Metabolite profile and in vitro activities of Phagnalon saxatile (L.) Cass. relevant to treatment of Alzheimer's disease

Maurizio Bruno, Daniela Rigano, Francesco Menichini, Filomena Conforti, Felice Senatore, Maurizio Bruno, Monica Rosa Loizzo, Carmen Formisano

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Abstract

The present study describes for the first time the in vitro properties (inhibition of NO production and anticholinesterase) of Phagnalon saxatile (L.) Cass. (Asteraceae). The methanolic extract showed antioxidant activity that was measured by DPPH assay and β-carotene bleaching test. The same extract inhibited NO production in the murine monocytic macrophage cell line RAW 264.7. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition was assessed by modifications of Ellman’s method. Purification of the MeOH extract of P. saxatile allowed the isolation of phenolic compounds. Among them, the compounds that most effectively inhibited lipopolysaccharide-induced NO production were caffeic acid and methylchlorogenic acid, with IC50 values of 7μg/mL and 12μg/mL, respectively. Luteolin and 3,5-dicaffeoylquinic acid exhibited the most promising activity against AChE with an IC50 of 25.2 and 54.5μg/mL, respectively, while caffeic acid and luteolin exhibited higher activity against BChE with an IC50 of 32.2 and 37.2μg/mL, respectively.
Lingua originaleEnglish
pagine (da-a)97-104
Numero di pagine8
RivistaJournal of Enzyme Inhibition and Medicinal Chemistry
Volume25
Stato di pubblicazionePublished - 2010

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All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cita questo

Bruno, M., Rigano, D., Menichini, F., Conforti, F., Senatore, F., Bruno, M., Loizzo, M. R., & Formisano, C. (2010). Metabolite profile and in vitro activities of Phagnalon saxatile (L.) Cass. relevant to treatment of Alzheimer's disease. Journal of Enzyme Inhibition and Medicinal Chemistry, 25, 97-104.