Nandrolone decanoate interferes with testosterone biosynthesis altering blood-testis barrier components

Francesco Cappello, Francesca Di Gaudio, Valentina Di Felice, Alessandro Pitruzzella, Antonella Marino Gammazza, Rosario Barone, Francesca Rappa, Claudia Sangiorgi, Daniela D'Amico, Francesca Di Gaudio, Luigi Cipolloni, Venerando Rapisarda, Cristoforo Pomara, Valentina Di Felice, Antonella Marino Gammazza, Stefania Schiavone, Gabriele Sani, Alessandro Pitruzzella, Nicola Locorotondo, Emanuela TurillazziMonica Salerno, Fulvio Barone, Amos Tambo

Risultato della ricerca: Article

9 Citazioni (Scopus)

Abstract

The aim of this study was to investigate whether nandrolone decanoate (ND) use affects testosterone production and testicular morphology in a model of trained and sedentary mice. A group of mice underwent endurance training while another set led a sedentary lifestyle and were freely mobile within cages. All experimental groups were treated with either ND or peanut oil at different doses for 6 weeks. Testosterone serum levels were measured via liquid chromatography-mass spectrometry. Western blot analysis and quantitative real-time PCR were utilized to determine gene and protein expression levels of the primary enzymes implicated in testosterone biosynthesis and gene expression levels of the blood-testis barrier (BTB) components. Immunohistochemistry and immunofluorescence were conducted for testicular morphological evaluation. The study demonstrated that moderate to high doses of ND induced a diminished serum testosterone level and altered the expression level of the key steroidogenic enzymes involved in testosterone biosynthesis. At the morphological level, ND induced degradation of the BTB by targeting the tight junction protein-1 (TJP1). ND stimulation deregulated metalloproteinase-9, metalloproteinase-2 (MMP-2) and the tissue inhibitor of MMP-2. Moreover, ND administration resulted in a mislocalization of mucin-1. In conclusion, ND abuse induces a decline in testosterone production that is unable to regulate the internalization and redistribution of TJP1 and may induce the deregulation of other BTB constituents via the inhibition of MMP-2. ND may well be considered as both a potential inducer of male infertility and a potential risk factor to a low endogenous bioavailable testosterone.
Lingua originaleEnglish
pagine (da-a)-
Numero di pagine12
RivistaJournal of Cellular and Molecular Medicine
Stato di pubblicazionePublished - 2017

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Blood-Testis Barrier
Testosterone
Metalloproteases
Zonula Occludens-1 Protein
Sedentary Lifestyle
Gene Expression
Tissue Inhibitor of Metalloproteinase-2
Mucin-1
nandrolone decanoate
Male Infertility
Enzymes
Serum
Liquid Chromatography
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Mass Spectrometry
Western Blotting
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Cell Biology

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Nandrolone decanoate interferes with testosterone biosynthesis altering blood-testis barrier components. / Cappello, Francesco; Di Gaudio, Francesca; Di Felice, Valentina; Pitruzzella, Alessandro; Marino Gammazza, Antonella; Barone, Rosario; Rappa, Francesca; Sangiorgi, Claudia; D'Amico, Daniela; Di Gaudio, Francesca; Cipolloni, Luigi; Rapisarda, Venerando; Pomara, Cristoforo; Di Felice, Valentina; Marino Gammazza, Antonella; Schiavone, Stefania; Sani, Gabriele; Pitruzzella, Alessandro; Locorotondo, Nicola; Turillazzi, Emanuela; Salerno, Monica; Barone, Fulvio; Tambo, Amos.

In: Journal of Cellular and Molecular Medicine, 2017, pag. -.

