Monitoring few molecular binding events in scalable confined aqueous compartments by raster image correlation spectroscopy (CADRICS)

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Abstract

The assembly of scalable liquid compartments for binding assays in array formats constitutes a topic of fundamental importance in life sciences. This challenge can be addressed by mimicking the structure of cellular compartments with biological native conditions. Here, inkjet printing is employed to develop up to hundreds of picoliter aqueous droplet arrays stabilized by oil-confinement with mild surfactants (Tween-20). The aqueous environments constitute specialized compartments in which biomolecules may exploit their function and a wide range of molecular interactions can be quantitatively investigated. Raster Image Correlation Spectroscopy (RICS) is employed to monitor in each compartment a restricted range of dynamic intermolecular events demonstrated through protein-binding assays involving the biotin/streptavidin model system.
Lingua originaleEnglish
pagine (da-a)4666-4676
Numero di pagine11
RivistaLab on a Chip
Volume16
Stato di pubblicazionePublished - 2016

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Assays
Spectroscopy
Streptavidin
Molecular interactions
Polysorbates
Monitoring
Biomolecules
Biotin
Surface-Active Agents
Printing
Oils
Liquids
Protein Binding

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering
  • Chemistry(all)
  • Biochemistry

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abstract = "The assembly of scalable liquid compartments for binding assays in array formats constitutes a topic of fundamental importance in life sciences. This challenge can be addressed by mimicking the structure of cellular compartments with biological native conditions. Here, inkjet printing is employed to develop up to hundreds of picoliter aqueous droplet arrays stabilized by oil-confinement with mild surfactants (Tween-20). The aqueous environments constitute specialized compartments in which biomolecules may exploit their function and a wide range of molecular interactions can be quantitatively investigated. Raster Image Correlation Spectroscopy (RICS) is employed to monitor in each compartment a restricted range of dynamic intermolecular events demonstrated through protein-binding assays involving the biotin/streptavidin model system.",
keywords = "droplets microarrays, inkjet printing, Raster Image Correlation Spectroscopy, water-in-oil emulsion, Streptvidin",
author = "Maurizio Leone and Pignataro, {Bruno Giuseppe} and Valeria Vetri and Vincenza Montalbano and Arrabito, {Giuseppe Domenico} and Felicia Cavaleri",
year = "2016",
language = "English",
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journal = "Lab on a Chip",
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publisher = "Royal Society of Chemistry",

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T1 - Monitoring few molecular binding events in scalable confined aqueous compartments by raster image correlation spectroscopy (CADRICS)

AU - Leone, Maurizio

AU - Pignataro, Bruno Giuseppe

AU - Vetri, Valeria

AU - Montalbano, Vincenza

AU - Arrabito, Giuseppe Domenico

AU - Cavaleri, Felicia

PY - 2016

Y1 - 2016

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AB - The assembly of scalable liquid compartments for binding assays in array formats constitutes a topic of fundamental importance in life sciences. This challenge can be addressed by mimicking the structure of cellular compartments with biological native conditions. Here, inkjet printing is employed to develop up to hundreds of picoliter aqueous droplet arrays stabilized by oil-confinement with mild surfactants (Tween-20). The aqueous environments constitute specialized compartments in which biomolecules may exploit their function and a wide range of molecular interactions can be quantitatively investigated. Raster Image Correlation Spectroscopy (RICS) is employed to monitor in each compartment a restricted range of dynamic intermolecular events demonstrated through protein-binding assays involving the biotin/streptavidin model system.

KW - droplets microarrays, inkjet printing, Raster Image Correlation Spectroscopy, water-in-oil emulsion, Streptvidin

UR - http://hdl.handle.net/10447/227812

M3 - Article

VL - 16

SP - 4666

EP - 4676

JO - Lab on a Chip

JF - Lab on a Chip

SN - 1473-0197

ER -