Molecular alterations in spermatozoa of a family case living in the land of fires. A first look at possible transgenerational effects of pollutants

Liana Bosco, Francesco Paolo Marra, Marina Prisco, Antonella Giarra, Tiziana Notari, Luigi Montano, Gennaro Lettieri, Federica Marra, Claudia Moriello, Marco Trifuoggi, Marina Piscopo

Risultato della ricerca: Articlepeer review

2 Citazioni (Scopus)

Abstract

In our previous work, we reported alterations in protamines/histones ratio, in DNA binding of these proteins and their involvement in DNA oxidative damage in 84% of the young men living in the Land of Fires. In the present work, we extended our findings, evaluating any alterations in spermatozoa of a family case, a father and son, living in this area, to also give a first look at the possibility of transgenerational inherited effects of environmental contaminants on the molecular alterations of sperm nuclear basic proteins (SNBP), DNA and semen parameters. In the father and son, we found a diverse excess of copper and chromium in the semen, different alterations in SNBP content and low DNA binding affinity of these proteins. In addition, DNA damage, in the presence of CuCl2 and H2 O2, increased by adding both the father and son SNBP. Interestingly, son SNBP, unlike his father, showed an unstable DNA binding and were able to produce DNA damage even without external addition of CuCl2, in line with a lower seminal antioxidant activity than the father. The peculiarity of some characteristics of son semen could be a basis for possible future studies on transgenerational effects of pollutants on fertility.
Lingua originaleEnglish
pagine (da-a)1-18
Numero di pagine18
RivistaInternational Journal of Molecular Sciences
Volume21
Stato di pubblicazionePublished - 2020

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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