Introduction: Mesenchymal Stromal Cells (MSCs) can beisolated from adipose tissue and are currently used in vitrofor experimental studies. MSCs are often mislabeled asAdipose-derived Stem Cells (ADSCs) despite presenting amore differentiated phenotype. The isolation and expansionof non-adherent progenitors from adipose-derived stem cells(napADSCs), has been achieved in our laboratory and iscurrently under investigation. In this study we investigate:a) the proof of stemness of such progenitors, and b) thefeasibility of napADSCs adhesion over Integra® for cellcolonization and for future differentiation and engineering ofsemi-synthetic tissues.Material and Methods: Adipose tissue (20 cc) was extractedfrom lipoaspirate samples of 15 healthy donors followingpatients written consent. Following mechanical and enzymaticdigestion, samples were plated in stem cell-specific enrichedmedia and in no adherence conditions. Clonal expansion of asingle cell was assessed by limiting dilution and asymmetricdivision was detected by PKH26 staining. Expanded cellswere seeded within Integra® in 24-well plates.Results: NapADSCs represent an upstream line ofmesenchymal progenitors compared to more differentiated,adherent, fibroblast-like MSCs. NapADSCs colonies definedas spheroids (polyclonal) and sphere-derived clonogenic cells(monoclonal) were visible in 1-3 weeks and their stemnessconfirmed in vitro by clonal expansion. NapADSCs adhesionto Integra® was achieved modifying culturing conditions,and was visible in 3-7 days with phenotype change fromnapADSCs to fibroblast-like MSCs.Conclusions: Our data suggests that the identification ofnapADSCs may dissipate the doubts on the stem-cell originof the more differentiated and commonly used adherentMSCs. The ability of napADSCs to adhere to a clinicallyavailable dermal regenerative template, and grow within itsthree-dimensional structure, may prove useful in regenerativesurgery. Further studies are warranted to assess whethersuch procedure may represent a first preliminary step towardclinical application of semi-synthetic tissues.
|Numero di pagine||1|
|Rivista||Plastic and Reconstructive Surgery|
|Stato di pubblicazione||Published - 2012|