LPS Challenge Regulates Gene Expression and Tissue Localization of a Ciona intestinalis Gene through an Alternative Polyadenylation Mechanism

Daniela Parrinello, Aiti Vizzini, Maria Antonietta Sanfratello, Daniela Parrinello, Angela Bonura, Aiti Vizzini, Paolo Colombo, Maria Antonietta Sanfratello, Valeria Longo

Risultato della ricerca: Article

9 Citazioni (Scopus)

Abstract

subtractive hybridization strategy for the identification of differentially expressed genes was performed between LPSchallenged and naive Ciona intestinalis. This strategy allowed the characterization of two transcripts (Ci8short and Ci8long) generated by the use of two Alternative Polyadenylation sites. The Ci8long transcript contains a protein domain with relevant homology to several components of the Receptor Transporting Protein (RTP) family not present in the Ci8short mRNA. By means of Real Time PCR and Northern Blot, the Ci8short and Ci8long transcripts showed a different pattern of gene expression with the Ci8short mRNA being strongly activated after LPS injection in the pharynx. In situ hybridization analysis demonstrated that the activation of the APA site also influenced the tissue localization of the Ci8short transcript. This analysis showed that the Ci8long mRNA was expressed in hemocytes meanwhile the Ci8short mRNA was highly transcribed also in vessel endothelial cells and in the epithelium of pharynx. These findings demonstrated that regulation of gene expression based on different polyadenylation sites is an ancestral powerful strategy influencing both the level of expression and tissue distribution of alternative transcripts.
Lingua originaleEnglish
pagine (da-a)-
Numero di pagine12
RivistaPLoS One
Volume8
Stato di pubblicazionePublished - 2013

Fingerprint

Ciona intestinalis
Polyadenylation
pharynx
Gene expression
Genes
Pharynx
Gene Expression
gene expression
Messenger RNA
suppression subtractive hybridization
tissue distribution
hemocytes
sequence homology
Northern blotting
Tissue
endothelial cells
Hemocytes
quantitative polymerase chain reaction
epithelium
genes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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LPS Challenge Regulates Gene Expression and Tissue Localization of a Ciona intestinalis Gene through an Alternative Polyadenylation Mechanism. / Parrinello, Daniela; Vizzini, Aiti; Sanfratello, Maria Antonietta; Parrinello, Daniela; Bonura, Angela; Vizzini, Aiti; Colombo, Paolo; Sanfratello, Maria Antonietta; Longo, Valeria.

In: PLoS One, Vol. 8, 2013, pag. -.

Risultato della ricerca: Article

Parrinello, Daniela ; Vizzini, Aiti ; Sanfratello, Maria Antonietta ; Parrinello, Daniela ; Bonura, Angela ; Vizzini, Aiti ; Colombo, Paolo ; Sanfratello, Maria Antonietta ; Longo, Valeria. / LPS Challenge Regulates Gene Expression and Tissue Localization of a Ciona intestinalis Gene through an Alternative Polyadenylation Mechanism. In: PLoS One. 2013 ; Vol. 8. pagg. -.
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AU - Parrinello, Daniela

AU - Vizzini, Aiti

AU - Sanfratello, Maria Antonietta

AU - Parrinello, Daniela

AU - Bonura, Angela

AU - Vizzini, Aiti

AU - Colombo, Paolo

AU - Sanfratello, Maria Antonietta

AU - Longo, Valeria

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N2 - subtractive hybridization strategy for the identification of differentially expressed genes was performed between LPSchallenged and naive Ciona intestinalis. This strategy allowed the characterization of two transcripts (Ci8short and Ci8long) generated by the use of two Alternative Polyadenylation sites. The Ci8long transcript contains a protein domain with relevant homology to several components of the Receptor Transporting Protein (RTP) family not present in the Ci8short mRNA. By means of Real Time PCR and Northern Blot, the Ci8short and Ci8long transcripts showed a different pattern of gene expression with the Ci8short mRNA being strongly activated after LPS injection in the pharynx. In situ hybridization analysis demonstrated that the activation of the APA site also influenced the tissue localization of the Ci8short transcript. This analysis showed that the Ci8long mRNA was expressed in hemocytes meanwhile the Ci8short mRNA was highly transcribed also in vessel endothelial cells and in the epithelium of pharynx. These findings demonstrated that regulation of gene expression based on different polyadenylation sites is an ancestral powerful strategy influencing both the level of expression and tissue distribution of alternative transcripts.

AB - subtractive hybridization strategy for the identification of differentially expressed genes was performed between LPSchallenged and naive Ciona intestinalis. This strategy allowed the characterization of two transcripts (Ci8short and Ci8long) generated by the use of two Alternative Polyadenylation sites. The Ci8long transcript contains a protein domain with relevant homology to several components of the Receptor Transporting Protein (RTP) family not present in the Ci8short mRNA. By means of Real Time PCR and Northern Blot, the Ci8short and Ci8long transcripts showed a different pattern of gene expression with the Ci8short mRNA being strongly activated after LPS injection in the pharynx. In situ hybridization analysis demonstrated that the activation of the APA site also influenced the tissue localization of the Ci8short transcript. This analysis showed that the Ci8long mRNA was expressed in hemocytes meanwhile the Ci8short mRNA was highly transcribed also in vessel endothelial cells and in the epithelium of pharynx. These findings demonstrated that regulation of gene expression based on different polyadenylation sites is an ancestral powerful strategy influencing both the level of expression and tissue distribution of alternative transcripts.

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