Mir-31-5p is a lipus-mechanosensitive microrna that targets hif-1α signaling and cytoskeletal proteins

Alice Conigliaro, Riccardo Alessandro, Stefania Setti, Daniele Bellavia, Francesca Salamanna, Angela De Luca, Valeria Carina, Viviana Costa, Riccardo Alessandro, Gianluca Giavaresi, Lavinia Raimondi, Milena Fini

Risultato della ricerca: Articlepeer review

6 Citazioni (Scopus)

Abstract

The roles of low-intensity pulsed ultrasound (LIPUS) and microRNAs (miRNAs) on hMSCs commitments have already been investigated; however, the effects of the application of their co-treatments in an in vitro cell model are still unknown. Our previous studies demonstrated that (i) LIPUS modulated hMSCs cytoskeletal organization and (ii) miRNA-675-5p have a role in HIF-1α signaling modulation during hMSCs osteoblast commitment. We investigated for the first time the role of LIPUS as promoter tool for miRNA expression. Thanks to bioinformatic analysis, we identified miR-31-5p as a LIPUS-induced miRNA and investigated its role through in vitro studies of gain and loss of function. Results highlighted that LIPUS stimulation induced a hypoxia adaptive cell response, which determines a reorganization of cell membrane and cytoskeleton proteins. MiR-31-5p gain and loss of function studies, demonstrated as miR-31-5p overexpression, were able to induce hypoxic and cytoskeletal responses. Moreover, the co-treatments LIPUS and miR-31-5p inhibitor abolished the hypoxic responses including angiogenesis and the expression of Rho family proteins. MiR-31-5p was identified as a LIPUS-mechanosensitive miRNAs and may be considered a new therapeutic option to promote or abolish hypoxic response and cytoskeletal organization on hMSCs during the bone regeneration process.
Lingua originaleEnglish
pagine (da-a)1569-1583
Numero di pagine15
RivistaInternational Journal of Molecular Sciences
Volume20
Stato di pubblicazionePublished - 2019

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.1600.1606???
  • ???subjectarea.asjc.1600.1605???
  • ???subjectarea.asjc.1600.1604???
  • ???subjectarea.asjc.1500.1503???
  • ???subjectarea.asjc.1300.1312???
  • ???subjectarea.asjc.1600.1607???

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