KINETICS OF INSULIN AGGREGATION: DISENTANGLEMENT OF AMYLOID FIBRILLATION FROM LARGE-SIZE CLUSTER FORMATION

Emanuela Fabiola Craparo, Mauro Manno, Pier Luigi San Biagio, Vincenzo Martorana, Donatella Bulone, Emanuela Fabiola Craparo, Pier Luigi San Biagio

Risultato della ricerca: Articlepeer review

54 Citazioni (Scopus)

Abstract

Kinetics of human insulin aggregation has been studied at pH 1.6 and 60 degrees C, when amyloid fibrils are formed. We developed a novel approach based on the analysis of scattered light intensity distribution, which allows distinguishing between small and large size aggregates. By this method, we observed an exponential growth of fibrillar aggregates implying a heterogeneous aggregation mechanism. Also, the apparent lag time observed, correlated with the major increase of thioflavin T fluorescence, has been assigned to the onset of large size cluster formation.
Lingua originaleEnglish
pagine (da-a)4585-4591
Numero di pagine7
RivistaBiophysical Journal
Volume90
Stato di pubblicazionePublished - 2006

All Science Journal Classification (ASJC) codes

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