Morphological, chemical and genetic diversity of wild myrtle (Myrtus communis L.) populations in Sicily.

Ignazio Cammalleri, Claudio Leto, Federico Martinelli, Teresa Tuttolomondo, Salvatore La Bella, Angela Fadda, Sara Melito, Federico Martinelli, Maria Giovanna Molinu, Maurizio Mulas

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Abstract

Myrtus communis L. is a shrub widespread in the Mediterranean area. The interest in this species is growing, mainly due to its pharmacological and aromatic properties. The overexploitation of wild populations induced increasing degradation of plant cover with serious risk of loss of genetic diversity. This research explored the morphological, chemical, and genetic diversity of wild myrtle populations in Sicily, with the aim to provide a first characterization of a core collection of 36 accessions from 7 localities for future domestication programs. Amplified fragment length polymorphism fingerprinting generated 152 polymorphic fragments. STRUCTURE analysis identified three genetic clusters (A, B, and C) corresponding to specific geographical origin. Analysis of molecular variance estimated a quite high overall fixation index (FST = 0.332). Misilmeri and Ispica were the more divergent populations (FST = 0.502), while M. Pellegrino and Scopello revealed the lowest FST (0.153). The relationships between genetic, morphological, and biometric data were investigated. Significant correlation between genetic clusters and bush shape/plant growth behavior was found (P < 0.005). Moreover, morphological traits such as leaf, fruit, and seed size were significantly correlated to Clusters B and C. Leaves’ secondary metabolite profiles were evaluated based on antioxidant activity and total tannin and phenol concentrations. High antioxidant activity differences were recorded using DPPH (21.4–35.5 mmol Trolox/100 g DW) and ABTS (24.2–39.5 mmol Trolox/100 g DW) methods. A low variability was observed among populations regarding phenol (2466–3800 mg catechin equivalents/100 g DW) and total tannin contents (93.9–262.3 mg catechin equivalents/100 g DW). Results indicated that multiple approaches based on genetic, morphological, and chemical traits might allow the characterization of natural myrtle diversity.
Lingua originaleEnglish
pagine (da-a)249-261
Numero di pagine13
RivistaTURKISH JOURNAL OF AGRICULTURE AND FORESTRY
Stato di pubblicazionePublished - 2016

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Myrtus
Sicily
Myrtus communis
genetic variation
Catechin
Tannins
tannin
Phenol
wild population
catechin
antioxidant
Population
phenol
tannins
Antioxidants
antioxidant activity
seed size
Cotyledon
biometry
domestication

All Science Journal Classification (ASJC) codes

  • Forestry
  • Food Science
  • Ecology

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@article{944d25b9e8b64d019f2ce6440fa2fcf0,
title = "Morphological, chemical and genetic diversity of wild myrtle (Myrtus communis L.) populations in Sicily.",
abstract = "Myrtus communis L. is a shrub widespread in the Mediterranean area. The interest in this species is growing, mainly due to its pharmacological and aromatic properties. The overexploitation of wild populations induced increasing degradation of plant cover with serious risk of loss of genetic diversity. This research explored the morphological, chemical, and genetic diversity of wild myrtle populations in Sicily, with the aim to provide a first characterization of a core collection of 36 accessions from 7 localities for future domestication programs. Amplified fragment length polymorphism fingerprinting generated 152 polymorphic fragments. STRUCTURE analysis identified three genetic clusters (A, B, and C) corresponding to specific geographical origin. Analysis of molecular variance estimated a quite high overall fixation index (FST = 0.332). Misilmeri and Ispica were the more divergent populations (FST = 0.502), while M. Pellegrino and Scopello revealed the lowest FST (0.153). The relationships between genetic, morphological, and biometric data were investigated. Significant correlation between genetic clusters and bush shape/plant growth behavior was found (P < 0.005). Moreover, morphological traits such as leaf, fruit, and seed size were significantly correlated to Clusters B and C. Leaves’ secondary metabolite profiles were evaluated based on antioxidant activity and total tannin and phenol concentrations. High antioxidant activity differences were recorded using DPPH (21.4–35.5 mmol Trolox/100 g DW) and ABTS (24.2–39.5 mmol Trolox/100 g DW) methods. A low variability was observed among populations regarding phenol (2466–3800 mg catechin equivalents/100 g DW) and total tannin contents (93.9–262.3 mg catechin equivalents/100 g DW). Results indicated that multiple approaches based on genetic, morphological, and chemical traits might allow the characterization of natural myrtle diversity.",
author = "Ignazio Cammalleri and Claudio Leto and Federico Martinelli and Teresa Tuttolomondo and {La Bella}, Salvatore and Angela Fadda and Sara Melito and Federico Martinelli and Molinu, {Maria Giovanna} and Maurizio Mulas",
year = "2016",
language = "English",
pages = "249--261",
journal = "Turk Tarim ve Ormancilik Dergisi/Turkish Journal of Agriculture and Forestry",
issn = "1300-011X",
publisher = "Turkiye Klinikleri Journal of Medical Sciences",

}

TY - JOUR

T1 - Morphological, chemical and genetic diversity of wild myrtle (Myrtus communis L.) populations in Sicily.

