LPS Challenge Regulates Gene Expression and TissueLocalization of a Ciona intestinalis Gene through anAlternative Polyadenylation Mechanism

Maria Antonietta Sanfratello, Aiti Vizzini, Daniela Parrinello, Paolo Colombo, Maria Antonietta Sanfratello, Valeria Longo, Daniela Parrinello, Angela Bonura, Aiti Vizzini

Risultato della ricerca: Article

9 Citazioni (Scopus)

Abstract

subtractive hybridization strategy for the identification of differentially expressed genes was performed between LPSchallengedand naive Ciona intestinalis. This strategy allowed the characterization of two transcripts (Ci8short and Ci8long)generated by the use of two Alternative Polyadenylation sites. The Ci8long transcript contains a protein domain withrelevant homology to several components of the Receptor Transporting Protein (RTP) family not present in the Ci8shortmRNA. By means of Real Time PCR and Northern Blot, the Ci8short and Ci8long transcripts showed a different pattern ofgene expression with the Ci8short mRNA being strongly activated after LPS injection in the pharynx. In situ hybridizationanalysis demonstrated that the activation of the APA site also influenced the tissue localization of the Ci8short transcript.This analysis showed that the Ci8long mRNA was expressed in hemocytes meanwhile the Ci8short mRNA was highlytranscribed also in vessel endothelial cells and in the epithelium of pharynx. These findings demonstrated that regulation ofgene expression based on different polyadenylation sites is an ancestral powerful strategy influencing both the level ofexpression and tissue distribution of alternative transcripts.
Lingua originaleEnglish
Numero di pagine12
RivistaPLoS One
Volume8
Stato di pubblicazionePublished - 2013

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Ciona intestinalis
Polyadenylation
pharynx
Gene expression
Genes
Pharynx
Gene Expression
gene expression
Messenger RNA
suppression subtractive hybridization
tissue distribution
hemocytes
sequence homology
Northern blotting
Tissue
endothelial cells
Hemocytes
quantitative polymerase chain reaction
epithelium
genes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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LPS Challenge Regulates Gene Expression and TissueLocalization of a Ciona intestinalis Gene through anAlternative Polyadenylation Mechanism. / Sanfratello, Maria Antonietta; Vizzini, Aiti; Parrinello, Daniela; Colombo, Paolo; Sanfratello, Maria Antonietta; Longo, Valeria; Parrinello, Daniela; Bonura, Angela; Vizzini, Aiti.

In: PLoS One, Vol. 8, 2013.

Risultato della ricerca: Article

Sanfratello, Maria Antonietta ; Vizzini, Aiti ; Parrinello, Daniela ; Colombo, Paolo ; Sanfratello, Maria Antonietta ; Longo, Valeria ; Parrinello, Daniela ; Bonura, Angela ; Vizzini, Aiti. / LPS Challenge Regulates Gene Expression and TissueLocalization of a Ciona intestinalis Gene through anAlternative Polyadenylation Mechanism. In: PLoS One. 2013 ; Vol. 8.
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T1 - LPS Challenge Regulates Gene Expression and TissueLocalization of a Ciona intestinalis Gene through anAlternative Polyadenylation Mechanism

AU - Sanfratello, Maria Antonietta

AU - Vizzini, Aiti

AU - Parrinello, Daniela

AU - Colombo, Paolo

AU - Sanfratello, Maria Antonietta

AU - Longo, Valeria

AU - Parrinello, Daniela

AU - Bonura, Angela

AU - Vizzini, Aiti

PY - 2013

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N2 - subtractive hybridization strategy for the identification of differentially expressed genes was performed between LPSchallengedand naive Ciona intestinalis. This strategy allowed the characterization of two transcripts (Ci8short and Ci8long)generated by the use of two Alternative Polyadenylation sites. The Ci8long transcript contains a protein domain withrelevant homology to several components of the Receptor Transporting Protein (RTP) family not present in the Ci8shortmRNA. By means of Real Time PCR and Northern Blot, the Ci8short and Ci8long transcripts showed a different pattern ofgene expression with the Ci8short mRNA being strongly activated after LPS injection in the pharynx. In situ hybridizationanalysis demonstrated that the activation of the APA site also influenced the tissue localization of the Ci8short transcript.This analysis showed that the Ci8long mRNA was expressed in hemocytes meanwhile the Ci8short mRNA was highlytranscribed also in vessel endothelial cells and in the epithelium of pharynx. These findings demonstrated that regulation ofgene expression based on different polyadenylation sites is an ancestral powerful strategy influencing both the level ofexpression and tissue distribution of alternative transcripts.

AB - subtractive hybridization strategy for the identification of differentially expressed genes was performed between LPSchallengedand naive Ciona intestinalis. This strategy allowed the characterization of two transcripts (Ci8short and Ci8long)generated by the use of two Alternative Polyadenylation sites. The Ci8long transcript contains a protein domain withrelevant homology to several components of the Receptor Transporting Protein (RTP) family not present in the Ci8shortmRNA. By means of Real Time PCR and Northern Blot, the Ci8short and Ci8long transcripts showed a different pattern ofgene expression with the Ci8short mRNA being strongly activated after LPS injection in the pharynx. In situ hybridizationanalysis demonstrated that the activation of the APA site also influenced the tissue localization of the Ci8short transcript.This analysis showed that the Ci8long mRNA was expressed in hemocytes meanwhile the Ci8short mRNA was highlytranscribed also in vessel endothelial cells and in the epithelium of pharynx. These findings demonstrated that regulation ofgene expression based on different polyadenylation sites is an ancestral powerful strategy influencing both the level ofexpression and tissue distribution of alternative transcripts.

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