Abstract
| Lingua originale | English |
|---|---|
| pagine (da-a) | 97-104 |
| Numero di pagine | 8 |
| Rivista | Journal of Enzyme Inhibition and Medicinal Chemistry |
| Volume | 25 |
| Stato di pubblicazione | Published - 2010 |
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All Science Journal Classification (ASJC) codes
- Pharmacology
- Drug Discovery
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Metabolite profile and in vitro activities of Phagnalon saxatile (L.) Cass. relevant to treatment of Alzheimer's disease. / Bruno, Maurizio; Rigano, Daniela; Menichini, Francesco; Conforti, Filomena; Senatore, Felice; Bruno, Maurizio; Loizzo, Monica Rosa; Formisano, Carmen.
In: Journal of Enzyme Inhibition and Medicinal Chemistry, Vol. 25, 2010, pag. 97-104.Risultato della ricerca: Article
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TY - JOUR
T1 - Metabolite profile and in vitro activities of Phagnalon saxatile (L.) Cass. relevant to treatment of Alzheimer's disease
AU - Bruno, Maurizio
AU - Rigano, Daniela
AU - Menichini, Francesco
AU - Conforti, Filomena
AU - Senatore, Felice
AU - Bruno, Maurizio
AU - Loizzo, Monica Rosa
AU - Formisano, Carmen
PY - 2010
Y1 - 2010
N2 - The present study describes for the first time the in vitro properties (inhibition of NO production and anticholinesterase) of Phagnalon saxatile (L.) Cass. (Asteraceae). The methanolic extract showed antioxidant activity that was measured by DPPH assay and β-carotene bleaching test. The same extract inhibited NO production in the murine monocytic macrophage cell line RAW 264.7. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition was assessed by modifications of Ellman’s method. Purification of the MeOH extract of P. saxatile allowed the isolation of phenolic compounds. Among them, the compounds that most effectively inhibited lipopolysaccharide-induced NO production were caffeic acid and methylchlorogenic acid, with IC50 values of 7μg/mL and 12μg/mL, respectively. Luteolin and 3,5-dicaffeoylquinic acid exhibited the most promising activity against AChE with an IC50 of 25.2 and 54.5μg/mL, respectively, while caffeic acid and luteolin exhibited higher activity against BChE with an IC50 of 32.2 and 37.2μg/mL, respectively.
AB - The present study describes for the first time the in vitro properties (inhibition of NO production and anticholinesterase) of Phagnalon saxatile (L.) Cass. (Asteraceae). The methanolic extract showed antioxidant activity that was measured by DPPH assay and β-carotene bleaching test. The same extract inhibited NO production in the murine monocytic macrophage cell line RAW 264.7. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition was assessed by modifications of Ellman’s method. Purification of the MeOH extract of P. saxatile allowed the isolation of phenolic compounds. Among them, the compounds that most effectively inhibited lipopolysaccharide-induced NO production were caffeic acid and methylchlorogenic acid, with IC50 values of 7μg/mL and 12μg/mL, respectively. Luteolin and 3,5-dicaffeoylquinic acid exhibited the most promising activity against AChE with an IC50 of 25.2 and 54.5μg/mL, respectively, while caffeic acid and luteolin exhibited higher activity against BChE with an IC50 of 32.2 and 37.2μg/mL, respectively.
KW - Phagnalon saxatile; Asteraceae; phenolic compounds; inhibition of NO production; Alzheimer's disease
UR - http://hdl.handle.net/10447/54914
M3 - Article
VL - 25
SP - 97
EP - 104
JO - Journal of Enzyme Inhibition and Medicinal Chemistry
JF - Journal of Enzyme Inhibition and Medicinal Chemistry
SN - 1475-6366
ER -