Abstract

Background: Long Non-coding RNAs (lncRNA) are emerging as essential regulators of genetic and epigenetic networks, and their deregulation may underlie complex diseases, such as carcinogenesis. Several studies have described lncRNAs alterations in patients with solid tumors. In particular, in Gastrointestinal Stromal Tumors (GIST), upregulation of HOTAIR has been associated with aggressiveness, metastasis, and poor patients’ survival. In order to gain more detailed insight on the molecular role of lncRNAs in GIST, we analyzed in vivo the expression levels of lncRNAs H19 and MALAT1 in surgically resected patients. Material and Methods: The expression of the lnc-RNAs H19 and MALAT1 was evaluated in primary tumor tissue from 20 GIST patients undergoing surgical resection, and paired normal mucosa samples, using quantitative real-time reverse transcriptase qRT-PCR. The result was considered reliable if the tumor tissue harboured at least 70% of cancer cells. Results: H19 was evaluable in 20 patients, MALAT 1 in 8 patients. H19 was overexpressed in 66% (12/20) cancer tissue from GIST patients, and the difference of expression between the two groups (tumor tissue vs normal tissue) was found to be statistically significant (P= 0.0496). MALAT1 was overexpressed also in 100% (8/8) cancer tissue from GIST patients. Conclusions: H19 and MALAT1 appear frequently upregulated in GIST patients. Further analyses are needed to confirm these data, and evaluate the potential role of such lncRNAs, as prognostic/predictive biomarkers.
Lingua originaleEnglish
Stato di pubblicazionePublished - 2015

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Long Noncoding RNA
Gastrointestinal Stromal Tumors
Biomarkers
Neoplasms
Epigenomics
Inpatients
Carcinogenesis
Mucous Membrane
Up-Regulation
Neoplasm Metastasis
Polymerase Chain Reaction
Survival

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@conference{cc521563f1be435ba2d3c2574c6f51f2,
title = "Into the wild of long non-coding RNA in gastrointestinal stromal tumors (GISTs) to explore new prognostic/predictive biomarkers",
abstract = "Background: Long Non-coding RNAs (lncRNA) are emerging as essential regulators of genetic and epigenetic networks, and their deregulation may underlie complex diseases, such as carcinogenesis. Several studies have described lncRNAs alterations in patients with solid tumors. In particular, in Gastrointestinal Stromal Tumors (GIST), upregulation of HOTAIR has been associated with aggressiveness, metastasis, and poor patients’ survival. In order to gain more detailed insight on the molecular role of lncRNAs in GIST, we analyzed in vivo the expression levels of lncRNAs H19 and MALAT1 in surgically resected patients. Material and Methods: The expression of the lnc-RNAs H19 and MALAT1 was evaluated in primary tumor tissue from 20 GIST patients undergoing surgical resection, and paired normal mucosa samples, using quantitative real-time reverse transcriptase qRT-PCR. The result was considered reliable if the tumor tissue harboured at least 70{\%} of cancer cells. Results: H19 was evaluable in 20 patients, MALAT 1 in 8 patients. H19 was overexpressed in 66{\%} (12/20) cancer tissue from GIST patients, and the difference of expression between the two groups (tumor tissue vs normal tissue) was found to be statistically significant (P= 0.0496). MALAT1 was overexpressed also in 100{\%} (8/8) cancer tissue from GIST patients. Conclusions: H19 and MALAT1 appear frequently upregulated in GIST patients. Further analyses are needed to confirm these data, and evaluate the potential role of such lncRNAs, as prognostic/predictive biomarkers.",
author = "Antonio Russo and Viviana Bazan and Giuseppe Badalamenti and Fabio Fulfaro and Giuseppe Bronte and Lorena Incorvaia and Lavinia Insalaco and Marta Castiglia and Enrico Bronte and Antonina Cangemi and Nadia Barraco and Alessandro Perez",
year = "2015",
language = "English",

}

TY - CONF

T1 - Into the wild of long non-coding RNA in gastrointestinal stromal tumors (GISTs) to explore new prognostic/predictive biomarkers

AU - Russo, Antonio

AU - Bazan, Viviana

AU - Badalamenti, Giuseppe

AU - Fulfaro, Fabio

AU - Bronte, Giuseppe

AU - Incorvaia, Lorena

AU - Insalaco, Lavinia

AU - Castiglia, Marta

AU - Bronte, Enrico

AU - Cangemi, Antonina

AU - Barraco, Nadia

AU - Perez, Alessandro

PY - 2015

Y1 - 2015

N2 - Background: Long Non-coding RNAs (lncRNA) are emerging as essential regulators of genetic and epigenetic networks, and their deregulation may underlie complex diseases, such as carcinogenesis. Several studies have described lncRNAs alterations in patients with solid tumors. In particular, in Gastrointestinal Stromal Tumors (GIST), upregulation of HOTAIR has been associated with aggressiveness, metastasis, and poor patients’ survival. In order to gain more detailed insight on the molecular role of lncRNAs in GIST, we analyzed in vivo the expression levels of lncRNAs H19 and MALAT1 in surgically resected patients. Material and Methods: The expression of the lnc-RNAs H19 and MALAT1 was evaluated in primary tumor tissue from 20 GIST patients undergoing surgical resection, and paired normal mucosa samples, using quantitative real-time reverse transcriptase qRT-PCR. The result was considered reliable if the tumor tissue harboured at least 70% of cancer cells. Results: H19 was evaluable in 20 patients, MALAT 1 in 8 patients. H19 was overexpressed in 66% (12/20) cancer tissue from GIST patients, and the difference of expression between the two groups (tumor tissue vs normal tissue) was found to be statistically significant (P= 0.0496). MALAT1 was overexpressed also in 100% (8/8) cancer tissue from GIST patients. Conclusions: H19 and MALAT1 appear frequently upregulated in GIST patients. Further analyses are needed to confirm these data, and evaluate the potential role of such lncRNAs, as prognostic/predictive biomarkers.

AB - Background: Long Non-coding RNAs (lncRNA) are emerging as essential regulators of genetic and epigenetic networks, and their deregulation may underlie complex diseases, such as carcinogenesis. Several studies have described lncRNAs alterations in patients with solid tumors. In particular, in Gastrointestinal Stromal Tumors (GIST), upregulation of HOTAIR has been associated with aggressiveness, metastasis, and poor patients’ survival. In order to gain more detailed insight on the molecular role of lncRNAs in GIST, we analyzed in vivo the expression levels of lncRNAs H19 and MALAT1 in surgically resected patients. Material and Methods: The expression of the lnc-RNAs H19 and MALAT1 was evaluated in primary tumor tissue from 20 GIST patients undergoing surgical resection, and paired normal mucosa samples, using quantitative real-time reverse transcriptase qRT-PCR. The result was considered reliable if the tumor tissue harboured at least 70% of cancer cells. Results: H19 was evaluable in 20 patients, MALAT 1 in 8 patients. H19 was overexpressed in 66% (12/20) cancer tissue from GIST patients, and the difference of expression between the two groups (tumor tissue vs normal tissue) was found to be statistically significant (P= 0.0496). MALAT1 was overexpressed also in 100% (8/8) cancer tissue from GIST patients. Conclusions: H19 and MALAT1 appear frequently upregulated in GIST patients. Further analyses are needed to confirm these data, and evaluate the potential role of such lncRNAs, as prognostic/predictive biomarkers.

UR - http://hdl.handle.net/10447/176224

M3 - Paper

ER -