A scalable, single-step, synthetic approach for the manufacture ofbiocompatible, functionalized micro- and nanogels is presented. In particular,poly(N-vinyl pyrrolidone)-grafted-(aminopropyl)methacrylamide microgels andnanogels were generated through e-beam irradiation of PVP aqueous solutions inthe presence of a primary amino-group-carrying monomer. Particles withdifferent hydrodynamic diameters and surface charge densities were obtained atthe variance of the irradiation conditions. Chemical structure was investigated bydifferent spectroscopic techniques. Fluorescent variants were generated throughfluorescein isothiocyanate attachment to the primary amino groups grafted toPVP, to both quantify the available functional groups for bioconjugation andfollow nanogels localization in cell cultures. Finally, a model protein, bovineserum albumin, was conjugated to the nanogels to demonstrate the attachmentof biologically relevant molecules for targeting purposes in drug delivery. Thedescribed approach provides a novel strategy to fabricate biohybrid nanogelswith a very promising potential in nanomedicine.
|Numero di pagine||13|
|Stato di pubblicazione||Published - 2012|
All Science Journal Classification (ASJC) codes