Multi-omics of Pseudoalteromonas haloplanktis TAC125: a quest for antimicrobial metabolic pathways

Fondi, M.; Perrin, E.; Fiore, A.; Diretto, G.; Maida, I.; Renzone, G.; Scaloni, A.; Barbara, C.; Carraro, L.; Fani, R.

Risultato della ricerca: Paper

Abstract

Backgrounds The Antarctic strain Pseudoalteromonas haloplanktis TAC125 is one of the model organisms of cold-adapted bacteria and is currently exploited as a new alternative expression host for numerous biotechnological applications. Interestingly, this bacterium has been reported to be able to inhibit the growth of Burkholderia cepacia complex (Bcc) strains, opportunistic pathogens responsible for the infection of immune-compromised patients. Most likely, this occurs through the synthesis of several different compounds, including Volatile Organic Compounds (VOCs), whose nature and characteristics are currently mostly unknown. Objectives To obtain a complete picture of cellular processes differentially regulated and associated with the capability of inhibiting Bcc growth. Methods Transcriptomic, proteomic and metabolomic experiments were carried out on P. haloplanktis TAC125 grown using two different cultivation media in which the strain is able to inhibit or not Bcc growth. Conclusions Bcc growth inhibition capability is deeply linked to the medium used to cultivate P. haloplanktis TAC125. Therefore, multi-omic data integration was used in order to explain the emergence of P. haloplanktis TAC125 phenotypes and, specifically, of cellular functional states associated to capability of inhibiting Bcc growth. In perspectives, the design of more focused strategies for a rational biotechnological exploitation of this strain will be boosted by the approach used and the results obtained in this work.
Lingua originaleEnglish
Stato di pubblicazionePublished - 2017

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Pseudoalteromonas
Burkholderia cepacia complex
Metabolic Networks and Pathways
Growth
Bacteria
Volatile Organic Compounds
Metabolomics
Proteomics
Phenotype
Infection

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Fondi, M.; Perrin, E.; Fiore, A.; Diretto, G.; Maida, I.; Renzone, G.; Scaloni, A.; Barbara, C.; Carraro, L.; Fani, R. (2017). Multi-omics of Pseudoalteromonas haloplanktis TAC125: a quest for antimicrobial metabolic pathways.

Multi-omics of Pseudoalteromonas haloplanktis TAC125: a quest for antimicrobial metabolic pathways. / Fondi, M.; Perrin, E.; Fiore, A.; Diretto, G.; Maida, I.; Renzone, G.; Scaloni, A.; Barbara, C.; Carraro, L.; Fani, R.

2017.

Risultato della ricerca: Paper

Fondi, M.; Perrin, E.; Fiore, A.; Diretto, G.; Maida, I.; Renzone, G.; Scaloni, A.; Barbara, C.; Carraro, L.; Fani, R. 2017, 'Multi-omics of Pseudoalteromonas haloplanktis TAC125: a quest for antimicrobial metabolic pathways'.
Fondi, M.; Perrin, E.; Fiore, A.; Diretto, G.; Maida, I.; Renzone, G.; Scaloni, A.; Barbara, C.; Carraro, L.; Fani, R.. Multi-omics of Pseudoalteromonas haloplanktis TAC125: a quest for antimicrobial metabolic pathways. 2017.
Fondi, M.; Perrin, E.; Fiore, A.; Diretto, G.; Maida, I.; Renzone, G.; Scaloni, A.; Barbara, C.; Carraro, L.; Fani, R. / Multi-omics of Pseudoalteromonas haloplanktis TAC125: a quest for antimicrobial metabolic pathways.
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title = "Multi-omics of Pseudoalteromonas haloplanktis TAC125: a quest for antimicrobial metabolic pathways",
abstract = "Backgrounds The Antarctic strain Pseudoalteromonas haloplanktis TAC125 is one of the model organisms of cold-adapted bacteria and is currently exploited as a new alternative expression host for numerous biotechnological applications. Interestingly, this bacterium has been reported to be able to inhibit the growth of Burkholderia cepacia complex (Bcc) strains, opportunistic pathogens responsible for the infection of immune-compromised patients. Most likely, this occurs through the synthesis of several different compounds, including Volatile Organic Compounds (VOCs), whose nature and characteristics are currently mostly unknown. Objectives To obtain a complete picture of cellular processes differentially regulated and associated with the capability of inhibiting Bcc growth. Methods Transcriptomic, proteomic and metabolomic experiments were carried out on P. haloplanktis TAC125 grown using two different cultivation media in which the strain is able to inhibit or not Bcc growth. Conclusions Bcc growth inhibition capability is deeply linked to the medium used to cultivate P. haloplanktis TAC125. Therefore, multi-omic data integration was used in order to explain the emergence of P. haloplanktis TAC125 phenotypes and, specifically, of cellular functional states associated to capability of inhibiting Bcc growth. In perspectives, the design of more focused strategies for a rational biotechnological exploitation of this strain will be boosted by the approach used and the results obtained in this work.",
author = "{Fondi, M.; Perrin, E.; Fiore, A.; Diretto, G.; Maida, I.; Renzone, G.; Scaloni, A.; Barbara, C.; Carraro, L.; Fani, R.} and Puglia, {Anna Maria} and Giuseppe Gallo and Alberto Sutera",
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T1 - Multi-omics of Pseudoalteromonas haloplanktis TAC125: a quest for antimicrobial metabolic pathways

