Multilocus microsatellite analysis of European and African Candida glabrata isolates

Antonio Cascio, Orazio Romeo, Scordino, Chillemi, Nnadi, Sampaio, Cilo, Rharmitt, Carla Lo Lo Passo, Van Diepeningen, Criseo, Pemán, Demetrio Delfino, Tietz, Cascio

Risultato della ricerca: Article

4 Citazioni (Scopus)

Abstract

This study aimed to elucidate the genetic relatedness and epidemiology of 127 clinical and environmental Candida glabrata isolates from Europe and Africa using multilocus microsatellite analysis. Each isolate was first identified using phenotypic and molecular methods and subsequently, six unlinked microsatellite loci were analyzed using automated fluorescent genotyping. Genetic relationships were estimated using the minimum-spanning tree (MStree) method. Microsatellite analyses revealed the existence of 47 different genotypes. The fungal population showed an irregular distribution owing to the over-representation of genetically different infectious haplotypes. The most common genotype was MG-9, which was frequently found in both European and African isolates. In conclusion, the data reported here emphasize the role of specific C. glabrata genotypes in human infections for at least some decades and highlight the widespread distribution of some isolates, which seem to be more able to cause disease than others.
Lingua originaleEnglish
pagine (da-a)885-892
Numero di pagine8
RivistaEUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
Volume35
Stato di pubblicazionePublished - 2016

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Candida glabrata
Microsatellite Repeats
Genotype
Molecular Epidemiology
Haplotypes
Infection
Population

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cita questo

Multilocus microsatellite analysis of European and African Candida glabrata isolates. / Cascio, Antonio; Romeo, Orazio; Scordino; Chillemi; Nnadi; Sampaio; Cilo; Rharmitt; Lo Passo, Carla Lo; Van Diepeningen; Criseo; Pemán; Delfino, Demetrio; Tietz; Cascio.

In: EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, Vol. 35, 2016, pag. 885-892.

Risultato della ricerca: Article

Cascio, A, Romeo, O, Scordino, Chillemi, Nnadi, Sampaio, Cilo, Rharmitt, Lo Passo, CL, Van Diepeningen, Criseo, Pemán, Delfino, D, Tietz & Cascio 2016, 'Multilocus microsatellite analysis of European and African Candida glabrata isolates', EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, vol. 35, pagg. 885-892.
Cascio, Antonio ; Romeo, Orazio ; Scordino ; Chillemi ; Nnadi ; Sampaio ; Cilo ; Rharmitt ; Lo Passo, Carla Lo ; Van Diepeningen ; Criseo ; Pemán ; Delfino, Demetrio ; Tietz ; Cascio. / Multilocus microsatellite analysis of European and African Candida glabrata isolates. In: EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES. 2016 ; Vol. 35. pagg. 885-892.
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title = "Multilocus microsatellite analysis of European and African Candida glabrata isolates",
abstract = "This study aimed to elucidate the genetic relatedness and epidemiology of 127 clinical and environmental Candida glabrata isolates from Europe and Africa using multilocus microsatellite analysis. Each isolate was first identified using phenotypic and molecular methods and subsequently, six unlinked microsatellite loci were analyzed using automated fluorescent genotyping. Genetic relationships were estimated using the minimum-spanning tree (MStree) method. Microsatellite analyses revealed the existence of 47 different genotypes. The fungal population showed an irregular distribution owing to the over-representation of genetically different infectious haplotypes. The most common genotype was MG-9, which was frequently found in both European and African isolates. In conclusion, the data reported here emphasize the role of specific C. glabrata genotypes in human infections for at least some decades and highlight the widespread distribution of some isolates, which seem to be more able to cause disease than others.",
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author = "Antonio Cascio and Orazio Romeo and Scordino and Chillemi and Nnadi and Sampaio and Cilo and Rharmitt and {Lo Passo}, {Carla Lo} and {Van Diepeningen} and Criseo and Pem{\'a}n and Demetrio Delfino and Tietz and Cascio",
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T1 - Multilocus microsatellite analysis of European and African Candida glabrata isolates

AU - Cascio, Antonio

AU - Romeo, Orazio

AU - Scordino, null

AU - Chillemi, null

AU - Nnadi, null

AU - Sampaio, null

AU - Cilo, null

AU - Rharmitt, null

AU - Lo Passo, Carla Lo

AU - Van Diepeningen, null

AU - Criseo, null

AU - Pemán, null

AU - Delfino, Demetrio

AU - Tietz, null

AU - Cascio, null

PY - 2016

Y1 - 2016

N2 - This study aimed to elucidate the genetic relatedness and epidemiology of 127 clinical and environmental Candida glabrata isolates from Europe and Africa using multilocus microsatellite analysis. Each isolate was first identified using phenotypic and molecular methods and subsequently, six unlinked microsatellite loci were analyzed using automated fluorescent genotyping. Genetic relationships were estimated using the minimum-spanning tree (MStree) method. Microsatellite analyses revealed the existence of 47 different genotypes. The fungal population showed an irregular distribution owing to the over-representation of genetically different infectious haplotypes. The most common genotype was MG-9, which was frequently found in both European and African isolates. In conclusion, the data reported here emphasize the role of specific C. glabrata genotypes in human infections for at least some decades and highlight the widespread distribution of some isolates, which seem to be more able to cause disease than others.

AB - This study aimed to elucidate the genetic relatedness and epidemiology of 127 clinical and environmental Candida glabrata isolates from Europe and Africa using multilocus microsatellite analysis. Each isolate was first identified using phenotypic and molecular methods and subsequently, six unlinked microsatellite loci were analyzed using automated fluorescent genotyping. Genetic relationships were estimated using the minimum-spanning tree (MStree) method. Microsatellite analyses revealed the existence of 47 different genotypes. The fungal population showed an irregular distribution owing to the over-representation of genetically different infectious haplotypes. The most common genotype was MG-9, which was frequently found in both European and African isolates. In conclusion, the data reported here emphasize the role of specific C. glabrata genotypes in human infections for at least some decades and highlight the widespread distribution of some isolates, which seem to be more able to cause disease than others.

KW - Africa; Alleles; Candida glabrata; Candidiasis; Environmental Microbiology; Europe; Genetic Loci; Genetic Variation; Genotype; Haplotypes; Humans; DNA

KW - Fungal; Microsatellite Repeats; Multilocus Sequence Typing; Microbiology (medical); Infectious Diseases

UR - http://hdl.handle.net/10447/246582

M3 - Article

VL - 35

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JO - EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES

JF - EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES

SN - 0934-9723

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