Microspore embryogenesis induced through in vitro anther culture of almond (Prunus dulcis Mill.)

Cimò, G.; Germanà, M.

Risultato della ricerca: Article

4 Citazioni (Scopus)

Abstract

Anther culture is one of the most widely used methods to induce gametic embryogenesis. The aim of this investigation was to induce microspore embryogenesis in almond (Prunus dulcis Mill.), through this technique. Anthers were cultured at the vacuolated developmental stage, and seven cultivars, two culture media and two temperature treatments were assessed. Although evidence of the microspore induction was observed in all the genotypes and treatments tested (symmetrical nucleus division and multinucleated structures), calli were produced merely by anthers cultured in the medium P and the regeneration of embryos was detected only in anthers of the cultivars Filippo Ceo, Lauranne and Genco, placed on medium P and subjected to the Control treatment (direct culture at 25 ± 1 °C, without the hot thermal shock at 35 ± 1 °C for 7 days). Characterization by SSR marker analysis of the embryo genotypes revealed that the regenerants had a single allele for each locus whereas the parent cultivar was heterozygous, indicating their development from haploid microspores. This study reports the evidence of gametic embryogenesis and, particularly, of microspore embryogenesis through in vitro anther culture, in almond, and, for the first time to our knowledge, the production of homozygous embryos.
Lingua originaleEnglish
pagine (da-a)85-95
Numero di pagine11
RivistaPLANT CELL TISSUE AND ORGAN CULTURE
Volume128
Stato di pubblicazionePublished - 2017

Fingerprint

Prunus dulcis
anther culture
microspores
almonds
embryogenesis
anthers
embryo (animal)
cultivars
genotype
haploidy
heat stress
callus
culture media
developmental stages
alleles
loci
methodology
temperature

All Science Journal Classification (ASJC) codes

  • Horticulture

Cita questo

Microspore embryogenesis induced through in vitro anther culture of almond (Prunus dulcis Mill.). / Cimò, G.; Germanà, M.

In: PLANT CELL TISSUE AND ORGAN CULTURE, Vol. 128, 2017, pag. 85-95.

Risultato della ricerca: Article

@article{a9c4c2f68d794fc9b34da474d8a7344a,
title = "Microspore embryogenesis induced through in vitro anther culture of almond (Prunus dulcis Mill.)",
abstract = "Anther culture is one of the most widely used methods to induce gametic embryogenesis. The aim of this investigation was to induce microspore embryogenesis in almond (Prunus dulcis Mill.), through this technique. Anthers were cultured at the vacuolated developmental stage, and seven cultivars, two culture media and two temperature treatments were assessed. Although evidence of the microspore induction was observed in all the genotypes and treatments tested (symmetrical nucleus division and multinucleated structures), calli were produced merely by anthers cultured in the medium P and the regeneration of embryos was detected only in anthers of the cultivars Filippo Ceo, Lauranne and Genco, placed on medium P and subjected to the Control treatment (direct culture at 25 ± 1 °C, without the hot thermal shock at 35 ± 1 °C for 7 days). Characterization by SSR marker analysis of the embryo genotypes revealed that the regenerants had a single allele for each locus whereas the parent cultivar was heterozygous, indicating their development from haploid microspores. This study reports the evidence of gametic embryogenesis and, particularly, of microspore embryogenesis through in vitro anther culture, in almond, and, for the first time to our knowledge, the production of homozygous embryos.",
author = "{Cim{\`o}, G.; German{\`a}, M.} and Maria Germana' and Annalisa Marchese and Giuseppe Cimo'",
year = "2017",
language = "English",
volume = "128",
pages = "85--95",
journal = "Plant Cell, Tissue and Organ Culture",
issn = "0167-6857",
publisher = "Springer Netherlands",

}

TY - JOUR

T1 - Microspore embryogenesis induced through in vitro anther culture of almond (Prunus dulcis Mill.)

AU - Cimò, G.; Germanà, M.

AU - Germana', Maria

AU - Marchese, Annalisa

AU - Cimo', Giuseppe

PY - 2017

Y1 - 2017

N2 - Anther culture is one of the most widely used methods to induce gametic embryogenesis. The aim of this investigation was to induce microspore embryogenesis in almond (Prunus dulcis Mill.), through this technique. Anthers were cultured at the vacuolated developmental stage, and seven cultivars, two culture media and two temperature treatments were assessed. Although evidence of the microspore induction was observed in all the genotypes and treatments tested (symmetrical nucleus division and multinucleated structures), calli were produced merely by anthers cultured in the medium P and the regeneration of embryos was detected only in anthers of the cultivars Filippo Ceo, Lauranne and Genco, placed on medium P and subjected to the Control treatment (direct culture at 25 ± 1 °C, without the hot thermal shock at 35 ± 1 °C for 7 days). Characterization by SSR marker analysis of the embryo genotypes revealed that the regenerants had a single allele for each locus whereas the parent cultivar was heterozygous, indicating their development from haploid microspores. This study reports the evidence of gametic embryogenesis and, particularly, of microspore embryogenesis through in vitro anther culture, in almond, and, for the first time to our knowledge, the production of homozygous embryos.

AB - Anther culture is one of the most widely used methods to induce gametic embryogenesis. The aim of this investigation was to induce microspore embryogenesis in almond (Prunus dulcis Mill.), through this technique. Anthers were cultured at the vacuolated developmental stage, and seven cultivars, two culture media and two temperature treatments were assessed. Although evidence of the microspore induction was observed in all the genotypes and treatments tested (symmetrical nucleus division and multinucleated structures), calli were produced merely by anthers cultured in the medium P and the regeneration of embryos was detected only in anthers of the cultivars Filippo Ceo, Lauranne and Genco, placed on medium P and subjected to the Control treatment (direct culture at 25 ± 1 °C, without the hot thermal shock at 35 ± 1 °C for 7 days). Characterization by SSR marker analysis of the embryo genotypes revealed that the regenerants had a single allele for each locus whereas the parent cultivar was heterozygous, indicating their development from haploid microspores. This study reports the evidence of gametic embryogenesis and, particularly, of microspore embryogenesis through in vitro anther culture, in almond, and, for the first time to our knowledge, the production of homozygous embryos.

UR - http://hdl.handle.net/10447/219470

M3 - Article

VL - 128

SP - 85

EP - 95

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

SN - 0167-6857

ER -