AU - Cammalleri, Ignazio

AU - Leto, Claudio

AU - Martinelli, Federico

AU - Tuttolomondo, Teresa

AU - La Bella, Salvatore

AU - Fadda, Angela

AU - Melito, Sara

AU - Martinelli, Federico

AU - Molinu, Maria Giovanna

AU - Mulas, Maurizio

PY - 2016

Y1 - 2016

N2 - Myrtus communis L. is a shrub widespread in the Mediterranean area. The interest in this species is growing, mainly due to its pharmacological and aromatic properties. The overexploitation of wild populations induced increasing degradation of plant cover with serious risk of loss of genetic diversity. This research explored the morphological, chemical, and genetic diversity of wild myrtle populations in Sicily, with the aim to provide a first characterization of a core collection of 36 accessions from 7 localities for future domestication programs. Amplified fragment length polymorphism fingerprinting generated 152 polymorphic fragments. STRUCTURE analysis identified three genetic clusters (A, B, and C) corresponding to specific geographical origin. Analysis of molecular variance estimated a quite high overall fixation index (FST = 0.332). Misilmeri and Ispica were the more divergent populations (FST = 0.502), while M. Pellegrino and Scopello revealed the lowest FST (0.153). The relationships between genetic, morphological, and biometric data were investigated. Significant correlation between genetic clusters and bush shape/plant growth behavior was found (P < 0.005). Moreover, morphological traits such as leaf, fruit, and seed size were significantly correlated to Clusters B and C. Leaves’ secondary metabolite profiles were evaluated based on antioxidant activity and total tannin and phenol concentrations. High antioxidant activity differences were recorded using DPPH (21.4–35.5 mmol Trolox/100 g DW) and ABTS (24.2–39.5 mmol Trolox/100 g DW) methods. A low variability was observed among populations regarding phenol (2466–3800 mg catechin equivalents/100 g DW) and total tannin contents (93.9–262.3 mg catechin equivalents/100 g DW). Results indicated that multiple approaches based on genetic, morphological, and chemical traits might allow the characterization of natural myrtle diversity.

AB - Myrtus communis L. is a shrub widespread in the Mediterranean area. The interest in this species is growing, mainly due to its pharmacological and aromatic properties. The overexploitation of wild populations induced increasing degradation of plant cover with serious risk of loss of genetic diversity. This research explored the morphological, chemical, and genetic diversity of wild myrtle populations in Sicily, with the aim to provide a first characterization of a core collection of 36 accessions from 7 localities for future domestication programs. Amplified fragment length polymorphism fingerprinting generated 152 polymorphic fragments. STRUCTURE analysis identified three genetic clusters (A, B, and C) corresponding to specific geographical origin. Analysis of molecular variance estimated a quite high overall fixation index (FST = 0.332). Misilmeri and Ispica were the more divergent populations (FST = 0.502), while M. Pellegrino and Scopello revealed the lowest FST (0.153). The relationships between genetic, morphological, and biometric data were investigated. Significant correlation between genetic clusters and bush shape/plant growth behavior was found (P < 0.005). Moreover, morphological traits such as leaf, fruit, and seed size were significantly correlated to Clusters B and C. Leaves’ secondary metabolite profiles were evaluated based on antioxidant activity and total tannin and phenol concentrations. High antioxidant activity differences were recorded using DPPH (21.4–35.5 mmol Trolox/100 g DW) and ABTS (24.2–39.5 mmol Trolox/100 g DW) methods. A low variability was observed among populations regarding phenol (2466–3800 mg catechin equivalents/100 g DW) and total tannin contents (93.9–262.3 mg catechin equivalents/100 g DW). Results indicated that multiple approaches based on genetic, morphological, and chemical traits might allow the characterization of natural myrtle diversity.

UR - http://hdl.handle.net/10447/202521

M3 - Article

SP - 249

EP - 261

JO - Turk Tarim ve Ormancilik Dergisi/Turkish Journal of Agriculture and Forestry

JF - Turk Tarim ve Ormancilik Dergisi/Turkish Journal of Agriculture and Forestry

SN - 1300-011X

ER -