The aim of this work has been to apply and improve the efficiency of moleculartools for the identification of Cyperus sp. from difTerent sources. Total DNA was extractedfrom fresh, degraded and ancient specimens. DNA molecules were the template for in vitroamplification of specific target regions referred to rcbL plastid gene (ribulose-l,5 -biphosphatecarboxylase/oxygenase large subunit). Dedicated DNA extraction and amplificationprotocols have been applied specifically for fresh/degraded and ancient samples. Particularly,specific set of primer, annealing temperatures and reaction mixtures have been performed.The PCR products, single DNA fragments, were resolved on agarose gel electrophoresisand sequenced. The sequences were compared by dedicated software and this allowed us toobtain genetic information.
|Numero di pagine||4|
|Stato di pubblicazione||Published - 2013|