Lipid droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging

Matilde Todaro; Simone Di Franco; Giorgio Stassi; Rosanna La Rocca; Luca Tirinato; Roberta Ruffilli; Antonina Benfante; Vijayakumar P. Rajamanickam; Lisette Potze; Mario Malerba; Patrizio Candeloro; Francesco De Angelis; Simone Di Franco; Andrea Falqui; Giorgio Stassi; Jan Paul Medema; Ennio Carbone; Roberto Marotta; Matilde Todaro; Enzo Di Fabrizio; Carlo Liberale

Lipid droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging

Matilde Todaro, Simone Di Franco, Giorgio Stassi, Rosanna La Rocca, Luca Tirinato, Roberta Ruffilli, Antonina Benfante, Vijayakumar P. Rajamanickam, Lisette Potze, Mario Malerba, Patrizio Candeloro, Francesco De Angelis, Simone Di Franco, Andrea Falqui, Giorgio Stassi, Jan Paul Medema, Ennio Carbone, Roberto Marotta, Matilde Todaro, Enzo Di FabrizioCarlo Liberale

Discipline Chirurgiche, Oncologiche e Stomatologiche
Promozione della Salute, Materno-Infantile, di Medicina Interna e Specialistica di Eccellenza “G. D’Alessandro”

Risultato della ricerca: Article › peer review

157 Citazioni (Scopus)

Abstract

The Cancer Stem Cell (CSC) model is describing tumors as a hierarchical organizedsystem and CSCs are suggested to be responsible for cancer recurrenceafter therapy. The identification of specific markers of CSCs is thereforeof paramount importance. Here we show that high levels of Lipid Droplets(LDs) are a distinctive mark of CSCs in colorectal (CR) cancer. This increasedlipid content was clearly revealed by label free Raman spectroscopyand it directly correlates with well‐accepted CR‐CSCs markers as CD133 andWnt pathway activity. By xenotransplantation experiments, we have finallydemonstrated that CR‐CSCs overexpressing LDs retain most tumorigenic potential.A relevant conceptual advance in this work is the demonstration that a cellularorganelle, the Lipid Droplet, is a signature of CSCs, in addition to molecularmarkers.A further functional characterization of LDs could lead soon to design newtarget therapies against CR‐CSCs.

Lingua originale	English
pagine (da-a)	35-44
Numero di pagine	10
Rivista	Stem Cells
Volume	33
Stato di pubblicazione	Published - 2015

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Todaro, M., Di Franco, S., Stassi, G., La Rocca, R., Tirinato, L., Ruffilli, R., Benfante, A., Rajamanickam, V. P., Potze, L., Malerba, M., Candeloro, P., De Angelis, F., Di Franco, S., Falqui, A., Stassi, G., Medema, J. P., Carbone, E., Marotta, R., Todaro, M., ... Liberale, C. (2015). Lipid droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging. Stem Cells, 33, 35-44.

Todaro, M , Di Franco, S , Stassi, G, La Rocca, R, Tirinato, L, Ruffilli, R, Benfante, A, Rajamanickam, VP, Potze, L, Malerba, M, Candeloro, P, De Angelis, F, Di Franco, S, Falqui, A, Stassi, G, Medema, JP, Carbone, E, Marotta, R, Todaro, M, Di Fabrizio, E & Liberale, C 2015, 'Lipid droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging', Stem Cells, vol. 33, pagg. 35-44.

@article{fedeec09c2fc428ab33d4a1a05d3e368,

title = "Lipid droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging",

abstract = "The Cancer Stem Cell (CSC) model is describing tumors as a hierarchical organizedsystem and CSCs are suggested to be responsible for cancer recurrenceafter therapy. The identification of specific markers of CSCs is thereforeof paramount importance. Here we show that high levels of Lipid Droplets(LDs) are a distinctive mark of CSCs in colorectal (CR) cancer. This increasedlipid content was clearly revealed by label free Raman spectroscopyand it directly correlates with well‐accepted CR‐CSCs markers as CD133 andWnt pathway activity. By xenotransplantation experiments, we have finallydemonstrated that CR‐CSCs overexpressing LDs retain most tumorigenic potential.A relevant conceptual advance in this work is the demonstration that a cellularorganelle, the Lipid Droplet, is a signature of CSCs, in addition to molecularmarkers.A further functional characterization of LDs could lead soon to design newtarget therapies against CR‐CSCs.",

