Neutrophil extracellular traps mediate transfer of cytoplasmic neutrophil antigens to myeloid dendritic cells toward ANCA induction and associated autoimmunity.

Carla Guarnotta, Claudio Tripodo, Cristiana Guiducci, Caterina Vitali, Patrizia Casalini, Patrizia Casalini, Sabina Sangaletti, Barbara Cappetti, Silvia Piconese, Claudia Chiodoni, Mariella Parenza, Patrizia Casalini, Mario P. Colombo

Risultato della ricerca: Article

223 Citazioni (Scopus)

Abstract

Antineutrophil cytoplasmic antibodies (ANCAs) target proteins normally retained within neutrophils, indicating that cell death is involved in the autoimmunity process. Still, ANCA pathogenesis remains obscure. ANCAs activate neutrophils inducing their respiratory burst and a peculiar form of cell death, named NETosis, characterized by formation of neutrophil extracellular traps (NETs), decondensed chromatin threads decorated with cytoplasmic proteins endorsed with antimicrobial activity. NETs have been consistently detected in ANCA-associated small-vessel vasculitis, and this association prompted us to test whether the peculiar structure of NET favors neutrophil proteins uploading into myeloid dendritic cells and the induction of ANCAs and associated autoimmunity. Here we show that myeloid DCs uploaded with and activated by NET components induce ANCA and autoimmunity when injected into naive mice. DC uploading and autoimmunity induction are prevented by NET treatment with DNAse, indicating that NET structural integrity is needed to maintain the antigenicity of cytoplasmic proteins. We found NET intermingling with myeloid dendritic cells also positive for neutrophil myeloperoxidase in myeloperoxidase-ANCA-associated microscopic poliangiitis providing a potential correlative picture in human pathology. These data provide the first demonstration that NET structures are highly immunogenic such to trigger adaptive immune response relevant for autoimmunity.
Lingua originaleEnglish
pagine (da-a)3007-3018
Numero di pagine12
RivistaBlood
Volume120
Stato di pubblicazionePublished - 2012

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Guarnotta, C., Tripodo, C., Guiducci, C., Vitali, C., Casalini, P., Casalini, P., Sangaletti, S., Cappetti, B., Piconese, S., Chiodoni, C., Parenza, M., Casalini, P., & Colombo, M. P. (2012). Neutrophil extracellular traps mediate transfer of cytoplasmic neutrophil antigens to myeloid dendritic cells toward ANCA induction and associated autoimmunity. Blood, 120, 3007-3018.