Abstract

Pesticides are generally categorized according to their great or low persistence in the environment. Although new biological, chemical, and management technologies are continually being developed to provide more sustainable production alternatives, it is expected that the use of pesticides will continue to be an essential tool in the integrated pest management. Pesticides are biologically active compounds designed to interfere with metabolic processes. Organophosphorus pesticides (OP) pesticides are less persistent than Organochlorine pesticides (OC), they are not without environmental risks, so this justifies the social concern about their level in different kind of matrices like soil, water and ground water. The aim of this study was to evaluate the biodiversity of OP degrader bacteria in Mediterranean agricultural soils. 47 isolates were obtained from enrichment cultures containing parathion or dimethoate as an exclusive carbon source. The isolates were grouped into 17 Operational Taxonomic Units (OTU) on the base of the ribosomal internal transcribed spacer (ITS) polymorphism. A positive and significative correlation was found between bacterial biodiversity and soil physical-chemistry characteristics. The strains, identified by partial sequencing of 16S rRNA gene, were tested in order to evaluate their pesticide degrading ability in mineral salt medium (MSM) by using solid-phase-micro-extraction (SPME) coupled with gas-chromatography mass-spectrometry (GC-MS). Some of the degrader isolates are not mentioned in organophosphorus degradation. Degenerated primers were used to identify the genes encoding organophosphorus hydrolase. The degradation capability of the selected strains were elucidated as possible biodegraders in contaminated standard soil microcosms, characterized by different physical chemicals characteristics.
Lingua originaleEnglish
Stato di pubblicazionePublished - 2009

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@conference{568f3a1384724694bdbbe9ff63f64b86,
title = "Isolation Of Organophosphorus Pesticides Bacteria Degraders From Agricultural Sicilian Soils.",
abstract = "Pesticides are generally categorized according to their great or low persistence in the environment. Although new biological, chemical, and management technologies are continually being developed to provide more sustainable production alternatives, it is expected that the use of pesticides will continue to be an essential tool in the integrated pest management. Pesticides are biologically active compounds designed to interfere with metabolic processes. Organophosphorus pesticides (OP) pesticides are less persistent than Organochlorine pesticides (OC), they are not without environmental risks, so this justifies the social concern about their level in different kind of matrices like soil, water and ground water. The aim of this study was to evaluate the biodiversity of OP degrader bacteria in Mediterranean agricultural soils. 47 isolates were obtained from enrichment cultures containing parathion or dimethoate as an exclusive carbon source. The isolates were grouped into 17 Operational Taxonomic Units (OTU) on the base of the ribosomal internal transcribed spacer (ITS) polymorphism. A positive and significative correlation was found between bacterial biodiversity and soil physical-chemistry characteristics. The strains, identified by partial sequencing of 16S rRNA gene, were tested in order to evaluate their pesticide degrading ability in mineral salt medium (MSM) by using solid-phase-micro-extraction (SPME) coupled with gas-chromatography mass-spectrometry (GC-MS). Some of the degrader isolates are not mentioned in organophosphorus degradation. Degenerated primers were used to identify the genes encoding organophosphorus hydrolase. The degradation capability of the selected strains were elucidated as possible biodegraders in contaminated standard soil microcosms, characterized by different physical chemicals characteristics.",
keywords = "Organophosphorus Pesticides; biodegradation; Parathion, Soil Bacteria",
author = "Giuseppe Alonzo and Eristanna Palazzolo and Paola Quatrini and {De Pasquale}, Claudio and Roberta Fodale and {Lo Piccolo}, Luca",
year = "2009",
language = "English",

}

TY - CONF

T1 - Isolation Of Organophosphorus Pesticides Bacteria Degraders From Agricultural Sicilian Soils.

AU - Alonzo, Giuseppe

AU - Palazzolo, Eristanna

AU - Quatrini, Paola

AU - De Pasquale, Claudio

AU - Fodale, Roberta

AU - Lo Piccolo, Luca

PY - 2009

Y1 - 2009

N2 - Pesticides are generally categorized according to their great or low persistence in the environment. Although new biological, chemical, and management technologies are continually being developed to provide more sustainable production alternatives, it is expected that the use of pesticides will continue to be an essential tool in the integrated pest management. Pesticides are biologically active compounds designed to interfere with metabolic processes. Organophosphorus pesticides (OP) pesticides are less persistent than Organochlorine pesticides (OC), they are not without environmental risks, so this justifies the social concern about their level in different kind of matrices like soil, water and ground water. The aim of this study was to evaluate the biodiversity of OP degrader bacteria in Mediterranean agricultural soils. 47 isolates were obtained from enrichment cultures containing parathion or dimethoate as an exclusive carbon source. The isolates were grouped into 17 Operational Taxonomic Units (OTU) on the base of the ribosomal internal transcribed spacer (ITS) polymorphism. A positive and significative correlation was found between bacterial biodiversity and soil physical-chemistry characteristics. The strains, identified by partial sequencing of 16S rRNA gene, were tested in order to evaluate their pesticide degrading ability in mineral salt medium (MSM) by using solid-phase-micro-extraction (SPME) coupled with gas-chromatography mass-spectrometry (GC-MS). Some of the degrader isolates are not mentioned in organophosphorus degradation. Degenerated primers were used to identify the genes encoding organophosphorus hydrolase. The degradation capability of the selected strains were elucidated as possible biodegraders in contaminated standard soil microcosms, characterized by different physical chemicals characteristics.

AB - Pesticides are generally categorized according to their great or low persistence in the environment. Although new biological, chemical, and management technologies are continually being developed to provide more sustainable production alternatives, it is expected that the use of pesticides will continue to be an essential tool in the integrated pest management. Pesticides are biologically active compounds designed to interfere with metabolic processes. Organophosphorus pesticides (OP) pesticides are less persistent than Organochlorine pesticides (OC), they are not without environmental risks, so this justifies the social concern about their level in different kind of matrices like soil, water and ground water. The aim of this study was to evaluate the biodiversity of OP degrader bacteria in Mediterranean agricultural soils. 47 isolates were obtained from enrichment cultures containing parathion or dimethoate as an exclusive carbon source. The isolates were grouped into 17 Operational Taxonomic Units (OTU) on the base of the ribosomal internal transcribed spacer (ITS) polymorphism. A positive and significative correlation was found between bacterial biodiversity and soil physical-chemistry characteristics. The strains, identified by partial sequencing of 16S rRNA gene, were tested in order to evaluate their pesticide degrading ability in mineral salt medium (MSM) by using solid-phase-micro-extraction (SPME) coupled with gas-chromatography mass-spectrometry (GC-MS). Some of the degrader isolates are not mentioned in organophosphorus degradation. Degenerated primers were used to identify the genes encoding organophosphorus hydrolase. The degradation capability of the selected strains were elucidated as possible biodegraders in contaminated standard soil microcosms, characterized by different physical chemicals characteristics.

KW - Organophosphorus Pesticides; biodegradation; Parathion, Soil Bacteria

UR - http://hdl.handle.net/10447/46827

M3 - Paper

ER -