Localization and Interactions of Melatonin in Dry Cholesterol/Lecithin Mixed Reversed Micelles used as Cell Membrane Models

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Abstract

The state of melatonin confined in dry cholesterol/lecithin mixed reversed micelles dispersed in CCl4 was investigated using H-1-NMR and FT-IR spectroscopies as a function of the melatonin to lecithin molar ratio (R-MLT) and of the cholesterol to lecithin molar ratio (R-CHL). An analysis of experimental results leads to the hypothesis that, independent of R-MLT and as a consequence of anisotropic melatonin/lecithin, melatonin/cholesterol and cholesterol/lecithin interactions, melatonin is totally solubilized in reversed micelles. Melatonin is mainly located in and oriented in the nanodomain constituted by the hydrophilic groups of cholesterol and lecithin. A competition of melatonin and cholesterol for the hydrophilic binding sites of the reversed micelles was observed by changing the R-CHL. Some possible biological implications of the specific interactions governing the solubilization process, the preferential location and the peculiar properties of melatonin confined in cholesterol/lecithin mixed reversed micelles are discussed.
Lingua originaleEnglish
pagine (da-a)292-298
Numero di pagine7
RivistaJournal of Pineal Research
Volume38
Stato di pubblicazionePublished - 2005

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Lecithins
Micelles
Melatonin
Cholesterol
Cell Membrane
Spectrum Analysis
Binding Sites

All Science Journal Classification (ASJC) codes

  • Endocrinology

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@article{0b6af7a2e1b143028ef1a36d4dc132fc,
title = "Localization and Interactions of Melatonin in Dry Cholesterol/Lecithin Mixed Reversed Micelles used as Cell Membrane Models",
abstract = "The state of melatonin confined in dry cholesterol/lecithin mixed reversed micelles dispersed in CCl4 was investigated using H-1-NMR and FT-IR spectroscopies as a function of the melatonin to lecithin molar ratio (R-MLT) and of the cholesterol to lecithin molar ratio (R-CHL). An analysis of experimental results leads to the hypothesis that, independent of R-MLT and as a consequence of anisotropic melatonin/lecithin, melatonin/cholesterol and cholesterol/lecithin interactions, melatonin is totally solubilized in reversed micelles. Melatonin is mainly located in and oriented in the nanodomain constituted by the hydrophilic groups of cholesterol and lecithin. A competition of melatonin and cholesterol for the hydrophilic binding sites of the reversed micelles was observed by changing the R-CHL. Some possible biological implications of the specific interactions governing the solubilization process, the preferential location and the peculiar properties of melatonin confined in cholesterol/lecithin mixed reversed micelles are discussed.",
author = "Leopoldo Ceraulo and Mirella Ferrugia and {Turco Liveri}, Vincenzo and Felice Filizzola and David Bongiorno and Ruggirello, {Angela Monia} and Leopoldo Ceraulo",
year = "2005",
language = "English",
volume = "38",
pages = "292--298",
journal = "Journal of Pineal Research",
issn = "0742-3098",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Localization and Interactions of Melatonin in Dry Cholesterol/Lecithin Mixed Reversed Micelles used as Cell Membrane Models

AU - Ceraulo, Leopoldo

AU - Ferrugia, Mirella

AU - Turco Liveri, Vincenzo

AU - Filizzola, Felice

AU - Bongiorno, David

AU - Ruggirello, Angela Monia

AU - Ceraulo, Leopoldo

PY - 2005

Y1 - 2005

N2 - The state of melatonin confined in dry cholesterol/lecithin mixed reversed micelles dispersed in CCl4 was investigated using H-1-NMR and FT-IR spectroscopies as a function of the melatonin to lecithin molar ratio (R-MLT) and of the cholesterol to lecithin molar ratio (R-CHL). An analysis of experimental results leads to the hypothesis that, independent of R-MLT and as a consequence of anisotropic melatonin/lecithin, melatonin/cholesterol and cholesterol/lecithin interactions, melatonin is totally solubilized in reversed micelles. Melatonin is mainly located in and oriented in the nanodomain constituted by the hydrophilic groups of cholesterol and lecithin. A competition of melatonin and cholesterol for the hydrophilic binding sites of the reversed micelles was observed by changing the R-CHL. Some possible biological implications of the specific interactions governing the solubilization process, the preferential location and the peculiar properties of melatonin confined in cholesterol/lecithin mixed reversed micelles are discussed.

AB - The state of melatonin confined in dry cholesterol/lecithin mixed reversed micelles dispersed in CCl4 was investigated using H-1-NMR and FT-IR spectroscopies as a function of the melatonin to lecithin molar ratio (R-MLT) and of the cholesterol to lecithin molar ratio (R-CHL). An analysis of experimental results leads to the hypothesis that, independent of R-MLT and as a consequence of anisotropic melatonin/lecithin, melatonin/cholesterol and cholesterol/lecithin interactions, melatonin is totally solubilized in reversed micelles. Melatonin is mainly located in and oriented in the nanodomain constituted by the hydrophilic groups of cholesterol and lecithin. A competition of melatonin and cholesterol for the hydrophilic binding sites of the reversed micelles was observed by changing the R-CHL. Some possible biological implications of the specific interactions governing the solubilization process, the preferential location and the peculiar properties of melatonin confined in cholesterol/lecithin mixed reversed micelles are discussed.

UR - http://hdl.handle.net/10447/25068

M3 - Article

VL - 38

SP - 292

EP - 298

JO - Journal of Pineal Research

JF - Journal of Pineal Research

SN - 0742-3098

ER -