Multiple in vitro and in vivo regulatory effects of budesonide in CD4+ T lymphocyte subpopulations of allergic asthmatics.

Stefania La Grutta, Serena Di Vincenzo, Carina Gabriela Uasuf, Maria Ferraro, Jean Bousquet, Serena Di Vincenzo, Mark Gjomarkaj, Giuseppe Liotta, Caterina Di Sano, Giuseppe Albeggiani, Giuseppe Albeggiani, Stefania La Grutta, Elisabetta Pace

    Risultato della ricerca: Article

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    Abstract

    AbstractBACKGROUND:Increased activation and increased survival of T lymphocytes characterise bronchial asthma.OBJECTIVES:In this study the effect of budesonide on T cell survival, on inducible co-stimulator T cells (ICOS), on Foxp3 and on IL-10 molecules in T lymphocyte sub-populations was assessed.METHODS:Cell survival (by annexin V binding) and ICOS in total lymphocytes, in CD4+/CD25+ and in CD4+/CD25- and Foxp3 and IL-10 in CD4+/CD25+ and in CD4+/CD25-cells was evaluated, by cytofluorimetric analysis, in mild intermittent asthmatics (n = 19) and in controls (n = 15). Allergen induced T lymphocyte proliferation and the in vivo effects of budesonide in mild persistent asthmatics (n = 6) were also explored.RESULTS:Foxp3 was reduced in CD4+/CD25- and in CD4+/CD25+ cells and ICOS was reduced in CD4+/CD25+ cells but it was increased in CD4+CD25-in asthmatics when compared to controls. In asthmatics, in vitro, budesonide was able to: 1) increase annexin V binding and to reduce ICOS in total lymphocytes; 2) increase annexin V binding and Foxp3 and to reduce ICOS in CD4+/CD25- cells; 3) reduce annexin V binding and to increase IL-10 and ICOS in CD4+/CD25+ cells; 4) reduce cell allergen induced proliferation. In vivo, budesonide increased ICOS in CD4+/CD25+ while it increased Foxp3 and IL-10 in CD4+/CD25+ and in CD4+/CD25- cells.CONCLUSIONS:Budesonide modulates T cell survival, ICOS, Foxp3 and IL-10 molecules differently in T lymphocyte sub-populations. The findings provided shed light on new mechanisms by which corticosteroids, drugs widely used for the clinical management of bronchial asthma, control T lymphocyte activation.
    Lingua originaleEnglish
    Numero di pagine12
    RivistaPLoS One
    Volume7
    Stato di pubblicazionePublished - 2012

    All Science Journal Classification (ASJC) codes

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)
    • General

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    La Grutta, S., Di Vincenzo, S., Uasuf, C. G., Ferraro, M., Bousquet, J., Di Vincenzo, S., Gjomarkaj, M., Liotta, G., Di Sano, C., Albeggiani, G., Albeggiani, G., La Grutta, S., & Pace, E. (2012). Multiple in vitro and in vivo regulatory effects of budesonide in CD4+ T lymphocyte subpopulations of allergic asthmatics. PLoS One, 7.