The purification, cloning, sequencing, molecularproperties and expression of a fucose-binding lectin fromthe serum of Dicentrarchus labrax (DlFBL) have beenpreviously reported. We now describe the distribution andexpression of DlFBL during fish ontogeny. Immunohistochemistryand in situ hybridization assays were carried outat various developmental stages (from 10 days posthatchinglarvae to juveniles). Another fucose-bindinglectin, similar to DlFBL in biochemical, immunochemicaland agglutinating properties, was extracted and purifiedfrom eggs and appeared to be localized in the embryo yolksack residual. DlFBL was found in columnar and gobletcells of the intestinal epithelium of larvae (from 20 dayspost-hatching) and juveniles and in parenchymal tissue ofjuveniles. DlFBL mRNA and protein were detected in theintestinal epithelium and in hepatocytes. An amplificationproduct from degenerate primers indicates that lectinisotypes with DlFBL epitopes are expressed in eggs andembryos. Whether the lectin fraction isolated from eggs andembryos includes DlFBL of maternal origin remainsunclear.
|Stato di pubblicazione||Published - 2007|