Isolation and characterization of a murine resident liver stem cell

Alice Conigliaro, Guerra, Leopizzi, Alice Conigliaro, Veronica Bordoni, Marta Colletti, Laura Amicone, Carla Cicchini, Marco Tripodi, Manfredini, Siepi, Zini

Risultato della ricerca: Articlepeer review

28 Citazioni (Scopus)

Abstract

Increasing evidence provides support that mammalian liver contains stem/progenitor cells, but their molecular phenotype, embryological derivation, biology and their role in liver cell turnover and regeneration remain to be further clarified. In this study, we report the isolation, characterization and reproducible establishment in line of a resident liver stem cell (RLSC) with immunophenotype and differentiative potentiality distinct from other previously described liver precursor/stem cells. RLSCs, derived from fetal and neonatal murine livers as well as from immortalized hepatocytic MMH lines and established in lines, are Sca+, CD34-, CD45-, α-fetoprotein+ and albumin-. This molecular phenotype suggests a non-hematopoietic origin. RLSC transcriptional profile, defined by microArray technology, highlighted the expression of a broad spectrum of 'plasticity-related genes' and 'developmental genes' suggesting a multi-differentiative potentiality. Indeed, RLSCs spontaneously differentiate into hepatocytes and cholangiocytes and, when cultured in appropriate conditions, into mesenchymal and neuro-ectodermal cell lineages such as osteoblasts/ osteocytes, chondrocytes, astrocytes and neural cells. RLSC capability to spontaneously differentiate into hepatocytes, the lack of albumin expression and the broad differentiative potentiality locate them in a pre-hepatoblast/liver precursor cells hierarchical position. In conclusion, RLSCs may provide a useful tool to improve liver stem cell knowledge and to assess new therapeutic approaches for liver diseases.
Lingua originaleEnglish
pagine (da-a)123-133
Numero di pagine11
RivistaCell Death and Differentiation
Volume15
Stato di pubblicazionePublished - 2008

All Science Journal Classification (ASJC) codes

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