Isolation and characterization of hydrocarbonoclastic bacteria from Algerian coast

Risultato della ricerca: Paper

Abstract

Backgrounds The contamination of marine environments by hydrocarbons represents a global concern with consequences on ecosystems and human health. The removal of HC by physical and/or chemical methods is expensive and disrespectful of the environment. The use of HC degrading bacteria is a good alternative for environmental remediation (bioremediation). Objectives The Algerian coastline is exposed to several types of pollutions, including hydrocarbons. The aim was to explore the bioremediation potential of its contaminated harbors for the first time. Methods To identify the hydrocarbon degrading bacteria seawater samples were collected in Sidi fredj harbor (Algeria) and used to enrichment cultures on mineral medium supplemented with Algerian light crude oil. The isolates were characterized by biochemical tests and Gram straining. The taxonomic identification of the isolates was performed by ARDRA and sequencing of the 16S rRNA gene. The ability to grow on hydrocarbons and degradation abilities were analysed by GC-FID together with their emulsification capacity. The key catabolic genes involved in HC degradation were detected by PCR. Conclusions About twenty isolates were assigned to known hydrocarbonoclastic genera (Alcanivorax, Halomonas, Marinobacter) and generalist HC degraders (Pseudomonas), but also to Bizionia (class Flavobacteriia). The isolates grow on n-alkanes (C16 to C24, Alcanivorax, Halomonas ) toluene and benzene (Marinobacter) and a show average oil emulsification capacity. The PCR and sequencing of catabolic genes confirmed the presence of alkane hydroxylase genes and degradation abilities analysis is in progress. Further characterization will allow us to identify the most interesting strains to perform bioremediation.
Lingua originaleEnglish
Stato di pubblicazionePublished - 2017

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bioremediation
hydrocarbon
bacterium
gene
coast
alkane
degradation
harbor
ecosystem health
chemical method
generalist
toluene
benzene
crude oil
marine environment
remediation
seawater
pollution
oil
mineral

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title = "Isolation and characterization of hydrocarbonoclastic bacteria from Algerian coast",
abstract = "Backgrounds The contamination of marine environments by hydrocarbons represents a global concern with consequences on ecosystems and human health. The removal of HC by physical and/or chemical methods is expensive and disrespectful of the environment. The use of HC degrading bacteria is a good alternative for environmental remediation (bioremediation). Objectives The Algerian coastline is exposed to several types of pollutions, including hydrocarbons. The aim was to explore the bioremediation potential of its contaminated harbors for the first time. Methods To identify the hydrocarbon degrading bacteria seawater samples were collected in Sidi fredj harbor (Algeria) and used to enrichment cultures on mineral medium supplemented with Algerian light crude oil. The isolates were characterized by biochemical tests and Gram straining. The taxonomic identification of the isolates was performed by ARDRA and sequencing of the 16S rRNA gene. The ability to grow on hydrocarbons and degradation abilities were analysed by GC-FID together with their emulsification capacity. The key catabolic genes involved in HC degradation were detected by PCR. Conclusions About twenty isolates were assigned to known hydrocarbonoclastic genera (Alcanivorax, Halomonas, Marinobacter) and generalist HC degraders (Pseudomonas), but also to Bizionia (class Flavobacteriia). The isolates grow on n-alkanes (C16 to C24, Alcanivorax, Halomonas ) toluene and benzene (Marinobacter) and a show average oil emulsification capacity. The PCR and sequencing of catabolic genes confirmed the presence of alkane hydroxylase genes and degradation abilities analysis is in progress. Further characterization will allow us to identify the most interesting strains to perform bioremediation.",
keywords = "Algerian coastline, hydrocarbon degrading bacteria, emulsification capacity, degradation ability",
author = "Paola Quatrini and Valentina Catania",
year = "2017",
language = "English",

}

TY - CONF

T1 - Isolation and characterization of hydrocarbonoclastic bacteria from Algerian coast

AU - Quatrini, Paola

AU - Catania, Valentina

PY - 2017

Y1 - 2017

N2 - Backgrounds The contamination of marine environments by hydrocarbons represents a global concern with consequences on ecosystems and human health. The removal of HC by physical and/or chemical methods is expensive and disrespectful of the environment. The use of HC degrading bacteria is a good alternative for environmental remediation (bioremediation). Objectives The Algerian coastline is exposed to several types of pollutions, including hydrocarbons. The aim was to explore the bioremediation potential of its contaminated harbors for the first time. Methods To identify the hydrocarbon degrading bacteria seawater samples were collected in Sidi fredj harbor (Algeria) and used to enrichment cultures on mineral medium supplemented with Algerian light crude oil. The isolates were characterized by biochemical tests and Gram straining. The taxonomic identification of the isolates was performed by ARDRA and sequencing of the 16S rRNA gene. The ability to grow on hydrocarbons and degradation abilities were analysed by GC-FID together with their emulsification capacity. The key catabolic genes involved in HC degradation were detected by PCR. Conclusions About twenty isolates were assigned to known hydrocarbonoclastic genera (Alcanivorax, Halomonas, Marinobacter) and generalist HC degraders (Pseudomonas), but also to Bizionia (class Flavobacteriia). The isolates grow on n-alkanes (C16 to C24, Alcanivorax, Halomonas ) toluene and benzene (Marinobacter) and a show average oil emulsification capacity. The PCR and sequencing of catabolic genes confirmed the presence of alkane hydroxylase genes and degradation abilities analysis is in progress. Further characterization will allow us to identify the most interesting strains to perform bioremediation.

AB - Backgrounds The contamination of marine environments by hydrocarbons represents a global concern with consequences on ecosystems and human health. The removal of HC by physical and/or chemical methods is expensive and disrespectful of the environment. The use of HC degrading bacteria is a good alternative for environmental remediation (bioremediation). Objectives The Algerian coastline is exposed to several types of pollutions, including hydrocarbons. The aim was to explore the bioremediation potential of its contaminated harbors for the first time. Methods To identify the hydrocarbon degrading bacteria seawater samples were collected in Sidi fredj harbor (Algeria) and used to enrichment cultures on mineral medium supplemented with Algerian light crude oil. The isolates were characterized by biochemical tests and Gram straining. The taxonomic identification of the isolates was performed by ARDRA and sequencing of the 16S rRNA gene. The ability to grow on hydrocarbons and degradation abilities were analysed by GC-FID together with their emulsification capacity. The key catabolic genes involved in HC degradation were detected by PCR. Conclusions About twenty isolates were assigned to known hydrocarbonoclastic genera (Alcanivorax, Halomonas, Marinobacter) and generalist HC degraders (Pseudomonas), but also to Bizionia (class Flavobacteriia). The isolates grow on n-alkanes (C16 to C24, Alcanivorax, Halomonas ) toluene and benzene (Marinobacter) and a show average oil emulsification capacity. The PCR and sequencing of catabolic genes confirmed the presence of alkane hydroxylase genes and degradation abilities analysis is in progress. Further characterization will allow us to identify the most interesting strains to perform bioremediation.

KW - Algerian coastline, hydrocarbon degrading bacteria, emulsification capacity, degradation ability

UR - http://hdl.handle.net/10447/242310

M3 - Paper

ER -