Mechanisms underlying the inhibitory effects induced by pituitary adenylate cyclase-activating peptide in mouse ileum

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Abstract

The aim of this study was to investigate the signal transduction mechanisms underlying the inhibitory effect induced by pituitary adenylatecyclase activating peptide (PACAP-27) on the spontaneous contractile activity of longitudinal muscle of mouse ileum. Mechanical activity of ilealsegments was recorded isometrically in vitro. PACAP-27 produced apamin-sensitive reduction of the amplitude of the spontaneous contractions.9-(Tetrahydro-2-furanyl)-9H-purin-6-amine (SQ 22,536), adenylate cyclase inhibitor, or genistein and tyrphostin 25, tyrosine kinase inhibitors,had negligible effects on PACAP-27-induced inhibition. PACAP-27 effects were significantly inhibited by U-73122, phopholipase C (PLC)inhibitor, by 2-aminoethoxy-diphenylborate (2-APB), permeable blocker of inositol 1,4,5-triphosphate (IP3) receptors and by depletion of Ca2+stores with cyclopiazonic acid or thapsigargin. Ryanodine did not reduce PACAP-27-inhibitory responses.We suggest that, in mouse ileum, the inhibitory responses to PACAP-27 involve stimulation of PLC, increased production of IP3 and localisedCa2+ release from intracellular stores, which could provide the opening of apamin-sensitive Ca2+-dependent K+ channels.
Lingua originaleEnglish
pagine (da-a)133-138
Numero di pagine6
RivistaEuropean Journal of Pharmacology
Volume521
Stato di pubblicazionePublished - 2005

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Pituitary Adenylate Cyclase-Activating Polypeptide
Ileum
Adenylyl Cyclases
Peptides
Apamin
Inositol 1,4,5-Trisphosphate Receptors
Ryanodine
Inositol 1,4,5-Trisphosphate
Thapsigargin
Genistein
Protein-Tyrosine Kinases
Amines
Signal Transduction
Muscles

All Science Journal Classification (ASJC) codes

  • Pharmacology

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title = "Mechanisms underlying the inhibitory effects induced by pituitary adenylate cyclase-activating peptide in mouse ileum",
abstract = "The aim of this study was to investigate the signal transduction mechanisms underlying the inhibitory effect induced by pituitary adenylatecyclase activating peptide (PACAP-27) on the spontaneous contractile activity of longitudinal muscle of mouse ileum. Mechanical activity of ilealsegments was recorded isometrically in vitro. PACAP-27 produced apamin-sensitive reduction of the amplitude of the spontaneous contractions.9-(Tetrahydro-2-furanyl)-9H-purin-6-amine (SQ 22,536), adenylate cyclase inhibitor, or genistein and tyrphostin 25, tyrosine kinase inhibitors,had negligible effects on PACAP-27-induced inhibition. PACAP-27 effects were significantly inhibited by U-73122, phopholipase C (PLC)inhibitor, by 2-aminoethoxy-diphenylborate (2-APB), permeable blocker of inositol 1,4,5-triphosphate (IP3) receptors and by depletion of Ca2+stores with cyclopiazonic acid or thapsigargin. Ryanodine did not reduce PACAP-27-inhibitory responses.We suggest that, in mouse ileum, the inhibitory responses to PACAP-27 involve stimulation of PLC, increased production of IP3 and localisedCa2+ release from intracellular stores, which could provide the opening of apamin-sensitive Ca2+-dependent K+ channels.",
author = "Serio, {Rosa Maria} and Zizzo, {Maria Grazia} and Flavia Mule' and Zizzo, {Maria Grazia}",
year = "2005",
language = "English",
volume = "521",
pages = "133--138",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",

}

TY - JOUR

T1 - Mechanisms underlying the inhibitory effects induced by pituitary adenylate cyclase-activating peptide in mouse ileum

AU - Serio, Rosa Maria

AU - Zizzo, Maria Grazia

AU - Mule', Flavia

AU - Zizzo, Maria Grazia

PY - 2005

Y1 - 2005

N2 - The aim of this study was to investigate the signal transduction mechanisms underlying the inhibitory effect induced by pituitary adenylatecyclase activating peptide (PACAP-27) on the spontaneous contractile activity of longitudinal muscle of mouse ileum. Mechanical activity of ilealsegments was recorded isometrically in vitro. PACAP-27 produced apamin-sensitive reduction of the amplitude of the spontaneous contractions.9-(Tetrahydro-2-furanyl)-9H-purin-6-amine (SQ 22,536), adenylate cyclase inhibitor, or genistein and tyrphostin 25, tyrosine kinase inhibitors,had negligible effects on PACAP-27-induced inhibition. PACAP-27 effects were significantly inhibited by U-73122, phopholipase C (PLC)inhibitor, by 2-aminoethoxy-diphenylborate (2-APB), permeable blocker of inositol 1,4,5-triphosphate (IP3) receptors and by depletion of Ca2+stores with cyclopiazonic acid or thapsigargin. Ryanodine did not reduce PACAP-27-inhibitory responses.We suggest that, in mouse ileum, the inhibitory responses to PACAP-27 involve stimulation of PLC, increased production of IP3 and localisedCa2+ release from intracellular stores, which could provide the opening of apamin-sensitive Ca2+-dependent K+ channels.

AB - The aim of this study was to investigate the signal transduction mechanisms underlying the inhibitory effect induced by pituitary adenylatecyclase activating peptide (PACAP-27) on the spontaneous contractile activity of longitudinal muscle of mouse ileum. Mechanical activity of ilealsegments was recorded isometrically in vitro. PACAP-27 produced apamin-sensitive reduction of the amplitude of the spontaneous contractions.9-(Tetrahydro-2-furanyl)-9H-purin-6-amine (SQ 22,536), adenylate cyclase inhibitor, or genistein and tyrphostin 25, tyrosine kinase inhibitors,had negligible effects on PACAP-27-induced inhibition. PACAP-27 effects were significantly inhibited by U-73122, phopholipase C (PLC)inhibitor, by 2-aminoethoxy-diphenylborate (2-APB), permeable blocker of inositol 1,4,5-triphosphate (IP3) receptors and by depletion of Ca2+stores with cyclopiazonic acid or thapsigargin. Ryanodine did not reduce PACAP-27-inhibitory responses.We suggest that, in mouse ileum, the inhibitory responses to PACAP-27 involve stimulation of PLC, increased production of IP3 and localisedCa2+ release from intracellular stores, which could provide the opening of apamin-sensitive Ca2+-dependent K+ channels.

UR - http://hdl.handle.net/10447/2186

M3 - Article

VL - 521

SP - 133

EP - 138

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

ER -