Abstract
| Lingua originale | English |
|---|---|
| pagine (da-a) | 133-138 |
| Numero di pagine | 6 |
| Rivista | European Journal of Pharmacology |
| Volume | 521 |
| Stato di pubblicazione | Published - 2005 |
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All Science Journal Classification (ASJC) codes
- Pharmacology
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Mechanisms underlying the inhibitory effects induced by pituitary adenylate cyclase-activating peptide in mouse ileum. / Serio, Rosa Maria; Zizzo, Maria Grazia; Mule', Flavia; Zizzo, Maria Grazia.
In: European Journal of Pharmacology, Vol. 521, 2005, pag. 133-138.Risultato della ricerca: Article
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TY - JOUR
T1 - Mechanisms underlying the inhibitory effects induced by pituitary adenylate cyclase-activating peptide in mouse ileum
AU - Serio, Rosa Maria
AU - Zizzo, Maria Grazia
AU - Mule', Flavia
AU - Zizzo, Maria Grazia
PY - 2005
Y1 - 2005
N2 - The aim of this study was to investigate the signal transduction mechanisms underlying the inhibitory effect induced by pituitary adenylatecyclase activating peptide (PACAP-27) on the spontaneous contractile activity of longitudinal muscle of mouse ileum. Mechanical activity of ilealsegments was recorded isometrically in vitro. PACAP-27 produced apamin-sensitive reduction of the amplitude of the spontaneous contractions.9-(Tetrahydro-2-furanyl)-9H-purin-6-amine (SQ 22,536), adenylate cyclase inhibitor, or genistein and tyrphostin 25, tyrosine kinase inhibitors,had negligible effects on PACAP-27-induced inhibition. PACAP-27 effects were significantly inhibited by U-73122, phopholipase C (PLC)inhibitor, by 2-aminoethoxy-diphenylborate (2-APB), permeable blocker of inositol 1,4,5-triphosphate (IP3) receptors and by depletion of Ca2+stores with cyclopiazonic acid or thapsigargin. Ryanodine did not reduce PACAP-27-inhibitory responses.We suggest that, in mouse ileum, the inhibitory responses to PACAP-27 involve stimulation of PLC, increased production of IP3 and localisedCa2+ release from intracellular stores, which could provide the opening of apamin-sensitive Ca2+-dependent K+ channels.
AB - The aim of this study was to investigate the signal transduction mechanisms underlying the inhibitory effect induced by pituitary adenylatecyclase activating peptide (PACAP-27) on the spontaneous contractile activity of longitudinal muscle of mouse ileum. Mechanical activity of ilealsegments was recorded isometrically in vitro. PACAP-27 produced apamin-sensitive reduction of the amplitude of the spontaneous contractions.9-(Tetrahydro-2-furanyl)-9H-purin-6-amine (SQ 22,536), adenylate cyclase inhibitor, or genistein and tyrphostin 25, tyrosine kinase inhibitors,had negligible effects on PACAP-27-induced inhibition. PACAP-27 effects were significantly inhibited by U-73122, phopholipase C (PLC)inhibitor, by 2-aminoethoxy-diphenylborate (2-APB), permeable blocker of inositol 1,4,5-triphosphate (IP3) receptors and by depletion of Ca2+stores with cyclopiazonic acid or thapsigargin. Ryanodine did not reduce PACAP-27-inhibitory responses.We suggest that, in mouse ileum, the inhibitory responses to PACAP-27 involve stimulation of PLC, increased production of IP3 and localisedCa2+ release from intracellular stores, which could provide the opening of apamin-sensitive Ca2+-dependent K+ channels.
UR - http://hdl.handle.net/10447/2186
M3 - Article
VL - 521
SP - 133
EP - 138
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
ER -