Abstract

The brain capillary endothelial cells (BCECs) form the blood brain barrier (BBB) under the effects of the brainmicroenvironment. BCECs are sealed together by tight junctions (TJs) that are responsible for the barrier phenotype. Inthese junctions, molecules such as JAM (junctional adhesion molecules), occludin and claudins are present. Threedimensionalscaffolds are used to grow cells in order to obtain in vitro engineered tissues. On the base of theseconsiderations, the aim of this work was to understand whether the endothelial cells were able to grow and survive on anew three-dimensional structure. If yes, indeed, this system could be further enriched and used to set a three-dimensionalin vitro model of BBB containing also neurons and astrocytes. The starting work has been focusing on BCEC growth onpoly-L-lactate (PLLA) 3D scaffold. BCECs were seeded (50,000 cells/scaffold) on scaffolds prepared at 0°C and precoatedwith Collagen IV. TJ distribution was analyzed by immunofluorescence with zonula occludens 1 (ZO1) antibodiesand the number of alive cells was evaluated by staining cells with acridine orange (AO)/Ethidium bromide (Etbr).Moreover, scaffolds were fixed, sectioned and observed by transmission electron microscopy (TEM). Our datademonstrate that BCECs adapt well to this kind of scaffolds and are also able to migrate inside the pores present in them.
Lingua originaleEnglish
Titolo della pubblicazione ospiteMicroscopy and imaging science
Pagine260-264
Numero di pagine5
Stato di pubblicazionePublished - 2017

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