Isolation and Characterization of Oil-Degrading Bacteria from Bilge Water

Santina Santisi; Gabriella Gentile; Anna Volta; Martina Bonsignore; Giuseppe Mancini; Paola Quatrini; Simone Cappello

Risultato della ricerca: Article

Abstract

Twenty-one oil-degrading bacteria were isolated from bilge water. Based on a high growth rate on crude oil and on hydrocarbon degradation ability, 7 strains were selected (from 21 isolated) for further studies. 16S rRNA gene sequencing showed that isolated strains were affiliated to Bacillus, Pseudomonas and Halomonas genera; in particular, isolate BW-B12 (Bacillus sp., 99%), BW-C12 (Halomonas boliviensis, 99%) and BW-E12 (Halomonas boliviensis, 98%) were the best crude-oil degraders; after 10 days of cultivation in ONR 7a mineral medium supplemented with crude oil as single carbon source BW-B12, BW-C12 and BW-E12 showed a degradation rate of 80, 60 and 59%, respectively. The strains showed also a high emulsification activity and biosurfactants production. Obtained results give an important contribution in order to utilize these bilge water autochthonous microorganisms in processes of bioremediation of marine environment chronically polluted from saline oily waste
Lingua originaleEnglish
pagine (da-a)45-49
Numero di pagine5
RivistaINTERNATIONAL JOURNAL OF MICROBIOLOGY AND APPLICATION
Volume2
Stato di pubblicazionePublished - 2015

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oils
bacteria
Bacillus (bacteria)
water
Halomonas
biosurfactants
degradation
bioremediation
emulsifying
marine environment
Pseudomonas
hydrocarbons
ribosomal RNA
minerals
microorganisms
carbon
genes
Halomonas boliviensis

Cita questo

Santina Santisi; Gabriella Gentile; Anna Volta; Martina Bonsignore; Giuseppe Mancini; Paola Quatrini; Simone Cappello (2015). Isolation and Characterization of Oil-Degrading Bacteria from Bilge Water. INTERNATIONAL JOURNAL OF MICROBIOLOGY AND APPLICATION, 2, 45-49.

Isolation and Characterization of Oil-Degrading Bacteria from Bilge Water. / Santina Santisi; Gabriella Gentile; Anna Volta; Martina Bonsignore; Giuseppe Mancini; Paola Quatrini; Simone Cappello.

In: INTERNATIONAL JOURNAL OF MICROBIOLOGY AND APPLICATION, Vol. 2, 2015, pag. 45-49.

Risultato della ricerca: Article

Santina Santisi; Gabriella Gentile; Anna Volta; Martina Bonsignore; Giuseppe Mancini; Paola Quatrini; Simone Cappello 2015, 'Isolation and Characterization of Oil-Degrading Bacteria from Bilge Water', INTERNATIONAL JOURNAL OF MICROBIOLOGY AND APPLICATION, vol. 2, pagg. 45-49.
Santina Santisi; Gabriella Gentile; Anna Volta; Martina Bonsignore; Giuseppe Mancini; Paola Quatrini; Simone Cappello. Isolation and Characterization of Oil-Degrading Bacteria from Bilge Water. INTERNATIONAL JOURNAL OF MICROBIOLOGY AND APPLICATION. 2015;2:45-49.
Santina Santisi; Gabriella Gentile; Anna Volta; Martina Bonsignore; Giuseppe Mancini; Paola Quatrini; Simone Cappello. / Isolation and Characterization of Oil-Degrading Bacteria from Bilge Water. In: INTERNATIONAL JOURNAL OF MICROBIOLOGY AND APPLICATION. 2015 ; Vol. 2. pagg. 45-49.
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abstract = "Twenty-one oil-degrading bacteria were isolated from bilge water. Based on a high growth rate on crude oil and on hydrocarbon degradation ability, 7 strains were selected (from 21 isolated) for further studies. 16S rRNA gene sequencing showed that isolated strains were affiliated to Bacillus, Pseudomonas and Halomonas genera; in particular, isolate BW-B12 (Bacillus sp., 99{\%}), BW-C12 (Halomonas boliviensis, 99{\%}) and BW-E12 (Halomonas boliviensis, 98{\%}) were the best crude-oil degraders; after 10 days of cultivation in ONR 7a mineral medium supplemented with crude oil as single carbon source BW-B12, BW-C12 and BW-E12 showed a degradation rate of 80, 60 and 59{\%}, respectively. The strains showed also a high emulsification activity and biosurfactants production. Obtained results give an important contribution in order to utilize these bilge water autochthonous microorganisms in processes of bioremediation of marine environment chronically polluted from saline oily waste",
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AB - Twenty-one oil-degrading bacteria were isolated from bilge water. Based on a high growth rate on crude oil and on hydrocarbon degradation ability, 7 strains were selected (from 21 isolated) for further studies. 16S rRNA gene sequencing showed that isolated strains were affiliated to Bacillus, Pseudomonas and Halomonas genera; in particular, isolate BW-B12 (Bacillus sp., 99%), BW-C12 (Halomonas boliviensis, 99%) and BW-E12 (Halomonas boliviensis, 98%) were the best crude-oil degraders; after 10 days of cultivation in ONR 7a mineral medium supplemented with crude oil as single carbon source BW-B12, BW-C12 and BW-E12 showed a degradation rate of 80, 60 and 59%, respectively. The strains showed also a high emulsification activity and biosurfactants production. Obtained results give an important contribution in order to utilize these bilge water autochthonous microorganisms in processes of bioremediation of marine environment chronically polluted from saline oily waste

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