Abstract
| Lingua originale | English |
|---|---|
| pagine (da-a) | 183-190 |
| Numero di pagine | 8 |
| Rivista | European Journal of Pharmacology |
| Volume | 460 |
| Stato di pubblicazione | Published - 2003 |
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All Science Journal Classification (ASJC) codes
- Pharmacology
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Neurotransmitters involved in the fast inhibitory junction potentials in mouse distal colon. / Serio, Rosa Maria; Mule', Flavia; Zizzo, Maria Grazia; Tamburello, Maria Pia.
In: European Journal of Pharmacology, Vol. 460, 2003, pag. 183-190.Risultato della ricerca: Article
}
TY - JOUR
T1 - Neurotransmitters involved in the fast inhibitory junction potentials in mouse distal colon
AU - Serio, Rosa Maria
AU - Mule', Flavia
AU - Zizzo, Maria Grazia
AU - Tamburello, Maria Pia
PY - 2003
Y1 - 2003
N2 - We investigated, in murine colon circular muscle, the role of adenosine 5′-triphosphate (ATP) and pituitary adenylate cyclase activating peptide (PACAP) as inhibitory neurotransmitters of the fast component of nerve-evoked inhibitory junction potential (fast IJP). Fast IJP was antagonised by apamin or suramin, abolished by desensitisation with the P2Y receptor agonist, adenosine 5′-O-2-thiodiphosphate (ADPβS), unaffected by desensitisation with P2X receptor agonist, α,β-methylene ATP (α,β-meATP), and reduced by PACAP-(6-38), a PACAP receptor antagonist. ATP induced membrane hyperpolarization resistant to tetrodotoxin, Nω-nitro-L-arginine methyl ester (L-NAME) or PACAP-(6-38), but antagonised by apamin, suramin, P2X and P2Y receptor desensitisation. PACAP-(1-27) caused membrane hyperpolarization antagonised by PACAP-(6-38), apamin and P2Y receptor desensitisation, reduced by tetrodotoxin, but not affected by L-NAME and by P2X receptor desensitisation. Therefore, in murine colon circular muscle, an ATP-like endogenous P2Y purinoceptor ligand is the final nonadrenergic, noncholinergic (NANC) inhibitory mediator involved in the generation of fast IJP. A neuromodulator role of PACAP in the inhibitory pathway is supposed. © 2003 Elsevier Science B.V. All rights reserved.
AB - We investigated, in murine colon circular muscle, the role of adenosine 5′-triphosphate (ATP) and pituitary adenylate cyclase activating peptide (PACAP) as inhibitory neurotransmitters of the fast component of nerve-evoked inhibitory junction potential (fast IJP). Fast IJP was antagonised by apamin or suramin, abolished by desensitisation with the P2Y receptor agonist, adenosine 5′-O-2-thiodiphosphate (ADPβS), unaffected by desensitisation with P2X receptor agonist, α,β-methylene ATP (α,β-meATP), and reduced by PACAP-(6-38), a PACAP receptor antagonist. ATP induced membrane hyperpolarization resistant to tetrodotoxin, Nω-nitro-L-arginine methyl ester (L-NAME) or PACAP-(6-38), but antagonised by apamin, suramin, P2X and P2Y receptor desensitisation. PACAP-(1-27) caused membrane hyperpolarization antagonised by PACAP-(6-38), apamin and P2Y receptor desensitisation, reduced by tetrodotoxin, but not affected by L-NAME and by P2X receptor desensitisation. Therefore, in murine colon circular muscle, an ATP-like endogenous P2Y purinoceptor ligand is the final nonadrenergic, noncholinergic (NANC) inhibitory mediator involved in the generation of fast IJP. A neuromodulator role of PACAP in the inhibitory pathway is supposed. © 2003 Elsevier Science B.V. All rights reserved.
KW - ATP
KW - Apamin
KW - Cellular and Molecular Neuroscience
KW - Colon
KW - Fast inhibitory junction potential
KW - Murine
KW - PACAP (pituitary adenylate cyclase activating peptide)
KW - Pharmacology
KW - Purinoceptor
UR - http://hdl.handle.net/10447/199037
M3 - Article
VL - 460
SP - 183
EP - 190
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
ER -