Risultato della ricerca: Article

Cappello, F, Di Gaudio, F, Di Felice, V, Pitruzzella, A, Marino Gammazza, A, Barone, R, Rappa, F, Sangiorgi, C, D'Amico, D, Di Gaudio, F, Cipolloni, L, Rapisarda, V, Pomara, C, Di Felice, V, Marino Gammazza, A, Schiavone, S, Sani, G, Pitruzzella, A, Locorotondo, N, Turillazzi, E, Salerno, M, Barone, F & Tambo, A 2017, 'Nandrolone decanoate interferes with testosterone biosynthesis altering blood-testis barrier components', Journal of Cellular and Molecular Medicine, pagg. -.
Cappello, Francesco ; Di Gaudio, Francesca ; Di Felice, Valentina ; Pitruzzella, Alessandro ; Marino Gammazza, Antonella ; Barone, Rosario ; Rappa, Francesca ; Sangiorgi, Claudia ; D'Amico, Daniela ; Di Gaudio, Francesca ; Cipolloni, Luigi ; Rapisarda, Venerando ; Pomara, Cristoforo ; Di Felice, Valentina ; Marino Gammazza, Antonella ; Schiavone, Stefania ; Sani, Gabriele ; Pitruzzella, Alessandro ; Locorotondo, Nicola ; Turillazzi, Emanuela ; Salerno, Monica ; Barone, Fulvio ; Tambo, Amos. / Nandrolone decanoate interferes with testosterone biosynthesis altering blood-testis barrier components. In: Journal of Cellular and Molecular Medicine. 2017 ; pagg. -.
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abstract = "The aim of this study was to investigate whether nandrolone decanoate (ND) use affects testosterone production and testicular morphology in a model of trained and sedentary mice. A group of mice underwent endurance training while another set led a sedentary lifestyle and were freely mobile within cages. All experimental groups were treated with either ND or peanut oil at different doses for 6 weeks. Testosterone serum levels were measured via liquid chromatography-mass spectrometry. Western blot analysis and quantitative real-time PCR were utilized to determine gene and protein expression levels of the primary enzymes implicated in testosterone biosynthesis and gene expression levels of the blood-testis barrier (BTB) components. Immunohistochemistry and immunofluorescence were conducted for testicular morphological evaluation. The study demonstrated that moderate to high doses of ND induced a diminished serum testosterone level and altered the expression level of the key steroidogenic enzymes involved in testosterone biosynthesis. At the morphological level, ND induced degradation of the BTB by targeting the tight junction protein-1 (TJP1). ND stimulation deregulated metalloproteinase-9, metalloproteinase-2 (MMP-2) and the tissue inhibitor of MMP-2. Moreover, ND administration resulted in a mislocalization of mucin-1. In conclusion, ND abuse induces a decline in testosterone production that is unable to regulate the internalization and redistribution of TJP1 and may induce the deregulation of other BTB constituents via the inhibition of MMP-2. ND may well be considered as both a potential inducer of male infertility and a potential risk factor to a low endogenous bioavailable testosterone.",
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author = "Francesco Cappello and {Di Gaudio}, Francesca and {Di Felice}, Valentina and Alessandro Pitruzzella and {Marino Gammazza}, Antonella and Rosario Barone and Francesca Rappa and Claudia Sangiorgi and Daniela D'Amico and {Di Gaudio}, Francesca and Luigi Cipolloni and Venerando Rapisarda and Cristoforo Pomara and {Di Felice}, Valentina and {Marino Gammazza}, Antonella and Stefania Schiavone and Gabriele Sani and Alessandro Pitruzzella and Nicola Locorotondo and Emanuela Turillazzi and Monica Salerno and Fulvio Barone and Amos Tambo",
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TY - JOUR

T1 - Nandrolone decanoate interferes with testosterone biosynthesis altering blood-testis barrier components

