Ageratum houstonianum is a bedding and flowering potted plant originated fromCentral America which is generally propagate by seed. In this report a preliminary invitro technique for propagation of A. houstonianum was investigated. In vitrogerminated seeds were used to establish aseptic shoot cultures of several clones.Seedling stem segments bearing 3-4 nodes were placed on Murashige and Skoog (MS)basal medium plus 20 g L-1 sucrose, 8.0 g L-1 Agar to induce axillary shootdevelopment. Axillary shoots were subcultured into the same medium and nodalsegments were sectioned and subcultured to increase the stock of shoot cultures.Shoot cultures of the selected clone AG14 were used to accomplish in vitropropagation experiments. The MS medium, augmented with different concentrationsof N-6-benzyladenine (BA) (0, 0.88, 1.78, 3.55, or 7.1 μM) either singly or incombination with indole-3-acetic acid (IAA) at 5.7 μM, as potential medium for shootmultiplication by single node segments was tested. The highest rate of axillary shootproliferation was induced on the medium supplemented with 7.1 μM BA. Explantswere divided, subcultured and continued to proliferate shoots. A proliferation rate of5.2 shoots per single node explants every four weeks occurred. The highest number ofnode per axillary shoots after 8 week culture (4.5 nodes) was obtained in the mediumwith 0.88 μM BA and 5.7 μM IAA. The elongated axillary shoots could be in turnsectioned and subcultured to form an elongated stem again. Five IAA concentrations(0, 1.14, 2.28, 4.57, 5.71 μM) were tested to determine the optimum conditions for invitro rooting of microshoots. The highest number of roots per microshoots wasobtained with IAA at 2.28 μM. Ninety percent of the in vitro rooted plantlets weresuccessfully established in soil. This micropropagation system of A. houstonianumbased on axillary shoot development from nodal segments, followed by in vitrorooting, might be used for rapid and efficient mass propagation of improvedselections and disease-free germplasm.
|Numero di pagine||9|
|Stato di pubblicazione||Published - 2017|
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