Abstract
Parietaria pollen is one of the major causes of allergic reaction in southern Europe, affecting about 30% of all allergic patients in this area. Specific immunotherapy is the only treatment able to modify the natural outcome of the disease by restoring a normal immunity against allergens. The preparation of allergen-solid lipid nanoparticles as delivery vehicles for therapeutic proteins, P. judaica major allergen Par j 2, was investigated. The Par j 2 allergen was expressed in a large amount in Escherichia coli and purified to homogeneity. Its immunological properties were studied by western blotting and enzyme-linked immunosorbent assay inhibition. Solid lipid nanoparticles were obtained by water-in-oil-in-water multiple emulsion method and characterized in terms of mean size and surface charge. These systems (approximately 250 nm diameter and negative surface charge) incorporated recombinant Par j 2 with 40% or greater efficiency. Moreover, the endotoxin level and anaphylactic activity of the empty solid lipid nanoparticles and recombinant Par j 2-loaded solid lipid nanoparticles were evaluated by looking at the overexpression of CD203c marker on human basophils. These results demonstrate that recombinant Par j 2-nanoparticles could be proposed as safe compositions for the development of new therapeutic dosage forms to cure allergic reactions.
Lingua originale | English |
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pagine (da-a) | 2953-2962 |
Numero di pagine | 10 |
Rivista | International Journal of Nanomedicine |
Volume | 6 |
Stato di pubblicazione | Published - 2011 |
All Science Journal Classification (ASJC) codes
- ???subjectarea.asjc.1300.1304???
- ???subjectarea.asjc.1500.1502???
- ???subjectarea.asjc.2500.2502???
- ???subjectarea.asjc.3000.3003???
- ???subjectarea.asjc.3000.3002???
- ???subjectarea.asjc.1600.1605???