The high grade ofphenotype plasticity of monocytes macrophages,is resumed in two different cell subsets namedM1 or M2. Several studies of microbial infections in vitro andin vivo, showed that, during the early stage of infection, macrophagesare polarized toward Ml phenotype that should beprotective against pathogen, while during the chronic phase ofinfection/disease macrophages polarize toward M2 phenotype toavoid damages from a prolonged Ml type activation.Obiettivo: In order to investigate if Mycobacterium tuberculosisinfection can drive circulating monocytes toward the expressionof Ml or M2 phenotypes, we have analyzed by flow cytometrymonocytes obtained from patients with active tuberculosis (TB)at early phase of disease and during anti mycobacterial therapy,subjects with latent TB and healthy uninfected control.Risultatil Analysis of surface markers expression showed noclearcut Ml/M2 polarization in all tested groups, but we founda very high percentage of RANK+ monocytes in patients withactive TB disease before treatment, while RANK expressionwas very faint in monocytes from all other experimental groups.Moreover the statistical analysis of geometric MFI of RANKshowed a signifìcant difference between patients with active diseasecompared to all the others groups.Conclusioni: Given that the available in vitro diagnostic testsare limited in discriminating subjects with latent or active disease,as well as response to therapy,,we speculate that the evaluationof RANK expression on monocytes could represent an additionalbiomarker useful to support diagnosis of active diseaseand monitor the response to therapy.
|Numero di pagine||1|
|Rivista||EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES|
|Stato di pubblicazione||Published - 2016|