Modeling interactions between Human EquilibrativeNucleoside Transporter-1 and other factors involved in the response togemcitabine treatment to predict clinical outcomes in pancreatic ductaladenocarcinoma patients.

Francesca Rappa, Francesco Cappello, Valerio Pazienza, Lucia Lombardi, Massimiliano Copetti, Pierluigi Di Sebastiano, Fabio F. Di Mola, Andrea Fontana, Francesca Tavano, Francesca P. Burbaci, Francesca Rappa, Manlio Vinciguerra, Paolo Graziano, Evaristo Maiello, Angelo Andriulli, Francesco Cappello, Fabio Pellegrini

Risultato della ricerca: Articlepeer review

8 Citazioni (Scopus)

Abstract

BackgroundPancreatic ductal adenocarcinoma (PDAC) is an extremely aggressive malignancy, characterized by largely unsatisfactory responses to the currently available therapeutic strategies. In this study we evaluated the expression of genes involved in gemcitabine uptake in a selected cohort of patients with PDAC, with well-defined clinical-pathological features.MethodsmRNA levels of hENT1, CHOP, MRP1 and DCK were evaluated by means of qRT-PCR in matched pairs of tumor and adjacent normal tissue samples collected from PDAC patients treated with gemcitabine after surgical tumor resection. To detect possible interaction between gene expression levels and to identify subgroups of patients at different mortality/progression risk, the RECursive Partitioning and Amalgamation (RECPAM) method was used.ResultsRECPAM analysis showed that DCK and CHOP were most relevant variables for the identification of patients with different mortality risk, while hENT1 and CHOP were able to identify subgroups of patients with different disease progression risk. Conclusion: hENT1, CHOP, MRP1 and DCK appear correlated to PDAC, and this interaction might influence disease behavior.
Lingua originaleEnglish
Numero di pagine20
RivistaJournal of Translational Medicine
Volume12
Stato di pubblicazionePublished - 2014

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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