemical and biological characterizations of PHEA-PEG-Spm copolymers and their obtained polyplexes with pDNA were performed. Results: The introduction of spermine in PHEA structure allows to obtain a copolymer bearing in the side chain polyamine moieties capable to interact with DNA. On the other hand, the introduction of PEG in polymeric structure increased the DNA condensing ability of PHEA-PEG-Spm copolymer in comparison with the derivatives without PEG (PHEA-Spm), and improved its characteristics of biocompatibility. Conclusions: PHEA-PEG-Spm copolymer shows excellent ability to complex and condense plasmidic DNA giving interpolyelectrolyte complexes to act as gene delivery systems. Moreover, PEGylation confers to the obtained interpolyelectrolyte complexes stealth properties.
Lingua originaleEnglish
pagine (da-a)243-258
Numero di pagine16
RivistaNanomedicine
Volume5
Stato di pubblicazionePublished - 2010

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Spermine
Polyethylene glycols
Genes
Copolymers
DNA
gene
ability
Bearings (structural)
Gene Transfer Techniques
interaction
Polyamines
Coulomb interactions
Static Electricity
Biocompatibility
Derivatives
material

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Materials Science(all)
  • Biomedical Engineering
  • Medicine (miscellaneous)

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NEW PEGYLATED POLYASPARTAMIDE-BASED POLYPLEXES AS GENE DELIVERY VECTORS. /.

In: Nanomedicine, Vol. 5, 2010, pag. 243-258.

Risultato della ricerca: Article

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title = "NEW PEGYLATED POLYASPARTAMIDE-BASED POLYPLEXES AS GENE DELIVERY VECTORS",
abstract = "Aims: To synthesize novel polyhydroxyethylaspartamide (PHEA) copolymers containing spermine (Spm) and polyethylene glycol (PEG) moieties in high yields, with the expectation that this material would show stealth properties and the ability to complex DNA by electrostatic interactions. Material & methods: PHEA-PEG-Spm copolymer was prepared with a two-step reaction. Chemical, physicochemical and biological characterizations of PHEA-PEG-Spm copolymers and their obtained polyplexes with pDNA were performed. Results: The introduction of spermine in PHEA structure allows to obtain a copolymer bearing in the side chain polyamine moieties capable to interact with DNA. On the other hand, the introduction of PEG in polymeric structure increased the DNA condensing ability of PHEA-PEG-Spm copolymer in comparison with the derivatives without PEG (PHEA-Spm), and improved its characteristics of biocompatibility. Conclusions: PHEA-PEG-Spm copolymer shows excellent ability to complex and condense plasmidic DNA giving interpolyelectrolyte complexes to act as gene delivery systems. Moreover, PEGylation confers to the obtained interpolyelectrolyte complexes stealth properties.",
keywords = "GENE DELIVERY, VECTORS, POLYPLEXES, NANOMEDICINE.",
author = "Gaetano Giammona and Gennara Cavallaro and Mariano Licciardi and Sergio Scire' and {Di Stefano}, Mauro",
year = "2010",
language = "English",
volume = "5",
pages = "243--258",
journal = "Nanomedicine",
issn = "1743-5889",
publisher = "Future Medicine Ltd.",

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TY - JOUR

T1 - NEW PEGYLATED POLYASPARTAMIDE-BASED POLYPLEXES AS GENE DELIVERY VECTORS

AU - Giammona, Gaetano

AU - Cavallaro, Gennara

AU - Licciardi, Mariano

AU - Scire', Sergio

AU - Di Stefano, Mauro

PY - 2010

Y1 - 2010

N2 - Aims: To synthesize novel polyhydroxyethylaspartamide (PHEA) copolymers containing spermine (Spm) and polyethylene glycol (PEG) moieties in high yields, with the expectation that this material would show stealth properties and the ability to complex DNA by electrostatic interactions. Material & methods: PHEA-PEG-Spm copolymer was prepared with a two-step reaction. Chemical, physicochemical and biological characterizations of PHEA-PEG-Spm copolymers and their obtained polyplexes with pDNA were performed. Results: The introduction of spermine in PHEA structure allows to obtain a copolymer bearing in the side chain polyamine moieties capable to interact with DNA. On the other hand, the introduction of PEG in polymeric structure increased the DNA condensing ability of PHEA-PEG-Spm copolymer in comparison with the derivatives without PEG (PHEA-Spm), and improved its characteristics of biocompatibility. Conclusions: PHEA-PEG-Spm copolymer shows excellent ability to complex and condense plasmidic DNA giving interpolyelectrolyte complexes to act as gene delivery systems. Moreover, PEGylation confers to the obtained interpolyelectrolyte complexes stealth properties.

AB - Aims: To synthesize novel polyhydroxyethylaspartamide (PHEA) copolymers containing spermine (Spm) and polyethylene glycol (PEG) moieties in high yields, with the expectation that this material would show stealth properties and the ability to complex DNA by electrostatic interactions. Material & methods: PHEA-PEG-Spm copolymer was prepared with a two-step reaction. Chemical, physicochemical and biological characterizations of PHEA-PEG-Spm copolymers and their obtained polyplexes with pDNA were performed. Results: The introduction of spermine in PHEA structure allows to obtain a copolymer bearing in the side chain polyamine moieties capable to interact with DNA. On the other hand, the introduction of PEG in polymeric structure increased the DNA condensing ability of PHEA-PEG-Spm copolymer in comparison with the derivatives without PEG (PHEA-Spm), and improved its characteristics of biocompatibility. Conclusions: PHEA-PEG-Spm copolymer shows excellent ability to complex and condense plasmidic DNA giving interpolyelectrolyte complexes to act as gene delivery systems. Moreover, PEGylation confers to the obtained interpolyelectrolyte complexes stealth properties.

KW - GENE DELIVERY, VECTORS, POLYPLEXES, NANOMEDICINE.

UR - http://hdl.handle.net/10447/53508

M3 - Article

VL - 5

SP - 243

EP - 258

JO - Nanomedicine

JF - Nanomedicine

SN - 1743-5889

ER -