author = "Matilde Todaro and {Di Franco}, Simone and Giorgio Stassi and {La Rocca}, Rosanna and Luca Tirinato and Roberta Ruffilli and Antonina Benfante and Rajamanickam, {Vijayakumar P.} and Lisette Potze and Mario Malerba and Patrizio Candeloro and {De Angelis}, Francesco and {Di Franco}, Simone and Andrea Falqui and Giorgio Stassi and Medema, {Jan Paul} and Ennio Carbone and Roberto Marotta and Matilde Todaro and {Di Fabrizio}, Enzo and Carlo Liberale",

year = "2015",

language = "English",

volume = "33",

pages = "35--44",

journal = "Stem Cells",

issn = "1066-5099",

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TY - JOUR

T1 - Lipid droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging

AU - Todaro, Matilde

AU - Di Franco, Simone

AU - Stassi, Giorgio

AU - La Rocca, Rosanna

AU - Tirinato, Luca

AU - Ruffilli, Roberta

AU - Benfante, Antonina

AU - Rajamanickam, Vijayakumar P.

AU - Potze, Lisette

AU - Malerba, Mario

AU - Candeloro, Patrizio

AU - De Angelis, Francesco

AU - Di Franco, Simone

AU - Falqui, Andrea

AU - Stassi, Giorgio

AU - Medema, Jan Paul

AU - Carbone, Ennio

AU - Marotta, Roberto

AU - Todaro, Matilde

AU - Di Fabrizio, Enzo

AU - Liberale, Carlo

PY - 2015

Y1 - 2015

N2 - The Cancer Stem Cell (CSC) model is describing tumors as a hierarchical organizedsystem and CSCs are suggested to be responsible for cancer recurrenceafter therapy. The identification of specific markers of CSCs is thereforeof paramount importance. Here we show that high levels of Lipid Droplets(LDs) are a distinctive mark of CSCs in colorectal (CR) cancer. This increasedlipid content was clearly revealed by label free Raman spectroscopyand it directly correlates with well‐accepted CR‐CSCs markers as CD133 andWnt pathway activity. By xenotransplantation experiments, we have finallydemonstrated that CR‐CSCs overexpressing LDs retain most tumorigenic potential.A relevant conceptual advance in this work is the demonstration that a cellularorganelle, the Lipid Droplet, is a signature of CSCs, in addition to molecularmarkers.A further functional characterization of LDs could lead soon to design newtarget therapies against CR‐CSCs.

AB - The Cancer Stem Cell (CSC) model is describing tumors as a hierarchical organizedsystem and CSCs are suggested to be responsible for cancer recurrenceafter therapy. The identification of specific markers of CSCs is thereforeof paramount importance. Here we show that high levels of Lipid Droplets(LDs) are a distinctive mark of CSCs in colorectal (CR) cancer. This increasedlipid content was clearly revealed by label free Raman spectroscopyand it directly correlates with well‐accepted CR‐CSCs markers as CD133 andWnt pathway activity. By xenotransplantation experiments, we have finallydemonstrated that CR‐CSCs overexpressing LDs retain most tumorigenic potential.A relevant conceptual advance in this work is the demonstration that a cellularorganelle, the Lipid Droplet, is a signature of CSCs, in addition to molecularmarkers.A further functional characterization of LDs could lead soon to design newtarget therapies against CR‐CSCs.

UR - http://hdl.handle.net/10447/122257

M3 - Article

SN - 1066-5099

VL - 33

SP - 35

EP - 44

JO - Stem Cells

JF - Stem Cells

ER -

Lipid droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging

Abstract

All Science Journal Classification (ASJC) codes

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