Abstract

The brain capillary endothelial cells (BCECs) form the blood brain barrier (BBB) under the effects of the brainmicroenvironment. BCECs are sealed together by tight junctions (TJs) that are responsible for the barrier phenotype. Inthese junctions, molecules such as JAM (junctional adhesion molecules), occludin and claudins are present. Threedimensionalscaffolds are used to grow cells in order to obtain in vitro engineered tissues. On the base of theseconsiderations, the aim of this work was to understand whether the endothelial cells were able to grow and survive on anew three-dimensional structure. If yes, indeed, this system could be further enriched and used to set a three-dimensionalin vitro model of BBB containing also neurons and astrocytes. The starting work has been focusing on BCEC growth onpoly-L-lactate (PLLA) 3D scaffold. BCECs were seeded (50,000 cells/scaffold) on scaffolds prepared at 0°C and precoatedwith Collagen IV. TJ distribution was analyzed by immunofluorescence with zonula occludens 1 (ZO1) antibodiesand the number of alive cells was evaluated by staining cells with acridine orange (AO)/Ethidium bromide (Etbr).Moreover, scaffolds were fixed, sectioned and observed by transmission electron microscopy (TEM). Our datademonstrate that BCECs adapt well to this kind of scaffolds and are also able to migrate inside the pores present in them.
Lingua originaleEnglish
Titolo della pubblicazione ospiteMicroscopy and imaging science
Pagine260-264
Numero di pagine5
Stato di pubblicazionePublished - 2017

Serie di pubblicazioni

NomeMICROSCOPY BOOK SERIES

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Endothelial Cells
Tight Junctions
Brain
Blood-Brain Barrier
Junctional Adhesion Molecules
Claudins
Occludin
Acridine Orange
Ethidium
Transmission Electron Microscopy
Astrocytes
Fluorescent Antibody Technique
poly(lactic acid)
Lactic Acid
Collagen
Cell Count
Staining and Labeling
Phenotype
Neurons
Growth

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title = "Migration of brain capillary endothelial cells inside poly (lactic acid) 3D scaffolds",
abstract = "The brain capillary endothelial cells (BCECs) form the blood brain barrier (BBB) under the effects of the brainmicroenvironment. BCECs are sealed together by tight junctions (TJs) that are responsible for the barrier phenotype. Inthese junctions, molecules such as JAM (junctional adhesion molecules), occludin and claudins are present. Threedimensionalscaffolds are used to grow cells in order to obtain in vitro engineered tissues. On the base of theseconsiderations, the aim of this work was to understand whether the endothelial cells were able to grow and survive on anew three-dimensional structure. If yes, indeed, this system could be further enriched and used to set a three-dimensionalin vitro model of BBB containing also neurons and astrocytes. The starting work has been focusing on BCEC growth onpoly-L-lactate (PLLA) 3D scaffold. BCECs were seeded (50,000 cells/scaffold) on scaffolds prepared at 0°C and precoatedwith Collagen IV. TJ distribution was analyzed by immunofluorescence with zonula occludens 1 (ZO1) antibodiesand the number of alive cells was evaluated by staining cells with acridine orange (AO)/Ethidium bromide (Etbr).Moreover, scaffolds were fixed, sectioned and observed by transmission electron microscopy (TEM). Our datademonstrate that BCECs adapt well to this kind of scaffolds and are also able to migrate inside the pores present in them.",
author = "{Di Bella}, {Maria Antonietta} and Gabriella Schiera and Brucato, {Valerio Maria Bartolo} and {Carfi' Pavia}, Francesco and {Di Liegro}, Italia and Francesca Zummo",
year = "2017",
language = "English",
isbn = "978-84-942134-9-6",
series = "MICROSCOPY BOOK SERIES",
pages = "260--264",
booktitle = "Microscopy and imaging science",

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TY - CHAP

T1 - Migration of brain capillary endothelial cells inside poly (lactic acid) 3D scaffolds

AU - Di Bella, Maria Antonietta

AU - Schiera, Gabriella

AU - Brucato, Valerio Maria Bartolo

AU - Carfi' Pavia, Francesco

AU - Di Liegro, Italia

AU - Zummo, Francesca

PY - 2017

Y1 - 2017

N2 - The brain capillary endothelial cells (BCECs) form the blood brain barrier (BBB) under the effects of the brainmicroenvironment. BCECs are sealed together by tight junctions (TJs) that are responsible for the barrier phenotype. Inthese junctions, molecules such as JAM (junctional adhesion molecules), occludin and claudins are present. Threedimensionalscaffolds are used to grow cells in order to obtain in vitro engineered tissues. On the base of theseconsiderations, the aim of this work was to understand whether the endothelial cells were able to grow and survive on anew three-dimensional structure. If yes, indeed, this system could be further enriched and used to set a three-dimensionalin vitro model of BBB containing also neurons and astrocytes. The starting work has been focusing on BCEC growth onpoly-L-lactate (PLLA) 3D scaffold. BCECs were seeded (50,000 cells/scaffold) on scaffolds prepared at 0°C and precoatedwith Collagen IV. TJ distribution was analyzed by immunofluorescence with zonula occludens 1 (ZO1) antibodiesand the number of alive cells was evaluated by staining cells with acridine orange (AO)/Ethidium bromide (Etbr).Moreover, scaffolds were fixed, sectioned and observed by transmission electron microscopy (TEM). Our datademonstrate that BCECs adapt well to this kind of scaffolds and are also able to migrate inside the pores present in them.

AB - The brain capillary endothelial cells (BCECs) form the blood brain barrier (BBB) under the effects of the brainmicroenvironment. BCECs are sealed together by tight junctions (TJs) that are responsible for the barrier phenotype. Inthese junctions, molecules such as JAM (junctional adhesion molecules), occludin and claudins are present. Threedimensionalscaffolds are used to grow cells in order to obtain in vitro engineered tissues. On the base of theseconsiderations, the aim of this work was to understand whether the endothelial cells were able to grow and survive on anew three-dimensional structure. If yes, indeed, this system could be further enriched and used to set a three-dimensionalin vitro model of BBB containing also neurons and astrocytes. The starting work has been focusing on BCEC growth onpoly-L-lactate (PLLA) 3D scaffold. BCECs were seeded (50,000 cells/scaffold) on scaffolds prepared at 0°C and precoatedwith Collagen IV. TJ distribution was analyzed by immunofluorescence with zonula occludens 1 (ZO1) antibodiesand the number of alive cells was evaluated by staining cells with acridine orange (AO)/Ethidium bromide (Etbr).Moreover, scaffolds were fixed, sectioned and observed by transmission electron microscopy (TEM). Our datademonstrate that BCECs adapt well to this kind of scaffolds and are also able to migrate inside the pores present in them.

UR - http://hdl.handle.net/10447/234337

M3 - Chapter

SN - 978-84-942134-9-6

T3 - MICROSCOPY BOOK SERIES

SP - 260

EP - 264

BT - Microscopy and imaging science

ER -