Macrophage-mediated clearance of cells undergoing caspase-3-independent death

Michela Giuliano, Cohen, Devitt, Macfarlane, Chris D. Gregory, Parker, Turner

Risultato della ricerca: Article

10 Citazioni (Scopus)

Abstract

Little is known of the functions of caspases in mediating the surface changes required for phagocytosis of dying cells. Here we investigate the role played by the effector caspase, caspase-3 in this process using the caspase-3-defective MCF-7 breast carcinoma line and derived caspase-3-expressing transfectants. Our results indicate that, while certain typical features of apoptosis induced by etoposide - namely classical morphological changes and the ability to degrade DNA into oligonucleosomal fragments - are caspase-3-dependent, loss of cell adhesion to plastic and the capacity to interact with, and to be phagocytosed by, human monocyte-derived macrophages - both by CD14-dependent and CD14-independent mechanisms - do not require caspase-3. Furthermore, both etoposide-induced caspase-3-positive and -negative MCF-7 cells suppressed proinflammatory cytokine release by macrophages. These results demonstrate directly that cell surface changes that are sufficient for anti-inflammatory clearance by human macrophages can be regulated independently of stereotypical features of the apoptosis programme that require caspase-3.
Lingua originaleEnglish
pagine (da-a)302-312
Numero di pagine11
RivistaCell Death and Differentiation
Volume10
Stato di pubblicazionePublished - 2003

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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