Intriguing studies suggest that long non-coding RNAs (lncRNAs) tend to act in cis with neighboringprotein-coding genes. Based on this notion, we assessed whether lncRNAs originate from adjacentgenomic regions of selected protein-coding genes, such as early-H2A histone and strim1, that playimportant functions during development of the sea urchin Paracentrotus lividus.Downregulation of H2A expression relies on the sns5 chromatin insulator placed at the 3'-end of H2A.Northern blot hybridization show that a lncRNA (of ~600 nt), not overlapping with the H2A mRNA, istranscribed from sns5, following H2A expression.The strim1 locus encodes the first echinoderm TRIM-containing factor which functional role has beencharacterized. By RT-PCR and gene-transfer analysis we found that a lncRNA originates from thestrim1 gene promoter, and that its transcription positively correlates with that of strim1.Our preliminary findings are consistent with the notion that lncRNAs might be important regulators fordevelopmental processes.
|Numero di pagine||0|
|Stato di pubblicazione||Published - 2014|