Neurotransmitters involved in the fast inhibitory junction potentials in mouse distal colon

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Abstract

We investigated, in murine colon circular muscle, the role of adenosine 5′-triphosphate (ATP) and pituitary adenylate cyclase activating peptide (PACAP) as inhibitory neurotransmitters of the fast component of nerve-evoked inhibitory junction potential (fast IJP). Fast IJP was antagonised by apamin or suramin, abolished by desensitisation with the P2Y receptor agonist, adenosine 5′-O-2-thiodiphosphate (ADPβS), unaffected by desensitisation with P2X receptor agonist, α,β-methylene ATP (α,β-meATP), and reduced by PACAP-(6-38), a PACAP receptor antagonist. ATP induced membrane hyperpolarization resistant to tetrodotoxin, Nω-nitro-L-arginine methyl ester (L-NAME) or PACAP-(6-38), but antagonised by apamin, suramin, P2X and P2Y receptor desensitisation. PACAP-(1-27) caused membrane hyperpolarization antagonised by PACAP-(6-38), apamin and P2Y receptor desensitisation, reduced by tetrodotoxin, but not affected by L-NAME and by P2X receptor desensitisation. Therefore, in murine colon circular muscle, an ATP-like endogenous P2Y purinoceptor ligand is the final nonadrenergic, noncholinergic (NANC) inhibitory mediator involved in the generation of fast IJP. A neuromodulator role of PACAP in the inhibitory pathway is supposed. © 2003 Elsevier Science B.V. All rights reserved.
Lingua originaleEnglish
pagine (da-a)183-190
Numero di pagine8
RivistaEuropean Journal of Pharmacology
Volume460
Stato di pubblicazionePublished - 2003

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Neurotransmitter Agents
Colon
Adenosine Triphosphate
Adenylyl Cyclases
Apamin
Suramin
NG-Nitroarginine Methyl Ester
Tetrodotoxin
Peptides
Purinergic P2Y Receptors
Pituitary Adenylate Cyclase-Activating Polypeptide Receptors
Muscles
Membranes
Ligands
pituitary adenylate-cyclase-activating-peptide (6-38)

All Science Journal Classification (ASJC) codes

  • Pharmacology

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title = "Neurotransmitters involved in the fast inhibitory junction potentials in mouse distal colon",
abstract = "We investigated, in murine colon circular muscle, the role of adenosine 5′-triphosphate (ATP) and pituitary adenylate cyclase activating peptide (PACAP) as inhibitory neurotransmitters of the fast component of nerve-evoked inhibitory junction potential (fast IJP). Fast IJP was antagonised by apamin or suramin, abolished by desensitisation with the P2Y receptor agonist, adenosine 5′-O-2-thiodiphosphate (ADPβS), unaffected by desensitisation with P2X receptor agonist, α,β-methylene ATP (α,β-meATP), and reduced by PACAP-(6-38), a PACAP receptor antagonist. ATP induced membrane hyperpolarization resistant to tetrodotoxin, Nω-nitro-L-arginine methyl ester (L-NAME) or PACAP-(6-38), but antagonised by apamin, suramin, P2X and P2Y receptor desensitisation. PACAP-(1-27) caused membrane hyperpolarization antagonised by PACAP-(6-38), apamin and P2Y receptor desensitisation, reduced by tetrodotoxin, but not affected by L-NAME and by P2X receptor desensitisation. Therefore, in murine colon circular muscle, an ATP-like endogenous P2Y purinoceptor ligand is the final nonadrenergic, noncholinergic (NANC) inhibitory mediator involved in the generation of fast IJP. A neuromodulator role of PACAP in the inhibitory pathway is supposed. {\circledC} 2003 Elsevier Science B.V. All rights reserved.",
keywords = "ATP, Apamin, Cellular and Molecular Neuroscience, Colon, Fast inhibitory junction potential, Murine, PACAP (pituitary adenylate cyclase activating peptide), Pharmacology, Purinoceptor",
author = "Serio, {Rosa Maria} and Flavia Mule' and Zizzo, {Maria Grazia} and Tamburello, {Maria Pia}",
year = "2003",
language = "English",
volume = "460",
pages = "183--190",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",

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TY - JOUR

T1 - Neurotransmitters involved in the fast inhibitory junction potentials in mouse distal colon

AU - Serio, Rosa Maria

AU - Mule', Flavia

AU - Zizzo, Maria Grazia

AU - Tamburello, Maria Pia

PY - 2003

Y1 - 2003

N2 - We investigated, in murine colon circular muscle, the role of adenosine 5′-triphosphate (ATP) and pituitary adenylate cyclase activating peptide (PACAP) as inhibitory neurotransmitters of the fast component of nerve-evoked inhibitory junction potential (fast IJP). Fast IJP was antagonised by apamin or suramin, abolished by desensitisation with the P2Y receptor agonist, adenosine 5′-O-2-thiodiphosphate (ADPβS), unaffected by desensitisation with P2X receptor agonist, α,β-methylene ATP (α,β-meATP), and reduced by PACAP-(6-38), a PACAP receptor antagonist. ATP induced membrane hyperpolarization resistant to tetrodotoxin, Nω-nitro-L-arginine methyl ester (L-NAME) or PACAP-(6-38), but antagonised by apamin, suramin, P2X and P2Y receptor desensitisation. PACAP-(1-27) caused membrane hyperpolarization antagonised by PACAP-(6-38), apamin and P2Y receptor desensitisation, reduced by tetrodotoxin, but not affected by L-NAME and by P2X receptor desensitisation. Therefore, in murine colon circular muscle, an ATP-like endogenous P2Y purinoceptor ligand is the final nonadrenergic, noncholinergic (NANC) inhibitory mediator involved in the generation of fast IJP. A neuromodulator role of PACAP in the inhibitory pathway is supposed. © 2003 Elsevier Science B.V. All rights reserved.

AB - We investigated, in murine colon circular muscle, the role of adenosine 5′-triphosphate (ATP) and pituitary adenylate cyclase activating peptide (PACAP) as inhibitory neurotransmitters of the fast component of nerve-evoked inhibitory junction potential (fast IJP). Fast IJP was antagonised by apamin or suramin, abolished by desensitisation with the P2Y receptor agonist, adenosine 5′-O-2-thiodiphosphate (ADPβS), unaffected by desensitisation with P2X receptor agonist, α,β-methylene ATP (α,β-meATP), and reduced by PACAP-(6-38), a PACAP receptor antagonist. ATP induced membrane hyperpolarization resistant to tetrodotoxin, Nω-nitro-L-arginine methyl ester (L-NAME) or PACAP-(6-38), but antagonised by apamin, suramin, P2X and P2Y receptor desensitisation. PACAP-(1-27) caused membrane hyperpolarization antagonised by PACAP-(6-38), apamin and P2Y receptor desensitisation, reduced by tetrodotoxin, but not affected by L-NAME and by P2X receptor desensitisation. Therefore, in murine colon circular muscle, an ATP-like endogenous P2Y purinoceptor ligand is the final nonadrenergic, noncholinergic (NANC) inhibitory mediator involved in the generation of fast IJP. A neuromodulator role of PACAP in the inhibitory pathway is supposed. © 2003 Elsevier Science B.V. All rights reserved.

KW - ATP

KW - Apamin

KW - Cellular and Molecular Neuroscience

KW - Colon

KW - Fast inhibitory junction potential

KW - Murine

KW - PACAP (pituitary adenylate cyclase activating peptide)

KW - Pharmacology

KW - Purinoceptor

UR - http://hdl.handle.net/10447/199037

M3 - Article

VL - 460

SP - 183

EP - 190

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

ER -