Lower sperm DNA fragmentation after r-FSH administration in functional hypogonadotropic hypogonadism

Maria Carmela Roccheri, Liana Bosco, Anna Maria Brucculeri, Maria Carmela Roccheri, Salvatore Longobardi, Giovanni Ruvolo, Ettore Cittadini, Liana Bosco

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Abstract

AbstractPURPOSE:An observational clinical and molecular study was designed to evaluate the effects of the administration of recombinant human FSH on sperm DNA fragmentation in men with a non-classical form of hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia.METHODS:In the study were included 53 men with a non-classical form of hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia. In all patients, sperm DNA fragmentation index (DFI), assessed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) in situ DNA nick end-labelling (TUNEL) assay, was evaluated before starting the treatment with 150 IU of recombinant human FSH, given three times a week for at least 3 months. Patients' semen analysis and DNA fragmentation index were re-evaluated after the 3-month treatment period.RESULTS:After recombinant human FSH therapy, we did not find any differences in terms of sperm count, motility and morphology. The average DNA fragmentation index was significantly reduced (21.15 vs 15.2, p < 0.05), but we found a significant reduction in patients with high basal DFI values (>15 %), while no significant variation occurred in the patients with DFI values ≤15 %.CONCLUSIONS:Recombinant human FSH administration improves sperm DNA integrity in hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia men with DNA fragmentation index value >15 % .
Lingua originaleEnglish
pagine (da-a)497-503
Numero di pagine7
RivistaJournal of Assisted Reproduction and Genetics
Volume30
Stato di pubblicazionePublished - 2013

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All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Genetics(clinical)

Cita questo

Roccheri, M. C., Bosco, L., Brucculeri, A. M., Roccheri, M. C., Longobardi, S., Ruvolo, G., Cittadini, E., & Bosco, L. (2013). Lower sperm DNA fragmentation after r-FSH administration in functional hypogonadotropic hypogonadism. Journal of Assisted Reproduction and Genetics, 30, 497-503.