Inflammation is a complex reaction of hostdefence mechanisms aiming at neutralization of aninsult and restoring normal tissue structure andfunction. In human IL-17 is T-cell derived cytokineplays a key role in the clearance of extracellularbacteria promoting cell infiltration and production ofseveral cytokines and chemokines. Here, we reporton three Ciona intestinalis IL-17 homologues(CiIL17-1, CiIL17-2, CiIL17-3). The geneorganization, phylogenetic tree and modelingsupported the close relationship with themammalian IL-17A and IL-17F suggesting that theC. intestinalis IL-17 genes share a commonancestor in the chordate lineages. Real time PCRanalysis showed a prompt expression induced byLPS inoculation showing that they are involved inthe first steps of inflammatory response. In situhybridization assays disclosed that the genes transcription was upregulated in the pharynx, themain organ of the ascidian immune system, andexpressed by hemocytes (granulocytes andunivacuolar refractile granulocyte) inside thepharynx vessels. As in human, we can assume thatCiIL-17-like stimulates the release of CiTNFalpha,which synergizes with CiIL-17 in its effects on cellsand molecules of Ciona intestinalis immunitysystem. In addition, a comparative evaluation withothers molecules upregulated by LPS challenge asCiTNF-alpha, phenoloxisases, peroxinectin,galectins and mannan binding lectin, have beenevaluated in terms of temporal and quantitativegene expression.
|Numero di pagine||1|
|Stato di pubblicazione||Published - 2015|