Molecular evolutionary analysis of type-1 human astroviruses identifies putative sites under selection pressure on the capsid protein

Simona De Grazia, Giovanni Giammanco, Gianvito Lanave, Vito Martella, Akos Gellért, Krisztian Banyai, Floriana Bonura, Noemi Urone, Vincenzo Cappa

Risultato della ricerca: Article

Abstract

Human astroviruses (HAstV) are important enteric pathogens that can be classified into eight sero/genotypes (HAstV-1 to -8). Although the various HAstV types show global spread, type-1 strains tend to be predominant. Molecular analysis of the genomic region encoding the capsid protein (ORF2) has revealed discrete sequence variation, with different lineages within each HAstV type and at least three major lineages have been identified within HAstV-1. Longitudinal epidemiological surveillance has revealed temporal shift of the various HAstV-1 lineages. Metadata analysis of HAstV-1 sequences available in the databases also revealed temporal shifts of the circulation of HAstV-1 lineages, suggesting possible antigenic-related mechanisms of selection at the sub-genotype level. By comparison of HAstV-1 capsid sequences, lineage-defining residues under positive selection were identified. Structural analysis of HAstV-1 capsid allowed identifying at least six residues exposed on the virion surface. Two residues were located in the VP34 (shell region) whilst four residues were mapped in the VP25/27 (protruding region) of HAstV capsid protein, in proximity of the putative receptor binding S site. These findings suggest that mechanisms similar to those observed and/or hypothesized for other enteric viruses are also shaping the evolution of HAstVs, with intra-typic diversification being a possible mechanism to decrease the antigenic pressure to which these viruses are exposed.
Lingua originaleEnglish
pagine (da-a)199-208
Numero di pagine10
RivistaINFECTION GENETICS AND EVOLUTION
Volume58
Stato di pubblicazionePublished - 2018

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Human astrovirus
Mamastrovirus
Capsid Proteins
coat proteins
Pressure
protein
virus
genotype
metadata
molecular analysis
structural analysis
genomics
pathogen
shell
capsid
Capsid
analysis
selection pressure
Genotype
Enterovirus

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

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Molecular evolutionary analysis of type-1 human astroviruses identifies putative sites under selection pressure on the capsid protein. / De Grazia, Simona; Giammanco, Giovanni; Lanave, Gianvito; Martella, Vito; Gellért, Akos; Banyai, Krisztian; Bonura, Floriana; Urone, Noemi; Cappa, Vincenzo.

In: INFECTION GENETICS AND EVOLUTION, Vol. 58, 2018, pag. 199-208.

Risultato della ricerca: Article

De Grazia, Simona ; Giammanco, Giovanni ; Lanave, Gianvito ; Martella, Vito ; Gellért, Akos ; Banyai, Krisztian ; Bonura, Floriana ; Urone, Noemi ; Cappa, Vincenzo. / Molecular evolutionary analysis of type-1 human astroviruses identifies putative sites under selection pressure on the capsid protein. In: INFECTION GENETICS AND EVOLUTION. 2018 ; Vol. 58. pagg. 199-208.
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abstract = "Human astroviruses (HAstV) are important enteric pathogens that can be classified into eight sero/genotypes (HAstV-1 to -8). Although the various HAstV types show global spread, type-1 strains tend to be predominant. Molecular analysis of the genomic region encoding the capsid protein (ORF2) has revealed discrete sequence variation, with different lineages within each HAstV type and at least three major lineages have been identified within HAstV-1. Longitudinal epidemiological surveillance has revealed temporal shift of the various HAstV-1 lineages. Metadata analysis of HAstV-1 sequences available in the databases also revealed temporal shifts of the circulation of HAstV-1 lineages, suggesting possible antigenic-related mechanisms of selection at the sub-genotype level. By comparison of HAstV-1 capsid sequences, lineage-defining residues under positive selection were identified. Structural analysis of HAstV-1 capsid allowed identifying at least six residues exposed on the virion surface. Two residues were located in the VP34 (shell region) whilst four residues were mapped in the VP25/27 (protruding region) of HAstV capsid protein, in proximity of the putative receptor binding S site. These findings suggest that mechanisms similar to those observed and/or hypothesized for other enteric viruses are also shaping the evolution of HAstVs, with intra-typic diversification being a possible mechanism to decrease the antigenic pressure to which these viruses are exposed.",
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AU - De Grazia, Simona

AU - Giammanco, Giovanni

AU - Lanave, Gianvito

AU - Martella, Vito

AU - Gellért, Akos

AU - Banyai, Krisztian

AU - Bonura, Floriana

AU - Urone, Noemi

AU - Cappa, Vincenzo

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