2 Citazioni (Scopus)

Abstract

Kefiran is an exopolysaccharide produced by microorganisms present in kefir grains, with several health promoting properties. A optimized protocol was developed for the separation of kefiran from kefir grains, allowing to reach a yield 4÷5 % without using toxic or expensive chemicals. The capability of kefiran to produce scaffold via Thermally Induced Phase Separation (TIPS) technique was investigated and porous scaffolds structure was obtained. Separated kefiran and scaffolds were analyzed via DSC and different thermal properties between purified kefiran and scaffold were revealed. XRD analysis revealed different structure between kefiran and scaffolds. The porous scaffold structure can be modulated by modifiying the thermal path of the solution during the phase separation. The citotoxicity of kefiran was evaluated with cell viability test and a significant faster cell proliferation in the treated colture medium with kefiran was evidenced.
Lingua originaleEnglish
pagine (da-a)181-186
Numero di pagine6
RivistaCHEMICAL ENGINEERING TRANSACTIONS
Volume64
Stato di pubblicazionePublished - 2018

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Scaffolds
Phase separation
Cell proliferation
Microorganisms
kefir grain polysaccharide
Poisons
Thermodynamic properties
Cells
Health

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)

Cita questo

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title = "Kefiran-based scaffolds for biomedical applications",
abstract = "Kefiran is an exopolysaccharide produced by microorganisms present in kefir grains, with several health promoting properties. A optimized protocol was developed for the separation of kefiran from kefir grains, allowing to reach a yield 4÷5 {\%} without using toxic or expensive chemicals. The capability of kefiran to produce scaffold via Thermally Induced Phase Separation (TIPS) technique was investigated and porous scaffolds structure was obtained. Separated kefiran and scaffolds were analyzed via DSC and different thermal properties between purified kefiran and scaffold were revealed. XRD analysis revealed different structure between kefiran and scaffolds. The porous scaffold structure can be modulated by modifiying the thermal path of the solution during the phase separation. The citotoxicity of kefiran was evaluated with cell viability test and a significant faster cell proliferation in the treated colture medium with kefiran was evidenced.",
keywords = "Chemical Engineering (all)",
author = "Sabatino, {Maria Antonietta} and Brucato, {Valerio Maria Bartolo} and {La Carrubba}, Vincenzo and Clelia Dispenza and {Carfi' Pavia}, Francesco and Gioacchino Conoscenti and Mauro Toscano",
year = "2018",
language = "English",
volume = "64",
pages = "181--186",
journal = "CHEMICAL ENGINEERING TRANSACTIONS",
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T1 - Kefiran-based scaffolds for biomedical applications

AU - Sabatino, Maria Antonietta

AU - Brucato, Valerio Maria Bartolo

AU - La Carrubba, Vincenzo

AU - Dispenza, Clelia

AU - Carfi' Pavia, Francesco

AU - Conoscenti, Gioacchino

AU - Toscano, Mauro

PY - 2018

Y1 - 2018

N2 - Kefiran is an exopolysaccharide produced by microorganisms present in kefir grains, with several health promoting properties. A optimized protocol was developed for the separation of kefiran from kefir grains, allowing to reach a yield 4÷5 % without using toxic or expensive chemicals. The capability of kefiran to produce scaffold via Thermally Induced Phase Separation (TIPS) technique was investigated and porous scaffolds structure was obtained. Separated kefiran and scaffolds were analyzed via DSC and different thermal properties between purified kefiran and scaffold were revealed. XRD analysis revealed different structure between kefiran and scaffolds. The porous scaffold structure can be modulated by modifiying the thermal path of the solution during the phase separation. The citotoxicity of kefiran was evaluated with cell viability test and a significant faster cell proliferation in the treated colture medium with kefiran was evidenced.

AB - Kefiran is an exopolysaccharide produced by microorganisms present in kefir grains, with several health promoting properties. A optimized protocol was developed for the separation of kefiran from kefir grains, allowing to reach a yield 4÷5 % without using toxic or expensive chemicals. The capability of kefiran to produce scaffold via Thermally Induced Phase Separation (TIPS) technique was investigated and porous scaffolds structure was obtained. Separated kefiran and scaffolds were analyzed via DSC and different thermal properties between purified kefiran and scaffold were revealed. XRD analysis revealed different structure between kefiran and scaffolds. The porous scaffold structure can be modulated by modifiying the thermal path of the solution during the phase separation. The citotoxicity of kefiran was evaluated with cell viability test and a significant faster cell proliferation in the treated colture medium with kefiran was evidenced.

KW - Chemical Engineering (all)

UR - http://hdl.handle.net/10447/329945

UR - http://www.aidic.it/cet/18/64/031.pdf

M3 - Article

VL - 64

SP - 181

EP - 186

JO - CHEMICAL ENGINEERING TRANSACTIONS

JF - CHEMICAL ENGINEERING TRANSACTIONS

SN - 2283-9216

ER -