LPS injection reprograms the expression and the 3'UTR of a CAP gene by alternative polyadenylation and the formation of a GAIT element in Ciona intestinalis

Daniela Parrinello, Matteo Cammarata, Aiti Vizzini, Paolo Colombo, Matteo Cammarata, Maria Antonietta Sanfratello, Valeria Longo, Daniela Parrinello, Angela Bonura, Aiti Vizzini

Risultato della ricerca: Article

5 Citazioni (Scopus)

Abstract

tThe diversification of cellular functions is one of the major characteristics of multicellular organ-isms which allow cells to modulate their gene expression, leading to the formation of transcripts andproteins with different functions and concentrations in response to different stimuli. CAP genes rep-resent a widespread family of proteins belonging to the cysteine-rich secretory protein, antigen 5 andpathogenesis-related 1 superfamily which, it has been proposed, play key roles in the infection processand the modulation of immune responses in host animals. The ascidian Ciona intestinalis represents agroup of proto-chordates with an exclusively innate immune system that has been widely studied inthe field of comparative and developmental immunology. Using this biological system, we describe theidentification of a novel APA mechanism by which an intronic polyadenylation signal is activated by LPSinjection, leading to the formation of a shorter CAP mRNA capable of expressing the first CAP exon plus19 amino acid residues whose sequence is contained within the first intron of the annotated gene. Fur-thermore, such an APA event causes the expression of a translational controlling cis-acting GAIT elementwhich is not present in the previously isolated CAP isoform and identified in the 3-UTR of other immune-related genes, suggesting an intriguing scenario in which both transcriptional and post-transcriptionalcontrol mechanisms are involved in the activation of the CAP gene during inflammatory response in C.intestinalis.
Lingua originaleEnglish
pagine (da-a)174-183
Numero di pagine10
RivistaMolecular Immunology
Volume77
Stato di pubblicazionePublished - 2016

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Ciona intestinalis
Polyadenylation
3' Untranslated Regions
Injections
Chordata
Genes
Urochordata
Allergy and Immunology
Introns
Transcriptional Activation
Cysteine
Exons
Immune System
Protein Isoforms
Proteins
Gene Expression
Antigens
Amino Acids
Messenger RNA
Infection

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

Cita questo

LPS injection reprograms the expression and the 3'UTR of a CAP gene by alternative polyadenylation and the formation of a GAIT element in Ciona intestinalis. / Parrinello, Daniela; Cammarata, Matteo; Vizzini, Aiti; Colombo, Paolo; Cammarata, Matteo; Sanfratello, Maria Antonietta; Longo, Valeria; Parrinello, Daniela; Bonura, Angela; Vizzini, Aiti.

In: Molecular Immunology, Vol. 77, 2016, pag. 174-183.

Risultato della ricerca: Article

Parrinello, Daniela ; Cammarata, Matteo ; Vizzini, Aiti ; Colombo, Paolo ; Cammarata, Matteo ; Sanfratello, Maria Antonietta ; Longo, Valeria ; Parrinello, Daniela ; Bonura, Angela ; Vizzini, Aiti. / LPS injection reprograms the expression and the 3'UTR of a CAP gene by alternative polyadenylation and the formation of a GAIT element in Ciona intestinalis. In: Molecular Immunology. 2016 ; Vol. 77. pagg. 174-183.
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abstract = "tThe diversification of cellular functions is one of the major characteristics of multicellular organ-isms which allow cells to modulate their gene expression, leading to the formation of transcripts andproteins with different functions and concentrations in response to different stimuli. CAP genes rep-resent a widespread family of proteins belonging to the cysteine-rich secretory protein, antigen 5 andpathogenesis-related 1 superfamily which, it has been proposed, play key roles in the infection processand the modulation of immune responses in host animals. The ascidian Ciona intestinalis represents agroup of proto-chordates with an exclusively innate immune system that has been widely studied inthe field of comparative and developmental immunology. Using this biological system, we describe theidentification of a novel APA mechanism by which an intronic polyadenylation signal is activated by LPSinjection, leading to the formation of a shorter CAP mRNA capable of expressing the first CAP exon plus19 amino acid residues whose sequence is contained within the first intron of the annotated gene. Fur-thermore, such an APA event causes the expression of a translational controlling cis-acting GAIT elementwhich is not present in the previously isolated CAP isoform and identified in the 3-UTR of other immune-related genes, suggesting an intriguing scenario in which both transcriptional and post-transcriptionalcontrol mechanisms are involved in the activation of the CAP gene during inflammatory response in C.intestinalis.",
author = "Daniela Parrinello and Matteo Cammarata and Aiti Vizzini and Paolo Colombo and Matteo Cammarata and Sanfratello, {Maria Antonietta} and Valeria Longo and Daniela Parrinello and Angela Bonura and Aiti Vizzini",
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T1 - LPS injection reprograms the expression and the 3'UTR of a CAP gene by alternative polyadenylation and the formation of a GAIT element in Ciona intestinalis

