TY - CHAP
T1 - Microspore embryogenesis through anther culture in Citrus clementina Hort. ex Tan
AU - Germana', Maria
AU - Germanà, Maria Antonietta
AU - Chiancone, Benedetta
PY - 2016
Y1 - 2016
N2 - Anther culture is a biotechnological method that allows to obtain, in one step, homozygous plants, very important to plant breeding, due to their numerous applications in mutation research, selection, genome sequencing, genetic analysis, and transformation. To induce the microspores, i.e., the immature male gametes, to switch from the normal gametophytic pathway to the sporophytic one, it is necessary to submit them to a type of stress, such as high or low temperature, starvation, or magnetic field. Stress can be applied to the donor plants and/or the fl oral buds or the anthers or the isolated microspores, before or during the culture. In this chapter, the protocol to induce gametic embryogenesis from anther culture of several cultivars of Citrus clementina Hort. ex Tan. is reported.
AB - Anther culture is a biotechnological method that allows to obtain, in one step, homozygous plants, very important to plant breeding, due to their numerous applications in mutation research, selection, genome sequencing, genetic analysis, and transformation. To induce the microspores, i.e., the immature male gametes, to switch from the normal gametophytic pathway to the sporophytic one, it is necessary to submit them to a type of stress, such as high or low temperature, starvation, or magnetic field. Stress can be applied to the donor plants and/or the fl oral buds or the anthers or the isolated microspores, before or during the culture. In this chapter, the protocol to induce gametic embryogenesis from anther culture of several cultivars of Citrus clementina Hort. ex Tan. is reported.
UR - http://hdl.handle.net/10447/219613
UR - http://www.springer.com/series/7651
M3 - Chapter
T3 - METHODS IN MOLECULAR BIOLOGY
SP - 475
EP - 487
BT - IN VITRO EMBRYOGENESIS IN HIGHER PLANTS series "Methods in Molecular Biology"
ER -