Molecular characterization and Phylogenetic analysis of Turnip mosaic virus (TuMV) in Erysimum linifolium L. in Italy

Risultato della ricerca: Articlepeer review

Abstract

In the Summer of 2012, Erysimum linifolium L. pot plants produced at an ornamental grower in Liguriaregion (northern Italy), showed unusual virus-like disease of dark mottle and stripes on mauve-purplepetals. A virus was mechanically transmitted from symptomatic flowers to several test plant speciesbelonging to Chenopodiaceae and Brassicaceae families. This virus was identified as an isolated ofturnip mosaic virus (TuMV) by PAS-ELISA analysis, electron microscopy negatively stained crudextracts and immuno-electron microscopy (IEM) tests. In the naturally infected E. linifolium plants,TuMV occurred alone, since any other viruses either by electron microscopy or mechanicalinoculations were detected. By applying RT-PCR a fragment of 862 bp was amplified corresponding toall coat protein (CP). The comparison of CP gene showed no correlations between their geneticvariation and geographical origins. The diversity in southern Europe appeared very low, most likely dueto the rapid growth of TuMV in relation to trade between different Countries. The consequent exchangeof infected propagation material shows that some lineages are adapted to particular crop species, andthat recombination is a significant generator of the genetic diversity in populations of this virus. This isthe first report of TuMV in E. linifolium worldwide.
Lingua originaleEnglish
pagine (da-a)97-102
Numero di pagine6
RivistaINTERNATIONAL RESEARCH JOURNAL OF PLANT SCIENCE
Volume4
Stato di pubblicazionePublished - 2013

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