Interleukin 3- receptor targeted exosomes inhibit in vitro and in vivo chronic myelogenous Leukemia cell growth

Riccardo Alessandro, Marta Cristaldi, Stefania Raimondo, Simona Fontana, Girolamo Cirrincione, Patrizia Diana, Francesca Monteleone, Daniele Bellavia, Mauro Manno, Stefano Forte, Samuele Raccosta, Giovanna Calabrese, Lorenzo Memeo, Gianluca Giavaresi, Daniele Bellavia, Mauro Manno, Samuele Raccosta

Risultato della ricerca: Articlepeer review

79 Citazioni (Scopus)

Abstract

Despite Imatinib (IM), a selective inhibitor of Bcr-Abl, having led to improved prognosis in Chronic Myeloid Leukemia (CML) patients, acquired resistance and long-term adverse effects is still being encountered. There is, therefore, urgent need to develop alternative strategies to overcome drug resistance. According to the molecules expressed on their surface, exosomes can target specific cells. Exosomes can also be loaded with a variety of molecules, thereby acting as a vehicle for the delivery of therapeutic agents. In this study, we engineered HEK293T cells to express the exosomal protein Lamp2b, fused to a fragment of Interleukin 3 (IL3). The IL3 receptor (IL3-R) is overexpressed in CML blasts compared to normal hematopoietic cells and thus is able to act as a receptor target in a cancer drug delivery system. Here we show that IL3L exosomes, loaded with Imatinib or with BCR-ABL siRNA, are able to target CML cells and inhibit in vitro and in vivo cancer cell growth.
Lingua originaleEnglish
pagine (da-a)1333-1345
Numero di pagine13
RivistaTheranostics
Volume7
Stato di pubblicazionePublished - 2017

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

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