Mitochondrial protein expression in Ratt and in human cells

Risultato della ricerca: Article

3 Citazioni (Scopus)

Abstract

In the present paper, we analyzed some aspects of the post-transcriptional regulation of two COX (cy-tochrome c oxidase) subunits, i.e. mitochondrion-encoded COXIII and nucleus-encoded COXIV. In particular, by T1 RNase protection assays, we found two proteins, present in mitochondrial extracts from adult rat brain and testis, able to bind COXIII mRNA. We also found cytoplasmic proteins present in testis, kidney and heart extracts that bind COXIV mRNA. Moreover, to study the expression of mitochondrial proteins in tumor cells, we quantitated COXIV and Hsp60 chaperonin abundance in two epithelial cell lines from human breast, one neoplastic (MDA-MB231) and the other immortalized and non-tumoral (HB2). The results obtained showed that COXIV amount was higher in tumor cells than in immortalized cells, while Hsp60 was more abundant in HB2 cell line.
Lingua originaleEnglish
pagine (da-a)371-374
Numero di pagine4
RivistaCaryologia
Volume59
Stato di pubblicazionePublished - 2006

All Science Journal Classification (ASJC) codes

  • Genetics
  • Agricultural and Biological Sciences(all)

Cita questo

Mitochondrial protein expression in Ratt and in human cells. /.

In: Caryologia, Vol. 59, 2006, pag. 371-374.

Risultato della ricerca: Article

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title = "Mitochondrial protein expression in Ratt and in human cells",
abstract = "In the present paper, we analyzed some aspects of the post-transcriptional regulation of two COX (cy-tochrome c oxidase) subunits, i.e. mitochondrion-encoded COXIII and nucleus-encoded COXIV. In particular, by T1 RNase protection assays, we found two proteins, present in mitochondrial extracts from adult rat brain and testis, able to bind COXIII mRNA. We also found cytoplasmic proteins present in testis, kidney and heart extracts that bind COXIV mRNA. Moreover, to study the expression of mitochondrial proteins in tumor cells, we quantitated COXIV and Hsp60 chaperonin abundance in two epithelial cell lines from human breast, one neoplastic (MDA-MB231) and the other immortalized and non-tumoral (HB2). The results obtained showed that COXIV amount was higher in tumor cells than in immortalized cells, while Hsp60 was more abundant in HB2 cell line.",
author = "Claudio Luparello and Giuseppe Cannino and {Di Liegro}, {Carlo Maria}",
year = "2006",
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journal = "Caryologia",
issn = "0008-7114",
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T1 - Mitochondrial protein expression in Ratt and in human cells

AU - Luparello, Claudio

AU - Cannino, Giuseppe

AU - Di Liegro, Carlo Maria

PY - 2006

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AB - In the present paper, we analyzed some aspects of the post-transcriptional regulation of two COX (cy-tochrome c oxidase) subunits, i.e. mitochondrion-encoded COXIII and nucleus-encoded COXIV. In particular, by T1 RNase protection assays, we found two proteins, present in mitochondrial extracts from adult rat brain and testis, able to bind COXIII mRNA. We also found cytoplasmic proteins present in testis, kidney and heart extracts that bind COXIV mRNA. Moreover, to study the expression of mitochondrial proteins in tumor cells, we quantitated COXIV and Hsp60 chaperonin abundance in two epithelial cell lines from human breast, one neoplastic (MDA-MB231) and the other immortalized and non-tumoral (HB2). The results obtained showed that COXIV amount was higher in tumor cells than in immortalized cells, while Hsp60 was more abundant in HB2 cell line.

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EP - 374

JO - Caryologia

JF - Caryologia

SN - 0008-7114

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