AU - Parrinello, Daniela

AU - Cammarata, Matteo

AU - Vizzini, Aiti

AU - Colombo, Paolo

AU - Cammarata, Matteo

AU - Sanfratello, Maria Antonietta

AU - Longo, Valeria

AU - Parrinello, Daniela

AU - Bonura, Angela

AU - Vizzini, Aiti

PY - 2016

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N2 - tThe diversification of cellular functions is one of the major characteristics of multicellular organ-isms which allow cells to modulate their gene expression, leading to the formation of transcripts andproteins with different functions and concentrations in response to different stimuli. CAP genes rep-resent a widespread family of proteins belonging to the cysteine-rich secretory protein, antigen 5 andpathogenesis-related 1 superfamily which, it has been proposed, play key roles in the infection processand the modulation of immune responses in host animals. The ascidian Ciona intestinalis represents agroup of proto-chordates with an exclusively innate immune system that has been widely studied inthe field of comparative and developmental immunology. Using this biological system, we describe theidentification of a novel APA mechanism by which an intronic polyadenylation signal is activated by LPSinjection, leading to the formation of a shorter CAP mRNA capable of expressing the first CAP exon plus19 amino acid residues whose sequence is contained within the first intron of the annotated gene. Fur-thermore, such an APA event causes the expression of a translational controlling cis-acting GAIT elementwhich is not present in the previously isolated CAP isoform and identified in the 3-UTR of other immune-related genes, suggesting an intriguing scenario in which both transcriptional and post-transcriptionalcontrol mechanisms are involved in the activation of the CAP gene during inflammatory response in C.intestinalis.

AB - tThe diversification of cellular functions is one of the major characteristics of multicellular organ-isms which allow cells to modulate their gene expression, leading to the formation of transcripts andproteins with different functions and concentrations in response to different stimuli. CAP genes rep-resent a widespread family of proteins belonging to the cysteine-rich secretory protein, antigen 5 andpathogenesis-related 1 superfamily which, it has been proposed, play key roles in the infection processand the modulation of immune responses in host animals. The ascidian Ciona intestinalis represents agroup of proto-chordates with an exclusively innate immune system that has been widely studied inthe field of comparative and developmental immunology. Using this biological system, we describe theidentification of a novel APA mechanism by which an intronic polyadenylation signal is activated by LPSinjection, leading to the formation of a shorter CAP mRNA capable of expressing the first CAP exon plus19 amino acid residues whose sequence is contained within the first intron of the annotated gene. Fur-thermore, such an APA event causes the expression of a translational controlling cis-acting GAIT elementwhich is not present in the previously isolated CAP isoform and identified in the 3-UTR of other immune-related genes, suggesting an intriguing scenario in which both transcriptional and post-transcriptionalcontrol mechanisms are involved in the activation of the CAP gene during inflammatory response in C.intestinalis.

UR - http://hdl.handle.net/10447/196553

M3 - Article

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JO - Molecular Immunology

JF - Molecular Immunology

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