Molecular analysis of Phytophthora diversity in nursery-grown ornamental and fruit plants.

Maria Isabella Prigigallo, Saveria Mosca, Prigigallo, Cooke, Leonardo Schena, Santa Olga Cacciola, Cooke, Saveria Mosca

Risultato della ricerca: Article

25 Citazioni (Scopus)

Abstract

The genetic diversity of Phytophthora spp. was investigated in potted ornamental and fruit tree species. A metabarcodingapproach was used, based on a semi-nested PCR with Phytophthora genus-specific primers targeting theITS1 region of the rDNA. More than 50 ITS1 sequence types representing at least 15 distinct Phytophthora taxa weredetected. Nine had ITS sequences that grouped them in defined taxonomic groups (P. nicotianae, P. citrophthora, P.meadii, P. taxon Pgchlamydo, P. cinnamomi, P. parvispora, P. cambivora, P. niederhauserii and P. lateralis) whereasthree phylotypes were associated to two or more taxa (P. citricola taxon E or III; P. pseudosyringae, P. ilicis or P.nemorosa; and P. cryptogea, P. erythroseptica, P. himalayensis or P. sp. ‘kelmania’) that can be challenging to resolvewith ITS1 sequences alone. Three additional phylotypes were considered as representatives of novel Phytophthora taxaand defined as P. meadii-like, P. cinnamomi-like and P. niederhauserii-like. Furthermore, the analyses highlighted avery complex assemblage of Phytophthora taxa in ornamental nurseries within a limited geographic area and providedsome indications of structure amongst populations of P. nicotianae (the most prevalent taxon) and other taxa. Datarevealed new host–pathogen combinations, evidence of new species previously unreported in Italy (P. lateralis) or Europe(P. meadii) and phylotypes representative of species that remain to be taxonomically defined. Furthermore, theresults reinforced the primary role of plant nurseries in favouring the introduction, dissemination and evolution ofPhytophthora species.
Lingua originaleEnglish
pagine (da-a)1308-1319
Numero di pagine12
RivistaPlant Pathology
Volume64
Stato di pubblicazionePublished - 2015

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Phytophthora
Nurseries
Fruit
fruits
Phytophthora nicotianae
Phytophthora meadii
Phytophthora citrophthora
plant nurseries
ornamental trees
Phytophthora cinnamomi
fruit trees
Ribosomal DNA
Italy
new combination
population structure
Polymerase Chain Reaction
genetic variation
new species
Population
phylotype

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture

Cita questo

Prigigallo, M. I., Mosca, S., Prigigallo, Cooke, Schena, L., Cacciola, S. O., ... Mosca, S. (2015). Molecular analysis of Phytophthora diversity in nursery-grown ornamental and fruit plants. Plant Pathology, 64, 1308-1319.

Molecular analysis of Phytophthora diversity in nursery-grown ornamental and fruit plants. / Prigigallo, Maria Isabella; Mosca, Saveria; Prigigallo; Cooke; Schena, Leonardo; Cacciola, Santa Olga; Cooke; Mosca, Saveria.

In: Plant Pathology, Vol. 64, 2015, pag. 1308-1319.

Risultato della ricerca: Article

Prigigallo, MI, Mosca, S, Prigigallo, Cooke, Schena, L, Cacciola, SO, Cooke & Mosca, S 2015, 'Molecular analysis of Phytophthora diversity in nursery-grown ornamental and fruit plants.', Plant Pathology, vol. 64, pagg. 1308-1319.
Prigigallo MI, Mosca S, Prigigallo, Cooke, Schena L, Cacciola SO e altri. Molecular analysis of Phytophthora diversity in nursery-grown ornamental and fruit plants. Plant Pathology. 2015;64:1308-1319.
Prigigallo, Maria Isabella ; Mosca, Saveria ; Prigigallo ; Cooke ; Schena, Leonardo ; Cacciola, Santa Olga ; Cooke ; Mosca, Saveria. / Molecular analysis of Phytophthora diversity in nursery-grown ornamental and fruit plants. In: Plant Pathology. 2015 ; Vol. 64. pagg. 1308-1319.
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abstract = "The genetic diversity of Phytophthora spp. was investigated in potted ornamental and fruit tree species. A metabarcodingapproach was used, based on a semi-nested PCR with Phytophthora genus-specific primers targeting theITS1 region of the rDNA. More than 50 ITS1 sequence types representing at least 15 distinct Phytophthora taxa weredetected. Nine had ITS sequences that grouped them in defined taxonomic groups (P. nicotianae, P. citrophthora, P.meadii, P. taxon Pgchlamydo, P. cinnamomi, P. parvispora, P. cambivora, P. niederhauserii and P. lateralis) whereasthree phylotypes were associated to two or more taxa (P. citricola taxon E or III; P. pseudosyringae, P. ilicis or P.nemorosa; and P. cryptogea, P. erythroseptica, P. himalayensis or P. sp. ‘kelmania’) that can be challenging to resolvewith ITS1 sequences alone. Three additional phylotypes were considered as representatives of novel Phytophthora taxaand defined as P. meadii-like, P. cinnamomi-like and P. niederhauserii-like. Furthermore, the analyses highlighted avery complex assemblage of Phytophthora taxa in ornamental nurseries within a limited geographic area and providedsome indications of structure amongst populations of P. nicotianae (the most prevalent taxon) and other taxa. Datarevealed new host–pathogen combinations, evidence of new species previously unreported in Italy (P. lateralis) or Europe(P. meadii) and phylotypes representative of species that remain to be taxonomically defined. Furthermore, theresults reinforced the primary role of plant nurseries in favouring the introduction, dissemination and evolution ofPhytophthora species.",
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AU - Mosca, Saveria

