Metabolite profile and in vitro activities of Phagnalon saxatile (L.) Cass. relevant to treatment of Alzheimer's didease

Maurizio Bruno, Daniela Rigano, Francesco Menichini, Filomena Conforti, Felice Senatore, Monica Rosa Loizzo, Carmen Formisano

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23 Citazioni (Scopus)

Abstract

The present study describes for the first time the in vitro properties (inhibition of NO production and anticholinesterase)of Phagnalon saxatile (L.) Cass. (Asteraceae). The methanolic extract showed antioxidant activitythat was measured by DPPH assay and β-carotene bleaching test. The same extract inhibited NO production inthe murine monocytic macrophage cell line RAW 264.7. Acetylcholinesterase (AChE) and butyrylcholinesterase(BChE) inhibition was assessed by modifications of Ellman’s method. Purification of the MeOH extract of P. saxatileallowed the isolation of phenolic compounds. Among them, the compounds that most effectively inhibitedlipopolysaccharide-induced NO production were caffeic acid and methylchlorogenic acid, with IC50 values of7μg/mL and 12μg/mL, respectively. Luteolin and 3,5-dicaffeoylquinic acid exhibited the most promising activityagainst AChE with an IC50 of 25.2 and 54.5μg/mL, respectively, while caffeic acid and luteolin exhibited higheractivity against BChE with an IC50 of 32.2 and 37.2μg/mL, respectively.
Lingua originaleEnglish
pagine (da-a)97-104
Numero di pagine8
RivistaJournal of Enzyme Inhibition and Medicinal Chemistry
Volume25
Stato di pubblicazionePublished - 2009

All Science Journal Classification (ASJC) codes

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