Monitoring few molecular binding events in scalable confined aqueous compartments by raster image correlation spectroscopy (CADRICS)

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Abstract

The assembly of scalable liquid compartments for binding assays in array formats constitutes a topic of fundamental importance in life sciences. This challenge can be addressed by mimicking the structure of cellular compartments with biological native conditions. Here, inkjet printing is employed to develop up to hundreds of picoliter aqueous droplet arrays stabilized by oil-confinement with mild surfactants (Tween-20). The aqueous environments constitute specialized compartments in which biomolecules may exploit their function and a wide range of molecular interactions can be quantitatively investigated. Raster Image Correlation Spectroscopy (RICS) is employed to monitor in each compartment a restricted range of dynamic intermolecular events demonstrated through protein-binding assays involving the biotin/streptavidin model system.
Lingua originaleEnglish
pagine (da-a)4666-4676
Numero di pagine11
RivistaLAB ON A CHIP
Volume16
Stato di pubblicazionePublished - 2016

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Assays
Spectroscopy
Streptavidin
Molecular interactions
Polysorbates
Monitoring
Biomolecules
Biotin
Surface-Active Agents
Printing
Oils
Liquids
Protein Binding

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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abstract = "The assembly of scalable liquid compartments for binding assays in array formats constitutes a topic of fundamental importance in life sciences. This challenge can be addressed by mimicking the structure of cellular compartments with biological native conditions. Here, inkjet printing is employed to develop up to hundreds of picoliter aqueous droplet arrays stabilized by oil-confinement with mild surfactants (Tween-20). The aqueous environments constitute specialized compartments in which biomolecules may exploit their function and a wide range of molecular interactions can be quantitatively investigated. Raster Image Correlation Spectroscopy (RICS) is employed to monitor in each compartment a restricted range of dynamic intermolecular events demonstrated through protein-binding assays involving the biotin/streptavidin model system.",
keywords = "Raster Image Correlation Spectroscopy, Streptvidin, droplets microarrays, inkjet printing, water-in-oil emulsion",
author = "Maurizio Leone and Valeria Vetri and Pignataro, {Bruno Giuseppe} and Arrabito, {Giuseppe Domenico} and Felicia Cavaleri and Vincenza Montalbano",
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T1 - Monitoring few molecular binding events in scalable confined aqueous compartments by raster image correlation spectroscopy (CADRICS)

AU - Leone, Maurizio

AU - Vetri, Valeria

AU - Pignataro, Bruno Giuseppe

AU - Arrabito, Giuseppe Domenico

AU - Cavaleri, Felicia

AU - Montalbano, Vincenza

PY - 2016

Y1 - 2016

N2 - The assembly of scalable liquid compartments for binding assays in array formats constitutes a topic of fundamental importance in life sciences. This challenge can be addressed by mimicking the structure of cellular compartments with biological native conditions. Here, inkjet printing is employed to develop up to hundreds of picoliter aqueous droplet arrays stabilized by oil-confinement with mild surfactants (Tween-20). The aqueous environments constitute specialized compartments in which biomolecules may exploit their function and a wide range of molecular interactions can be quantitatively investigated. Raster Image Correlation Spectroscopy (RICS) is employed to monitor in each compartment a restricted range of dynamic intermolecular events demonstrated through protein-binding assays involving the biotin/streptavidin model system.

AB - The assembly of scalable liquid compartments for binding assays in array formats constitutes a topic of fundamental importance in life sciences. This challenge can be addressed by mimicking the structure of cellular compartments with biological native conditions. Here, inkjet printing is employed to develop up to hundreds of picoliter aqueous droplet arrays stabilized by oil-confinement with mild surfactants (Tween-20). The aqueous environments constitute specialized compartments in which biomolecules may exploit their function and a wide range of molecular interactions can be quantitatively investigated. Raster Image Correlation Spectroscopy (RICS) is employed to monitor in each compartment a restricted range of dynamic intermolecular events demonstrated through protein-binding assays involving the biotin/streptavidin model system.

KW - Raster Image Correlation Spectroscopy

KW - Streptvidin

KW - droplets microarrays

KW - inkjet printing

KW - water-in-oil emulsion

UR - http://hdl.handle.net/10447/227812

M3 - Article

VL - 16

SP - 4666

EP - 4676

JO - LAB ON A CHIP

JF - LAB ON A CHIP

SN - 1473-0197

ER -