AU - Fondi, M.; Perrin, E.; Fiore, A.; Diretto, G.; Maida, I.; Renzone, G.; Scaloni, A.; Barbara, C.; Carraro, L.; Fani, R.

AU - Puglia, Anna Maria

AU - Gallo, Giuseppe

AU - Sutera, Alberto

PY - 2017

Y1 - 2017

N2 - Backgrounds The Antarctic strain Pseudoalteromonas haloplanktis TAC125 is one of the model organisms of cold-adapted bacteria and is currently exploited as a new alternative expression host for numerous biotechnological applications. Interestingly, this bacterium has been reported to be able to inhibit the growth of Burkholderia cepacia complex (Bcc) strains, opportunistic pathogens responsible for the infection of immune-compromised patients. Most likely, this occurs through the synthesis of several different compounds, including Volatile Organic Compounds (VOCs), whose nature and characteristics are currently mostly unknown. Objectives To obtain a complete picture of cellular processes differentially regulated and associated with the capability of inhibiting Bcc growth. Methods Transcriptomic, proteomic and metabolomic experiments were carried out on P. haloplanktis TAC125 grown using two different cultivation media in which the strain is able to inhibit or not Bcc growth. Conclusions Bcc growth inhibition capability is deeply linked to the medium used to cultivate P. haloplanktis TAC125. Therefore, multi-omic data integration was used in order to explain the emergence of P. haloplanktis TAC125 phenotypes and, specifically, of cellular functional states associated to capability of inhibiting Bcc growth. In perspectives, the design of more focused strategies for a rational biotechnological exploitation of this strain will be boosted by the approach used and the results obtained in this work.

AB - Backgrounds The Antarctic strain Pseudoalteromonas haloplanktis TAC125 is one of the model organisms of cold-adapted bacteria and is currently exploited as a new alternative expression host for numerous biotechnological applications. Interestingly, this bacterium has been reported to be able to inhibit the growth of Burkholderia cepacia complex (Bcc) strains, opportunistic pathogens responsible for the infection of immune-compromised patients. Most likely, this occurs through the synthesis of several different compounds, including Volatile Organic Compounds (VOCs), whose nature and characteristics are currently mostly unknown. Objectives To obtain a complete picture of cellular processes differentially regulated and associated with the capability of inhibiting Bcc growth. Methods Transcriptomic, proteomic and metabolomic experiments were carried out on P. haloplanktis TAC125 grown using two different cultivation media in which the strain is able to inhibit or not Bcc growth. Conclusions Bcc growth inhibition capability is deeply linked to the medium used to cultivate P. haloplanktis TAC125. Therefore, multi-omic data integration was used in order to explain the emergence of P. haloplanktis TAC125 phenotypes and, specifically, of cellular functional states associated to capability of inhibiting Bcc growth. In perspectives, the design of more focused strategies for a rational biotechnological exploitation of this strain will be boosted by the approach used and the results obtained in this work.

UR - http://hdl.handle.net/10447/253644

M3 - Paper

ER -