63 Citazioni (Scopus)

Abstract

We previously found that neurons are able to affect the ability of brain capillary endothelial cells to form in vitro a monolayer with properties resembling the blood-brain barrier. We then looked, by immunofluorescence and western analysis, for factors, produced by neurons, with the potential to influence growth and differentiation of endothelial cells. In the present paper, we report that neurons produce both vascular endothelial growth factor and fibroblast growth factor 2, two well-known angiogenic factors. More interestingly, we gained evidence that both factors are released by neurons, at least in part, by shedding of extracellular vesicles, that contain beta1 integrin, a membrane protein already known to be part of extracellular vesicles released by tumour cells. Shedding of extracellular vesicles by neurons was also confirmed by scanner electron microscopy.
Lingua originaleEnglish
pagine (da-a)1-12
Numero di pagine13
RivistaJournal of Cellular and Molecular Medicine
Volume2007
Stato di pubblicazionePublished - 2007

Fingerprint

Fibroblast Growth Factor 2
Vascular Endothelial Growth Factor A
Neurons
Endothelial cells
CD29 Antigens
Endothelial Cells
Angiogenesis Inducing Agents
Blood-Brain Barrier
Electron microscopy
Statistical Factor Analysis
Fluorescent Antibody Technique
Tumors
Monolayers
Brain
Electron Microscopy
Membrane Proteins
Cells
Growth
Extracellular Vesicles
Neoplasms

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Cell Biology

Cita questo

@article{0fcf590a7859474d911ae997c6e89ec0,
title = "NEURONS PRODUCE FGF-2 AND VEGF SECRETE THEM AT LEST IN PART BY SHEDDING EXTRACELLULAR VESCICLES",
abstract = "We previously found that neurons are able to affect the ability of brain capillary endothelial cells to form in vitro a monolayer with properties resembling the blood-brain barrier. We then looked, by immunofluorescence and western analysis, for factors, produced by neurons, with the potential to influence growth and differentiation of endothelial cells. In the present paper, we report that neurons produce both vascular endothelial growth factor and fibroblast growth factor 2, two well-known angiogenic factors. More interestingly, we gained evidence that both factors are released by neurons, at least in part, by shedding of extracellular vesicles, that contain beta1 integrin, a membrane protein already known to be part of extracellular vesicles released by tumour cells. Shedding of extracellular vesicles by neurons was also confirmed by scanner electron microscopy.",
keywords = "fibroblastic growth factor-2, neurons, vascular endothelial growth factor, vesicles",
author = "Patrizia Proia and Giovanni Savettieri and {Di Liegro}, Italia and Gabriella Schiera and {Di Liegro}, Italia and Marco Mineo",
year = "2007",
language = "English",
volume = "2007",
pages = "1--12",
journal = "Journal of Cellular and Molecular Medicine",
issn = "1582-1838",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - NEURONS PRODUCE FGF-2 AND VEGF SECRETE THEM AT LEST IN PART BY SHEDDING EXTRACELLULAR VESCICLES

AU - Proia, Patrizia

AU - Savettieri, Giovanni

AU - Di Liegro, Italia

AU - Schiera, Gabriella

AU - Di Liegro, Italia

AU - Mineo, Marco

PY - 2007

Y1 - 2007

N2 - We previously found that neurons are able to affect the ability of brain capillary endothelial cells to form in vitro a monolayer with properties resembling the blood-brain barrier. We then looked, by immunofluorescence and western analysis, for factors, produced by neurons, with the potential to influence growth and differentiation of endothelial cells. In the present paper, we report that neurons produce both vascular endothelial growth factor and fibroblast growth factor 2, two well-known angiogenic factors. More interestingly, we gained evidence that both factors are released by neurons, at least in part, by shedding of extracellular vesicles, that contain beta1 integrin, a membrane protein already known to be part of extracellular vesicles released by tumour cells. Shedding of extracellular vesicles by neurons was also confirmed by scanner electron microscopy.

AB - We previously found that neurons are able to affect the ability of brain capillary endothelial cells to form in vitro a monolayer with properties resembling the blood-brain barrier. We then looked, by immunofluorescence and western analysis, for factors, produced by neurons, with the potential to influence growth and differentiation of endothelial cells. In the present paper, we report that neurons produce both vascular endothelial growth factor and fibroblast growth factor 2, two well-known angiogenic factors. More interestingly, we gained evidence that both factors are released by neurons, at least in part, by shedding of extracellular vesicles, that contain beta1 integrin, a membrane protein already known to be part of extracellular vesicles released by tumour cells. Shedding of extracellular vesicles by neurons was also confirmed by scanner electron microscopy.

KW - fibroblastic growth factor-2

KW - neurons

KW - vascular endothelial growth factor

KW - vesicles

UR - http://hdl.handle.net/10447/36887

M3 - Article

VL - 2007

SP - 1

EP - 12

JO - Journal of Cellular and Molecular Medicine

JF - Journal of Cellular and Molecular Medicine

SN - 1582-1838

ER -