Abstract
Type V collagen is known to be over-deposited in the stroma of ductal infiltrating carcinomas of the breast. When used as a substrate, type V collagen restrains growth and invasion, and affects gene expression of 8701-BC ductal infiltrating carcinomas cells. Here we supplement existing data by demonstrating type V collagen dependent upregulation of capn2 gene expression in 8701-BC cells through differential display-PCR and Western blot assays. Furthermore, we suggest that our data obtained by centrifugal sedimentation and electrophoresis strongly suggest a correlation between calpain overproduction and DNA fragmentation, since the incubation with calpain inhibitor partly reverts the latter.
Lingua originale | English |
---|---|
pagine (da-a) | 457-465 |
Numero di pagine | 9 |
Rivista | Biological Chemistry |
Volume | 388 |
Stato di pubblicazione | Published - 2007 |
All Science Journal Classification (ASJC) codes
- ???subjectarea.asjc.1300.1303???
- ???subjectarea.asjc.1300.1312???
- ???subjectarea.asjc.1300.1308???