Multifibrillar bundles of a self-assembling hyaluronic acid derivative obtained through a microfluidic technique for aortic smooth muscle cell orientation and differentiation

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Abstract

A hyaluronic acid derivative that is able to physically crosslink in a saline aqueous environment was employed for the production of fibers with a mean diameter of 50 μm using a microfluidic technique. The microfibers were collected in a tailored rotating collector and assembled to form multifibrillar bundles. The orientation of the microfibers on the collected bundles was evaluated by microCT analysis. The bundles were biofunctionalized by physical addition of fibronectin or chemical tethering of a cyRGDC peptide to achieve control of Aortic Smooth Muscle Cell (AoSMC) attachment, elongation and alignment. The mechanical performances of these bundles were evaluated by elongation tests, related to the kind of biological functionalization and compared to non-functionalized samples. The alignment and differentiation of AoSMCs on single fibers and on the bundles were evaluated by microscopy and histochemical analyses.
Lingua originaleEnglish
pagine (da-a)2518-2526
Numero di pagine9
RivistaBiomaterials Science
Volume6
Stato di pubblicazionePublished - 2018

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Hyaluronic acid
Hyaluronic Acid
Microfluidics
Muscle
Elongation
Cells
Derivatives
Fibers
Fibronectins
Peptides
Microscopic examination

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Materials Science(all)

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title = "Multifibrillar bundles of a self-assembling hyaluronic acid derivative obtained through a microfluidic technique for aortic smooth muscle cell orientation and differentiation",
abstract = "A hyaluronic acid derivative that is able to physically crosslink in a saline aqueous environment was employed for the production of fibers with a mean diameter of 50 μm using a microfluidic technique. The microfibers were collected in a tailored rotating collector and assembled to form multifibrillar bundles. The orientation of the microfibers on the collected bundles was evaluated by microCT analysis. The bundles were biofunctionalized by physical addition of fibronectin or chemical tethering of a cyRGDC peptide to achieve control of Aortic Smooth Muscle Cell (AoSMC) attachment, elongation and alignment. The mechanical performances of these bundles were evaluated by elongation tests, related to the kind of biological functionalization and compared to non-functionalized samples. The alignment and differentiation of AoSMCs on single fibers and on the bundles were evaluated by microscopy and histochemical analyses.",
author = "Emanuela Bologna and Calogero Fiorica and Gaetano Giammona and Giovanna Pitarresi and Palumbo, {Fabio Salvatore} and Massimiliano Zingales and Emanuela Bologna and Massimiliano Zingales and Gaetano Giammona",
year = "2018",
language = "English",
volume = "6",
pages = "2518--2526",
journal = "Biomaterials Science",
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publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Multifibrillar bundles of a self-assembling hyaluronic acid derivative obtained through a microfluidic technique for aortic smooth muscle cell orientation and differentiation

AU - Bologna, Emanuela

AU - Fiorica, Calogero

AU - Giammona, Gaetano

AU - Pitarresi, Giovanna

AU - Palumbo, Fabio Salvatore

AU - Zingales, Massimiliano

AU - Bologna, Emanuela

AU - Zingales, Massimiliano

AU - Giammona, Gaetano

PY - 2018

Y1 - 2018

N2 - A hyaluronic acid derivative that is able to physically crosslink in a saline aqueous environment was employed for the production of fibers with a mean diameter of 50 μm using a microfluidic technique. The microfibers were collected in a tailored rotating collector and assembled to form multifibrillar bundles. The orientation of the microfibers on the collected bundles was evaluated by microCT analysis. The bundles were biofunctionalized by physical addition of fibronectin or chemical tethering of a cyRGDC peptide to achieve control of Aortic Smooth Muscle Cell (AoSMC) attachment, elongation and alignment. The mechanical performances of these bundles were evaluated by elongation tests, related to the kind of biological functionalization and compared to non-functionalized samples. The alignment and differentiation of AoSMCs on single fibers and on the bundles were evaluated by microscopy and histochemical analyses.

AB - A hyaluronic acid derivative that is able to physically crosslink in a saline aqueous environment was employed for the production of fibers with a mean diameter of 50 μm using a microfluidic technique. The microfibers were collected in a tailored rotating collector and assembled to form multifibrillar bundles. The orientation of the microfibers on the collected bundles was evaluated by microCT analysis. The bundles were biofunctionalized by physical addition of fibronectin or chemical tethering of a cyRGDC peptide to achieve control of Aortic Smooth Muscle Cell (AoSMC) attachment, elongation and alignment. The mechanical performances of these bundles were evaluated by elongation tests, related to the kind of biological functionalization and compared to non-functionalized samples. The alignment and differentiation of AoSMCs on single fibers and on the bundles were evaluated by microscopy and histochemical analyses.

UR - http://hdl.handle.net/10447/296866

UR - http://pubs.rsc.org/en/journals/journal/bm

M3 - Article

VL - 6

SP - 2518

EP - 2526

JO - Biomaterials Science

JF - Biomaterials Science

SN - 2047-4830

ER -