AU - Prigigallo, null

AU - Cooke, null

AU - Schena, Leonardo

AU - Cacciola, Santa Olga

AU - Cooke, null

AU - Mosca, Saveria

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N2 - The genetic diversity of Phytophthora spp. was investigated in potted ornamental and fruit tree species. A metabarcodingapproach was used, based on a semi-nested PCR with Phytophthora genus-specific primers targeting theITS1 region of the rDNA. More than 50 ITS1 sequence types representing at least 15 distinct Phytophthora taxa weredetected. Nine had ITS sequences that grouped them in defined taxonomic groups (P. nicotianae, P. citrophthora, P.meadii, P. taxon Pgchlamydo, P. cinnamomi, P. parvispora, P. cambivora, P. niederhauserii and P. lateralis) whereasthree phylotypes were associated to two or more taxa (P. citricola taxon E or III; P. pseudosyringae, P. ilicis or P.nemorosa; and P. cryptogea, P. erythroseptica, P. himalayensis or P. sp. ‘kelmania’) that can be challenging to resolvewith ITS1 sequences alone. Three additional phylotypes were considered as representatives of novel Phytophthora taxaand defined as P. meadii-like, P. cinnamomi-like and P. niederhauserii-like. Furthermore, the analyses highlighted avery complex assemblage of Phytophthora taxa in ornamental nurseries within a limited geographic area and providedsome indications of structure amongst populations of P. nicotianae (the most prevalent taxon) and other taxa. Datarevealed new host–pathogen combinations, evidence of new species previously unreported in Italy (P. lateralis) or Europe(P. meadii) and phylotypes representative of species that remain to be taxonomically defined. Furthermore, theresults reinforced the primary role of plant nurseries in favouring the introduction, dissemination and evolution ofPhytophthora species.

AB - The genetic diversity of Phytophthora spp. was investigated in potted ornamental and fruit tree species. A metabarcodingapproach was used, based on a semi-nested PCR with Phytophthora genus-specific primers targeting theITS1 region of the rDNA. More than 50 ITS1 sequence types representing at least 15 distinct Phytophthora taxa weredetected. Nine had ITS sequences that grouped them in defined taxonomic groups (P. nicotianae, P. citrophthora, P.meadii, P. taxon Pgchlamydo, P. cinnamomi, P. parvispora, P. cambivora, P. niederhauserii and P. lateralis) whereasthree phylotypes were associated to two or more taxa (P. citricola taxon E or III; P. pseudosyringae, P. ilicis or P.nemorosa; and P. cryptogea, P. erythroseptica, P. himalayensis or P. sp. ‘kelmania’) that can be challenging to resolvewith ITS1 sequences alone. Three additional phylotypes were considered as representatives of novel Phytophthora taxaand defined as P. meadii-like, P. cinnamomi-like and P. niederhauserii-like. Furthermore, the analyses highlighted avery complex assemblage of Phytophthora taxa in ornamental nurseries within a limited geographic area and providedsome indications of structure amongst populations of P. nicotianae (the most prevalent taxon) and other taxa. Datarevealed new host–pathogen combinations, evidence of new species previously unreported in Italy (P. lateralis) or Europe(P. meadii) and phylotypes representative of species that remain to be taxonomically defined. Furthermore, theresults reinforced the primary role of plant nurseries in favouring the introduction, dissemination and evolution ofPhytophthora species.

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JF - Plant Pathology

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