AU - Cappello, Francesco

AU - Di Gaudio, Francesca

AU - Di Felice, Valentina

AU - Pitruzzella, Alessandro

AU - Marino Gammazza, Antonella

AU - Barone, Rosario

AU - Rappa, Francesca

AU - Sangiorgi, Claudia

AU - D'Amico, Daniela

AU - Di Gaudio, Francesca

AU - Cipolloni, Luigi

AU - Rapisarda, Venerando

AU - Pomara, Cristoforo

AU - Di Felice, Valentina

AU - Marino Gammazza, Antonella

AU - Schiavone, Stefania

AU - Sani, Gabriele

AU - Pitruzzella, Alessandro

AU - Locorotondo, Nicola

AU - Turillazzi, Emanuela

AU - Salerno, Monica

AU - Barone, Fulvio

AU - Tambo, Amos

PY - 2017

Y1 - 2017

N2 - The aim of this study was to investigate whether nandrolone decanoate (ND) use affects testosterone production and testicular morphology in a model of trained and sedentary mice. A group of mice underwent endurance training while another set led a sedentary lifestyle and were freely mobile within cages. All experimental groups were treated with either ND or peanut oil at different doses for 6 weeks. Testosterone serum levels were measured via liquid chromatography-mass spectrometry. Western blot analysis and quantitative real-time PCR were utilized to determine gene and protein expression levels of the primary enzymes implicated in testosterone biosynthesis and gene expression levels of the blood-testis barrier (BTB) components. Immunohistochemistry and immunofluorescence were conducted for testicular morphological evaluation. The study demonstrated that moderate to high doses of ND induced a diminished serum testosterone level and altered the expression level of the key steroidogenic enzymes involved in testosterone biosynthesis. At the morphological level, ND induced degradation of the BTB by targeting the tight junction protein-1 (TJP1). ND stimulation deregulated metalloproteinase-9, metalloproteinase-2 (MMP-2) and the tissue inhibitor of MMP-2. Moreover, ND administration resulted in a mislocalization of mucin-1. In conclusion, ND abuse induces a decline in testosterone production that is unable to regulate the internalization and redistribution of TJP1 and may induce the deregulation of other BTB constituents via the inhibition of MMP-2. ND may well be considered as both a potential inducer of male infertility and a potential risk factor to a low endogenous bioavailable testosterone.

AB - The aim of this study was to investigate whether nandrolone decanoate (ND) use affects testosterone production and testicular morphology in a model of trained and sedentary mice. A group of mice underwent endurance training while another set led a sedentary lifestyle and were freely mobile within cages. All experimental groups were treated with either ND or peanut oil at different doses for 6 weeks. Testosterone serum levels were measured via liquid chromatography-mass spectrometry. Western blot analysis and quantitative real-time PCR were utilized to determine gene and protein expression levels of the primary enzymes implicated in testosterone biosynthesis and gene expression levels of the blood-testis barrier (BTB) components. Immunohistochemistry and immunofluorescence were conducted for testicular morphological evaluation. The study demonstrated that moderate to high doses of ND induced a diminished serum testosterone level and altered the expression level of the key steroidogenic enzymes involved in testosterone biosynthesis. At the morphological level, ND induced degradation of the BTB by targeting the tight junction protein-1 (TJP1). ND stimulation deregulated metalloproteinase-9, metalloproteinase-2 (MMP-2) and the tissue inhibitor of MMP-2. Moreover, ND administration resulted in a mislocalization of mucin-1. In conclusion, ND abuse induces a decline in testosterone production that is unable to regulate the internalization and redistribution of TJP1 and may induce the deregulation of other BTB constituents via the inhibition of MMP-2. ND may well be considered as both a potential inducer of male infertility and a potential risk factor to a low endogenous bioavailable testosterone.

KW - Blood-testis barrier; MMP-2; MMP-9; MUC1; Nandrolone decanoate; Testosterone; TJP1; Molecular Medicine; Cell Biology

UR - http://hdl.handle.net/10447/225171

UR - http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1582-4934

M3 - Article

SP - -

JO - Journal of Cellular and Molecular Medicine

JF - Journal of Cellular and Molecular Medicine

SN - 1582-1838

ER -